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NF-κBp65 and Expression of Its Pro-Inflammatory Target Genes Are Upregulated in the Subcutaneous Adipose Tissue of Cachectic Cancer Patients

Cancer cachexia, of which the most notable symptom is severe and rapid weight loss, is present in the majority of patients with advanced cancer. Inflammatory mediators play an important role in the development of cachexia, envisaged as a chronic inflammatory syndrome. The white adipose tissue (WAT)...

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Autores principales: Gonzalez Camargo, Rodolfo, Mendes dos Reis Riccardi, Daniela, Quintas Teixeira Ribeiro, Henrique, Carlos Carnevali, Luiz, Marques de Matos-Neto, Emidio, Enjiu, Lucas, Xavier Neves, Rodrigo, Darck Carola Correia Lima, Joanna, Galvão Figuerêdo, Raquel, Sérgio Martins de Alcântara, Paulo, Maximiano, Linda, Otoch, José, Batista, Miguel Luiz, Püschel, Gerhard, Seelaender, Marilia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488796/
https://www.ncbi.nlm.nih.gov/pubmed/26053616
http://dx.doi.org/10.3390/nu7064465
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author Gonzalez Camargo, Rodolfo
Mendes dos Reis Riccardi, Daniela
Quintas Teixeira Ribeiro, Henrique
Carlos Carnevali, Luiz
Marques de Matos-Neto, Emidio
Enjiu, Lucas
Xavier Neves, Rodrigo
Darck Carola Correia Lima, Joanna
Galvão Figuerêdo, Raquel
Sérgio Martins de Alcântara, Paulo
Maximiano, Linda
Otoch, José
Batista, Miguel Luiz
Püschel, Gerhard
Seelaender, Marilia
author_facet Gonzalez Camargo, Rodolfo
Mendes dos Reis Riccardi, Daniela
Quintas Teixeira Ribeiro, Henrique
Carlos Carnevali, Luiz
Marques de Matos-Neto, Emidio
Enjiu, Lucas
Xavier Neves, Rodrigo
Darck Carola Correia Lima, Joanna
Galvão Figuerêdo, Raquel
Sérgio Martins de Alcântara, Paulo
Maximiano, Linda
Otoch, José
Batista, Miguel Luiz
Püschel, Gerhard
Seelaender, Marilia
author_sort Gonzalez Camargo, Rodolfo
collection PubMed
description Cancer cachexia, of which the most notable symptom is severe and rapid weight loss, is present in the majority of patients with advanced cancer. Inflammatory mediators play an important role in the development of cachexia, envisaged as a chronic inflammatory syndrome. The white adipose tissue (WAT) is one of the first compartments affected in cancer cachexia and suffers a high rate of lipolysis. It secretes several cytokines capable of directly regulating intermediate metabolism. A common pathway in the regulation of the expression of pro-inflammatory cytokines in WAT is the activation of the nuclear transcription factor kappa-B (NF-κB). We have examined the gene expression of the subunits NF-κBp65 and NF-κBp50, as well as NF-κBp65 and NF-κBp50 binding, the gene expression of pro-inflammatory mediators under NF-κB control (IL-1β, IL-6, INF-γ, TNF-α, MCP-1), and its inhibitory protein, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκB-α). The observational study involved 35 patients (control group, n = 12 and cancer group, n = 23, further divided into cachectic and non-cachectic). NF-κBp65 and its target genes expression (TNF-α, IL-1β, MCP-1 and IκB-α) were significantly higher in cachectic cancer patients. Moreover, NF-κBp65 gene expression correlated positively with the expression of its target genes. The results strongly suggest that the NF-κB pathway plays a role in the promotion of WAT inflammation during cachexia.
