Response to Esteve-Gassent et al.: flaB sequences obtained from Texas PCR products are identical to the positive control strain Borrelia burgdorferi B31

Feria-Arroyo et al. had reported previously that, based on PCR analysis, 45 % of Ixodes scapularis ticks collected in Texas and Mexico were infected with the Lyme disease spirochete Borrelia burgdorferi (Parasit. Vectors 2014, 7:199). However, our analyses of their initial data (Parasit. Vectors 201...

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Autores principales: Norris, Steven J., Barbour, Alan G., Fish, Durland, Diuk-Wasser, Maria A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Letter to the Editor
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4489397/
https://www.ncbi.nlm.nih.gov/pubmed/26050617
http://dx.doi.org/10.1186/s13071-015-0899-x
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author Norris, Steven J.
Barbour, Alan G.
Fish, Durland
Diuk-Wasser, Maria A.
author_facet Norris, Steven J.
Barbour, Alan G.
Fish, Durland
Diuk-Wasser, Maria A.
author_sort Norris, Steven J.
collection PubMed
description Feria-Arroyo et al. had reported previously that, based on PCR analysis, 45 % of Ixodes scapularis ticks collected in Texas and Mexico were infected with the Lyme disease spirochete Borrelia burgdorferi (Parasit. Vectors 2014, 7:199). However, our analyses of their initial data (Parasit. Vectors 2014, 7:467) and a recent response by Esteve-Gassent et al. (Parasit. Vectors 2015, 8:129) provide evidence that the positive PCR results obtained from both ribosomal RNA intergenic sequences and the flagellin gene flaB are highly likely due to contamination by the B. burgdorferi B31 positive control strain.
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spelling pubmed-44893972015-07-03 Response to Esteve-Gassent et al.: flaB sequences obtained from Texas PCR products are identical to the positive control strain Borrelia burgdorferi B31 Norris, Steven J. Barbour, Alan G. Fish, Durland Diuk-Wasser, Maria A. Parasit Vectors Letter to the Editor Feria-Arroyo et al. had reported previously that, based on PCR analysis, 45 % of Ixodes scapularis ticks collected in Texas and Mexico were infected with the Lyme disease spirochete Borrelia burgdorferi (Parasit. Vectors 2014, 7:199). However, our analyses of their initial data (Parasit. Vectors 2014, 7:467) and a recent response by Esteve-Gassent et al. (Parasit. Vectors 2015, 8:129) provide evidence that the positive PCR results obtained from both ribosomal RNA intergenic sequences and the flagellin gene flaB are highly likely due to contamination by the B. burgdorferi B31 positive control strain. BioMed Central 2015-06-09 /pmc/articles/PMC4489397/ /pubmed/26050617 http://dx.doi.org/10.1186/s13071-015-0899-x Text en © Norris et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Letter to the Editor
Norris, Steven J.
Barbour, Alan G.
Fish, Durland
Diuk-Wasser, Maria A.
Response to Esteve-Gassent et al.: flaB sequences obtained from Texas PCR products are identical to the positive control strain Borrelia burgdorferi B31
title Response to Esteve-Gassent et al.: flaB sequences obtained from Texas PCR products are identical to the positive control strain Borrelia burgdorferi B31
title_full Response to Esteve-Gassent et al.: flaB sequences obtained from Texas PCR products are identical to the positive control strain Borrelia burgdorferi B31
title_fullStr Response to Esteve-Gassent et al.: flaB sequences obtained from Texas PCR products are identical to the positive control strain Borrelia burgdorferi B31
title_full_unstemmed Response to Esteve-Gassent et al.: flaB sequences obtained from Texas PCR products are identical to the positive control strain Borrelia burgdorferi B31
title_short Response to Esteve-Gassent et al.: flaB sequences obtained from Texas PCR products are identical to the positive control strain Borrelia burgdorferi B31
title_sort response to esteve-gassent et al.: flab sequences obtained from texas pcr products are identical to the positive control strain borrelia burgdorferi b31
topic Letter to the Editor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4489397/
https://www.ncbi.nlm.nih.gov/pubmed/26050617
http://dx.doi.org/10.1186/s13071-015-0899-x
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AT diukwassermariaa responsetoestevegassentetalflabsequencesobtainedfromtexaspcrproductsareidenticaltothepositivecontrolstrainborreliaburgdorferib31

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