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Chromosomal organization of the ribosomal RNA genes in the genus Chironomus (Diptera, Chironomidae)

Abstract. Chromosomal localization of ribosomal RNA coding genes has been studied by using FISH (fluorescence in situ hybridization) in 21 species from the genus Chironomus Meigen, 1803. Analysis of the data has shown intra- and interspecific variation in number and location of 5.8S rDNA hybridizati...

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Autores principales: Gunderina, Larisa, Golygina, Veronika, Broshkov, Andrey
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Pensoft Publishers 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488967/
https://www.ncbi.nlm.nih.gov/pubmed/26140162
http://dx.doi.org/10.3897/CompCytogen.v9i2.9055
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author Gunderina, Larisa
Golygina, Veronika
Broshkov, Andrey
author_facet Gunderina, Larisa
Golygina, Veronika
Broshkov, Andrey
author_sort Gunderina, Larisa
collection PubMed
description Abstract. Chromosomal localization of ribosomal RNA coding genes has been studied by using FISH (fluorescence in situ hybridization) in 21 species from the genus Chironomus Meigen, 1803. Analysis of the data has shown intra- and interspecific variation in number and location of 5.8S rDNA hybridization sites in 17 species from the subgenus Chironomus and 4 species from the subgenus Camptochironomus Kieffer, 1914. In the majority of studied species the location of rDNA sites coincided with the sites where active NORs (nucleolus organizer regions) were found. The number of hybridization sites in karyotypes of studied chironomids varied from 1 to 6. More than half of the species possessed only one NOR (12 out of 21). Two rDNA hybridization sites were found in karyotypes of five species, three – in two species, and five and six sites – in one species each. NORs were found in all chromosomal arms of species from the subgenus Chironomus with one of them always located on arm G. On the other hand, no hybridization sites were found on arm G in four studied species from the subgenus Camptochironomus. Two species from the subgenus Chironomus – Chironomus balatonicus Devai, Wuelker & Scholl, 1983 and Chironomus “annularius” sensu Strenzke, 1959 – showed intraspecific variability in the number of hybridization signals. Possible mechanisms of origin of variability in number and location of rRNA genes in the karyotypes of species from the genus Chironomus are discussed.
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spelling pubmed-44889672015-07-02 Chromosomal organization of the ribosomal RNA genes in the genus Chironomus (Diptera, Chironomidae) Gunderina, Larisa Golygina, Veronika Broshkov, Andrey Comp Cytogenet Research Articles Abstract. Chromosomal localization of ribosomal RNA coding genes has been studied by using FISH (fluorescence in situ hybridization) in 21 species from the genus Chironomus Meigen, 1803. Analysis of the data has shown intra- and interspecific variation in number and location of 5.8S rDNA hybridization sites in 17 species from the subgenus Chironomus and 4 species from the subgenus Camptochironomus Kieffer, 1914. In the majority of studied species the location of rDNA sites coincided with the sites where active NORs (nucleolus organizer regions) were found. The number of hybridization sites in karyotypes of studied chironomids varied from 1 to 6. More than half of the species possessed only one NOR (12 out of 21). Two rDNA hybridization sites were found in karyotypes of five species, three – in two species, and five and six sites – in one species each. NORs were found in all chromosomal arms of species from the subgenus Chironomus with one of them always located on arm G. On the other hand, no hybridization sites were found on arm G in four studied species from the subgenus Camptochironomus. Two species from the subgenus Chironomus – Chironomus balatonicus Devai, Wuelker & Scholl, 1983 and Chironomus “annularius” sensu Strenzke, 1959 – showed intraspecific variability in the number of hybridization signals. Possible mechanisms of origin of variability in number and location of rRNA genes in the karyotypes of species from the genus Chironomus are discussed. Pensoft Publishers 2015-05-22 /pmc/articles/PMC4488967/ /pubmed/26140162 http://dx.doi.org/10.3897/CompCytogen.v9i2.9055 Text en Larisa Gunderina, Veronika Golygina, Andrey Broshkov http://creativecommons.org/licenses/by/4.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Articles
Gunderina, Larisa
Golygina, Veronika
Broshkov, Andrey
Chromosomal organization of the ribosomal RNA genes in the genus Chironomus (Diptera, Chironomidae)
title Chromosomal organization of the ribosomal RNA genes in the genus Chironomus (Diptera, Chironomidae)
title_full Chromosomal organization of the ribosomal RNA genes in the genus Chironomus (Diptera, Chironomidae)
title_fullStr Chromosomal organization of the ribosomal RNA genes in the genus Chironomus (Diptera, Chironomidae)
title_full_unstemmed Chromosomal organization of the ribosomal RNA genes in the genus Chironomus (Diptera, Chironomidae)
title_short Chromosomal organization of the ribosomal RNA genes in the genus Chironomus (Diptera, Chironomidae)
title_sort chromosomal organization of the ribosomal rna genes in the genus chironomus (diptera, chironomidae)
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4488967/
https://www.ncbi.nlm.nih.gov/pubmed/26140162
http://dx.doi.org/10.3897/CompCytogen.v9i2.9055
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AT broshkovandrey chromosomalorganizationoftheribosomalrnagenesinthegenuschironomusdipterachironomidae