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spelling pubmed-44887962015-07-02 NF-κBp65 and Expression of Its Pro-Inflammatory Target Genes Are Upregulated in the Subcutaneous Adipose Tissue of Cachectic Cancer Patients Gonzalez Camargo, Rodolfo Mendes dos Reis Riccardi, Daniela Quintas Teixeira Ribeiro, Henrique Carlos Carnevali, Luiz Marques de Matos-Neto, Emidio Enjiu, Lucas Xavier Neves, Rodrigo Darck Carola Correia Lima, Joanna Galvão Figuerêdo, Raquel Sérgio Martins de Alcântara, Paulo Maximiano, Linda Otoch, José Batista, Miguel Luiz Püschel, Gerhard Seelaender, Marilia Nutrients Article Cancer cachexia, of which the most notable symptom is severe and rapid weight loss, is present in the majority of patients with advanced cancer. Inflammatory mediators play an important role in the development of cachexia, envisaged as a chronic inflammatory syndrome. The white adipose tissue (WAT) is one of the first compartments affected in cancer cachexia and suffers a high rate of lipolysis. It secretes several cytokines capable of directly regulating intermediate metabolism. A common pathway in the regulation of the expression of pro-inflammatory cytokines in WAT is the activation of the nuclear transcription factor kappa-B (NF-κB). We have examined the gene expression of the subunits NF-κBp65 and NF-κBp50, as well as NF-κBp65 and NF-κBp50 binding, the gene expression of pro-inflammatory mediators under NF-κB control (IL-1β, IL-6, INF-γ, TNF-α, MCP-1), and its inhibitory protein, nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (IκB-α). The observational study involved 35 patients (control group, n = 12 and cancer group, n = 23, further divided into cachectic and non-cachectic). NF-κBp65 and its target genes expression (TNF-α, IL-1β, MCP-1 and IκB-α) were significantly higher in cachectic cancer patients. Moreover, NF-κBp65 gene expression correlated positively with the expression of its target genes. The results strongly suggest that the NF-κB pathway plays a role in the promotion of WAT inflammation during cachexia. MDPI 2015-06-04 /pmc/articles/PMC4488796/ /pubmed/26053616 http://dx.doi.org/10.3390/nu7064465 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gonzalez Camargo, Rodolfo
Mendes dos Reis Riccardi, Daniela
Quintas Teixeira Ribeiro, Henrique
Carlos Carnevali, Luiz
Marques de Matos-Neto, Emidio
Enjiu, Lucas
Xavier Neves, Rodrigo
Darck Carola Correia Lima, Joanna
Galvão Figuerêdo, Raquel
Sérgio Martins de Alcântara, Paulo
Maximiano, Linda
Otoch, José
Batista, Miguel Luiz
Püschel, Gerhard
Seelaender, Marilia
NF-κBp65 and Expression of Its Pro-Inflammatory Target Genes Are Upregulated in the Subcutaneous Adipose Tissue of Cachectic Cancer Patients
title NF-κBp65 and Expression of Its Pro-Inflammatory Target Genes Are Upregulated in the Subcutaneous Adipose Tissue of Cachectic Cancer Patients
title_full NF-κBp65 and Expression of Its Pro-Inflammatory Target Genes Are Upregulated in the Subcutaneous Adipose Tissue of Cachectic Cancer Patients
title_fullStr NF-κBp65 and Expression of Its Pro-Inflammatory Target Genes Are Upregulated in the Subcutaneous Adipose Tissue of Cachectic Cancer Patients
title_full_unstemmed NF-κBp65 and Expression of Its Pro-Inflammatory Target Genes Are Upregulated in the Subcutaneous Adipose Tissue of Cachectic Cancer Patients
title_short NF-κBp65 and Expression of Its Pro-Inflammatory Target Genes Are Upregulated in the Subcutaneous Adipose Tissue of Cachectic Cancer Patients
title_sort nf-κbp65 and expression of its pro-inflammatory target genes are upregulated in the subcutaneous adipose tissue of cachectic cancer patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488796/
https://www.ncbi.nlm.nih.gov/pubmed/26053616
http://dx.doi.org/10.3390/nu7064465
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