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Mouse Hepatic Tumor Vascular Imaging by Experimental Selective Angiography

PURPOSE: Human hepatocellular carcinoma (HCC) has unique vascular features, which require selective imaging of hepatic arterial perfusion and portal venous perfusion with vascular catheterization for sufficient evaluation. Unlike in humans, vessels in mice are too small to catheterize, and the impor...

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Autores principales: Kim, Sang Kyum, Kim, Honsoul, Koh, Gou Young, Lim, Dae-Sik, Yu, Dae-Yeul, Kim, Man Deuk, Park, Mi-Suk, Lim, Joon Seok
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4489182/
https://www.ncbi.nlm.nih.gov/pubmed/26131558
http://dx.doi.org/10.1371/journal.pone.0131687
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author Kim, Sang Kyum
Kim, Honsoul
Koh, Gou Young
Lim, Dae-Sik
Yu, Dae-Yeul
Kim, Man Deuk
Park, Mi-Suk
Lim, Joon Seok
author_facet Kim, Sang Kyum
Kim, Honsoul
Koh, Gou Young
Lim, Dae-Sik
Yu, Dae-Yeul
Kim, Man Deuk
Park, Mi-Suk
Lim, Joon Seok
author_sort Kim, Sang Kyum
collection PubMed
description PURPOSE: Human hepatocellular carcinoma (HCC) has unique vascular features, which require selective imaging of hepatic arterial perfusion and portal venous perfusion with vascular catheterization for sufficient evaluation. Unlike in humans, vessels in mice are too small to catheterize, and the importance of separately imaging the feeding vessels of tumors is frequently overlooked in hepatic tumor models. The purpose of this study was to perform selective latex angiography in several mouse liver tumor models and assess their suitability. MATERIALS AND METHODS: In several ectopic (Lewis lung carcinoma, B16/F10 melanoma cell lines) and spontaneous liver tumor (Albumin-Cre/MST1(fl/fl)/MST2(fl/fl), Albumin-Cre/WW45(fl/fl), and H-ras12V genetically modified mouse) models, the heart left ventricle and/or main portal vein of mice was punctured, and latex dye was infused to achieve selective latex arteriography and/or portography. RESULTS: H-ras12V transgenic mice (a HCC and hepatic adenoma model) developed multiple liver nodules that displayed three different perfusion patterns (portal venous or hepatic artery perfusion predominant, mixed perfusion), indicating intra-tumoral vascular heterogeneity. Selective latex angiography revealed that the Lewis lung carcinoma implant model and the Albumin-Cre/WW45(fl/fl) model reproduced conventional angiography findings of human HCC. Specifically, these mice developed tumors with abundant feeding arteries but no portal venous perfusion. CONCLUSION: Different hepatic tumor models showed different tumor vessel characteristics that influence the suitability of the model and that should be considered when designing translational experiments. Selective latex angiography applied to certain mouse tumor models (both ectopic and spontaneous) closely simulated typical characteristics of human HCC vascular imaging.
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spelling pubmed-44891822015-07-14 Mouse Hepatic Tumor Vascular Imaging by Experimental Selective Angiography Kim, Sang Kyum Kim, Honsoul Koh, Gou Young Lim, Dae-Sik Yu, Dae-Yeul Kim, Man Deuk Park, Mi-Suk Lim, Joon Seok PLoS One Research Article PURPOSE: Human hepatocellular carcinoma (HCC) has unique vascular features, which require selective imaging of hepatic arterial perfusion and portal venous perfusion with vascular catheterization for sufficient evaluation. Unlike in humans, vessels in mice are too small to catheterize, and the importance of separately imaging the feeding vessels of tumors is frequently overlooked in hepatic tumor models. The purpose of this study was to perform selective latex angiography in several mouse liver tumor models and assess their suitability. MATERIALS AND METHODS: In several ectopic (Lewis lung carcinoma, B16/F10 melanoma cell lines) and spontaneous liver tumor (Albumin-Cre/MST1(fl/fl)/MST2(fl/fl), Albumin-Cre/WW45(fl/fl), and H-ras12V genetically modified mouse) models, the heart left ventricle and/or main portal vein of mice was punctured, and latex dye was infused to achieve selective latex arteriography and/or portography. RESULTS: H-ras12V transgenic mice (a HCC and hepatic adenoma model) developed multiple liver nodules that displayed three different perfusion patterns (portal venous or hepatic artery perfusion predominant, mixed perfusion), indicating intra-tumoral vascular heterogeneity. Selective latex angiography revealed that the Lewis lung carcinoma implant model and the Albumin-Cre/WW45(fl/fl) model reproduced conventional angiography findings of human HCC. Specifically, these mice developed tumors with abundant feeding arteries but no portal venous perfusion. CONCLUSION: Different hepatic tumor models showed different tumor vessel characteristics that influence the suitability of the model and that should be considered when designing translational experiments. Selective latex angiography applied to certain mouse tumor models (both ectopic and spontaneous) closely simulated typical characteristics of human HCC vascular imaging. Public Library of Science 2015-07-01 /pmc/articles/PMC4489182/ /pubmed/26131558 http://dx.doi.org/10.1371/journal.pone.0131687 Text en © 2015 Kim et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kim, Sang Kyum
Kim, Honsoul
Koh, Gou Young
Lim, Dae-Sik
Yu, Dae-Yeul
Kim, Man Deuk
Park, Mi-Suk
Lim, Joon Seok
Mouse Hepatic Tumor Vascular Imaging by Experimental Selective Angiography
title Mouse Hepatic Tumor Vascular Imaging by Experimental Selective Angiography
title_full Mouse Hepatic Tumor Vascular Imaging by Experimental Selective Angiography
title_fullStr Mouse Hepatic Tumor Vascular Imaging by Experimental Selective Angiography
title_full_unstemmed Mouse Hepatic Tumor Vascular Imaging by Experimental Selective Angiography
title_short Mouse Hepatic Tumor Vascular Imaging by Experimental Selective Angiography
title_sort mouse hepatic tumor vascular imaging by experimental selective angiography
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4489182/
https://www.ncbi.nlm.nih.gov/pubmed/26131558
http://dx.doi.org/10.1371/journal.pone.0131687
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