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Carbon Quantum Dots for Zebrafish Fluorescence Imaging

Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based QDs and dye probes owing to their high biocompatibility, low toxicity, ease of preparation, and unique photophysical properties. Herein, we describe fluorescence bioimaging of zebrafish using C-QDs as probe in terms of t...

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Autores principales: Kang, Yan-Fei, Li, Yu-Hao, Fang, Yang-Wu, Xu, Yang, Wei, Xiao-Mi, Yin, Xue-Bo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488761/
https://www.ncbi.nlm.nih.gov/pubmed/26135470
http://dx.doi.org/10.1038/srep11835
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author Kang, Yan-Fei
Li, Yu-Hao
Fang, Yang-Wu
Xu, Yang
Wei, Xiao-Mi
Yin, Xue-Bo
author_facet Kang, Yan-Fei
Li, Yu-Hao
Fang, Yang-Wu
Xu, Yang
Wei, Xiao-Mi
Yin, Xue-Bo
author_sort Kang, Yan-Fei
collection PubMed
description Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based QDs and dye probes owing to their high biocompatibility, low toxicity, ease of preparation, and unique photophysical properties. Herein, we describe fluorescence bioimaging of zebrafish using C-QDs as probe in terms of the preparation of C-QDs, zebrafish husbandry, embryo harvesting, and introduction of C-QDs into embryos and larvae by soaking and microinjection. The multicolor of C-QDs was validated with their imaging for zebrafish embryo. The distribution of C-QDs in zebrafish embryos and larvae were successfully observed from their fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as model and C-QDs do not interfere to the development of zebrafish embryo. All of the results confirmed the high biocompatibility and low toxicity of C-QDs as imaging probe. The absorption, distribution, metabolism and excretion route (ADME) of C-QDs in zebrafish was revealed by their distribution. Our work provides the useful information for the researchers interested in studying with zebrafish as a model and the applications of C-QDs. The operations related zebrafish are suitable for the study of the toxicity, adverse effects, transport, and biocompatibility of nanomaterials as well as for drug screening with zebrafish as model.
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spelling pubmed-44887612015-07-08 Carbon Quantum Dots for Zebrafish Fluorescence Imaging Kang, Yan-Fei Li, Yu-Hao Fang, Yang-Wu Xu, Yang Wei, Xiao-Mi Yin, Xue-Bo Sci Rep Article Carbon quantum dots (C-QDs) are becoming a desirable alternative to metal-based QDs and dye probes owing to their high biocompatibility, low toxicity, ease of preparation, and unique photophysical properties. Herein, we describe fluorescence bioimaging of zebrafish using C-QDs as probe in terms of the preparation of C-QDs, zebrafish husbandry, embryo harvesting, and introduction of C-QDs into embryos and larvae by soaking and microinjection. The multicolor of C-QDs was validated with their imaging for zebrafish embryo. The distribution of C-QDs in zebrafish embryos and larvae were successfully observed from their fluorescence emission. the bio-toxicity of C-QDs was tested with zebrafish as model and C-QDs do not interfere to the development of zebrafish embryo. All of the results confirmed the high biocompatibility and low toxicity of C-QDs as imaging probe. The absorption, distribution, metabolism and excretion route (ADME) of C-QDs in zebrafish was revealed by their distribution. Our work provides the useful information for the researchers interested in studying with zebrafish as a model and the applications of C-QDs. The operations related zebrafish are suitable for the study of the toxicity, adverse effects, transport, and biocompatibility of nanomaterials as well as for drug screening with zebrafish as model. Nature Publishing Group 2015-07-02 /pmc/articles/PMC4488761/ /pubmed/26135470 http://dx.doi.org/10.1038/srep11835 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Kang, Yan-Fei
Li, Yu-Hao
Fang, Yang-Wu
Xu, Yang
Wei, Xiao-Mi
Yin, Xue-Bo
Carbon Quantum Dots for Zebrafish Fluorescence Imaging
title Carbon Quantum Dots for Zebrafish Fluorescence Imaging
title_full Carbon Quantum Dots for Zebrafish Fluorescence Imaging
title_fullStr Carbon Quantum Dots for Zebrafish Fluorescence Imaging
title_full_unstemmed Carbon Quantum Dots for Zebrafish Fluorescence Imaging
title_short Carbon Quantum Dots for Zebrafish Fluorescence Imaging
title_sort carbon quantum dots for zebrafish fluorescence imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488761/
https://www.ncbi.nlm.nih.gov/pubmed/26135470
http://dx.doi.org/10.1038/srep11835
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