Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods

This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloi...

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Autores principales: de Paula, Fabiana Martins, Malta, Fernanda de Mello, Marques, Priscilla Duarte, Sitta, Renata Barnabé, Pinho, João Renato Rebello, Gryschek, Ronaldo César Borges, Chieffi, Pedro Paulo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Instituto Oswaldo Cruz, Ministério da Saúde 2015
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4489462/
https://www.ncbi.nlm.nih.gov/pubmed/25946255
http://dx.doi.org/10.1590/0074-02760140371
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author de Paula, Fabiana Martins
Malta, Fernanda de Mello
Marques, Priscilla Duarte
Sitta, Renata Barnabé
Pinho, João Renato Rebello
Gryschek, Ronaldo César Borges
Chieffi, Pedro Paulo
author_facet de Paula, Fabiana Martins
Malta, Fernanda de Mello
Marques, Priscilla Duarte
Sitta, Renata Barnabé
Pinho, João Renato Rebello
Gryschek, Ronaldo César Borges
Chieffi, Pedro Paulo
author_sort de Paula, Fabiana Martins
collection PubMed
description This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR. The results show that molecular methods can be used as alternative tools for detecting S. stercoralis in human stool samples in tropical areas.
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spelling pubmed-44894622015-07-06 Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods de Paula, Fabiana Martins Malta, Fernanda de Mello Marques, Priscilla Duarte Sitta, Renata Barnabé Pinho, João Renato Rebello Gryschek, Ronaldo César Borges Chieffi, Pedro Paulo Mem Inst Oswaldo Cruz Technical Note This study aimed to evaluate the use of conventional polymerase chain reaction (cPCR) and real-time quantitative PCR (qPCR) in the diagnosis of human strongyloidiasis from stool samples in tropical areas. Stool samples were collected from individuals and were determined to be positive for Strongyloides stercoralis (group I), negative for S. stercoralis (group II) and positive for other enteroparasite species (group III). DNA specific to S. stercoralis was found in 76.7% of group I samples by cPCR and in 90% of group I samples by qPCR. The results show that molecular methods can be used as alternative tools for detecting S. stercoralis in human stool samples in tropical areas. Instituto Oswaldo Cruz, Ministério da Saúde 2015-04 /pmc/articles/PMC4489462/ /pubmed/25946255 http://dx.doi.org/10.1590/0074-02760140371 Text en http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Technical Note
de Paula, Fabiana Martins
Malta, Fernanda de Mello
Marques, Priscilla Duarte
Sitta, Renata Barnabé
Pinho, João Renato Rebello
Gryschek, Ronaldo César Borges
Chieffi, Pedro Paulo
Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods
title Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods
title_full Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods
title_fullStr Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods
title_full_unstemmed Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods
title_short Molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods
title_sort molecular diagnosis of strongyloidiasis in tropical areas: a comparison of conventional and real-time polymerase chain reaction with parasitological methods
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4489462/
https://www.ncbi.nlm.nih.gov/pubmed/25946255
http://dx.doi.org/10.1590/0074-02760140371
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