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Prevention of Aflatoxin B(1)-Induced DNA Breaks by β-D-Glucan

Aflatoxins are a group of naturally-occurring carcinogens that are known to contaminate different human and animal foodstuffs. Aflatoxin B(1) (AFB(1)) is the most genotoxic hepatocarcinogenic compound of all of the aflatoxins. In this report, we explore the capacity of β-d-glucan (Glu) to reduce the...

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Autores principales: Madrigal-Bujaidar, Eduardo, Morales-González, José Antonio, Sánchez-Gutiérrez, Manuel, Izquierdo-Vega, Jeannett A., Reyes-Arellano, Alicia, Álvarez-González, Isela, Pérez-Pasten, Ricardo, Madrigal-Santillán, Eduardo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488695/
https://www.ncbi.nlm.nih.gov/pubmed/26110504
http://dx.doi.org/10.3390/toxins7062145
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author Madrigal-Bujaidar, Eduardo
Morales-González, José Antonio
Sánchez-Gutiérrez, Manuel
Izquierdo-Vega, Jeannett A.
Reyes-Arellano, Alicia
Álvarez-González, Isela
Pérez-Pasten, Ricardo
Madrigal-Santillán, Eduardo
author_facet Madrigal-Bujaidar, Eduardo
Morales-González, José Antonio
Sánchez-Gutiérrez, Manuel
Izquierdo-Vega, Jeannett A.
Reyes-Arellano, Alicia
Álvarez-González, Isela
Pérez-Pasten, Ricardo
Madrigal-Santillán, Eduardo
author_sort Madrigal-Bujaidar, Eduardo
collection PubMed
description Aflatoxins are a group of naturally-occurring carcinogens that are known to contaminate different human and animal foodstuffs. Aflatoxin B(1) (AFB(1)) is the most genotoxic hepatocarcinogenic compound of all of the aflatoxins. In this report, we explore the capacity of β-d-glucan (Glu) to reduce the DNA damage induced by AFB(1) in mouse hepatocytes. For this purpose, we applied the comet assay to groups of animals that were first administered Glu in three doses (100, 400 and 700 mg/kg bw, respectively) and, 20 min later, 1.0 mg/kg of AFB(1). Liver cells were obtained at 4, 10 and 16 h after the chemical administration and examined. The results showed no protection of the damage induced by AFB(1) with the low dose of the polysaccharide, but they did reveal antigenotoxic activity exerted by the two high doses. In addition, we induced a co-crystallization between both compounds, determined their fusion points and analyzed the molecules by UV spectroscopy. The data suggested the formation of a supramolecular complex between AFB(1) and β-d-glucan.
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spelling pubmed-44886952015-07-06 Prevention of Aflatoxin B(1)-Induced DNA Breaks by β-D-Glucan Madrigal-Bujaidar, Eduardo Morales-González, José Antonio Sánchez-Gutiérrez, Manuel Izquierdo-Vega, Jeannett A. Reyes-Arellano, Alicia Álvarez-González, Isela Pérez-Pasten, Ricardo Madrigal-Santillán, Eduardo Toxins (Basel) Article Aflatoxins are a group of naturally-occurring carcinogens that are known to contaminate different human and animal foodstuffs. Aflatoxin B(1) (AFB(1)) is the most genotoxic hepatocarcinogenic compound of all of the aflatoxins. In this report, we explore the capacity of β-d-glucan (Glu) to reduce the DNA damage induced by AFB(1) in mouse hepatocytes. For this purpose, we applied the comet assay to groups of animals that were first administered Glu in three doses (100, 400 and 700 mg/kg bw, respectively) and, 20 min later, 1.0 mg/kg of AFB(1). Liver cells were obtained at 4, 10 and 16 h after the chemical administration and examined. The results showed no protection of the damage induced by AFB(1) with the low dose of the polysaccharide, but they did reveal antigenotoxic activity exerted by the two high doses. In addition, we induced a co-crystallization between both compounds, determined their fusion points and analyzed the molecules by UV spectroscopy. The data suggested the formation of a supramolecular complex between AFB(1) and β-d-glucan. MDPI 2015-06-11 /pmc/articles/PMC4488695/ /pubmed/26110504 http://dx.doi.org/10.3390/toxins7062145 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Madrigal-Bujaidar, Eduardo
Morales-González, José Antonio
Sánchez-Gutiérrez, Manuel
Izquierdo-Vega, Jeannett A.
Reyes-Arellano, Alicia
Álvarez-González, Isela
Pérez-Pasten, Ricardo
Madrigal-Santillán, Eduardo
Prevention of Aflatoxin B(1)-Induced DNA Breaks by β-D-Glucan
title Prevention of Aflatoxin B(1)-Induced DNA Breaks by β-D-Glucan
title_full Prevention of Aflatoxin B(1)-Induced DNA Breaks by β-D-Glucan
title_fullStr Prevention of Aflatoxin B(1)-Induced DNA Breaks by β-D-Glucan
title_full_unstemmed Prevention of Aflatoxin B(1)-Induced DNA Breaks by β-D-Glucan
title_short Prevention of Aflatoxin B(1)-Induced DNA Breaks by β-D-Glucan
title_sort prevention of aflatoxin b(1)-induced dna breaks by β-d-glucan
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488695/
https://www.ncbi.nlm.nih.gov/pubmed/26110504
http://dx.doi.org/10.3390/toxins7062145
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