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Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing
The oral microbiome, which is closely associated with many diseases, and the resident pathogenic oral bacteria, which can be transferred by close physical contact, are important public health considerations. Although the dog is the most common companion animal, the composition of the canine oral mic...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4489859/ https://www.ncbi.nlm.nih.gov/pubmed/26134411 http://dx.doi.org/10.1371/journal.pone.0131468 |
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author | Oh, Changin Lee, Kunkyu Cheong, Yeotaek Lee, Sang-Won Park, Seung-Yong Song, Chang-Seon Choi, In-Soo Lee, Joong-Bok |
author_facet | Oh, Changin Lee, Kunkyu Cheong, Yeotaek Lee, Sang-Won Park, Seung-Yong Song, Chang-Seon Choi, In-Soo Lee, Joong-Bok |
author_sort | Oh, Changin |
collection | PubMed |
description | The oral microbiome, which is closely associated with many diseases, and the resident pathogenic oral bacteria, which can be transferred by close physical contact, are important public health considerations. Although the dog is the most common companion animal, the composition of the canine oral microbiome, which may include human pathogenic bacteria, and its relationship with that of their owners are unclear. In this study, 16S rDNA pyrosequencing was used to compare the oral microbiomes of 10 dogs and their owners and to identify zoonotic pathogens. Pyrosequencing revealed 246 operational taxonomic units in the 10 samples, representing 57 genera from eight bacterial phyla. Firmicutes (57.6%), Proteobacteria (21.6%), Bacteroidetes (9.8%), Actinobacteria (7.1%), and Fusobacteria (3.9%) were the predominant phyla in the human oral samples, whereas Proteobacteria (25.7%), Actinobacteria (21%), Bacteroidetes (19.7%), Firmicutes (19.3%), and Fusobacteria (12.3%) were predominant in the canine oral samples. The predominant genera in the human samples were Streptococcus (43.9%), Neisseria (10.3%), Haemophilus (9.6%), Prevotella (8.4%), and Veillonella (8.1%), whereas the predominant genera in the canine samples were Actinomyces (17.2%), Unknown (16.8), Porphyromonas (14.8), Fusobacterium (11.8), and Neisseria (7.2%). The oral microbiomes of dogs and their owners were appreciably different, and similarity in the microbiomes of canines and their owners was not correlated with residing in the same household. Oral-to-oral transfer of Neisseria shayeganii, Porphyromonas canigingivalis, Tannerella forsythia, and Streptococcus minor from dogs to humans was suspected. The finding of potentially zoonotic and periodontopathic bacteria in the canine oral microbiome may be a public health concern. |
format | Online Article Text |
id | pubmed-4489859 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44898592015-07-15 Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing Oh, Changin Lee, Kunkyu Cheong, Yeotaek Lee, Sang-Won Park, Seung-Yong Song, Chang-Seon Choi, In-Soo Lee, Joong-Bok PLoS One Research Article The oral microbiome, which is closely associated with many diseases, and the resident pathogenic oral bacteria, which can be transferred by close physical contact, are important public health considerations. Although the dog is the most common companion animal, the composition of the canine oral microbiome, which may include human pathogenic bacteria, and its relationship with that of their owners are unclear. In this study, 16S rDNA pyrosequencing was used to compare the oral microbiomes of 10 dogs and their owners and to identify zoonotic pathogens. Pyrosequencing revealed 246 operational taxonomic units in the 10 samples, representing 57 genera from eight bacterial phyla. Firmicutes (57.6%), Proteobacteria (21.6%), Bacteroidetes (9.8%), Actinobacteria (7.1%), and Fusobacteria (3.9%) were the predominant phyla in the human oral samples, whereas Proteobacteria (25.7%), Actinobacteria (21%), Bacteroidetes (19.7%), Firmicutes (19.3%), and Fusobacteria (12.3%) were predominant in the canine oral samples. The predominant genera in the human samples were Streptococcus (43.9%), Neisseria (10.3%), Haemophilus (9.6%), Prevotella (8.4%), and Veillonella (8.1%), whereas the predominant genera in the canine samples were Actinomyces (17.2%), Unknown (16.8), Porphyromonas (14.8), Fusobacterium (11.8), and Neisseria (7.2%). The oral microbiomes of dogs and their owners were appreciably different, and similarity in the microbiomes of canines and their owners was not correlated with residing in the same household. Oral-to-oral transfer of Neisseria shayeganii, Porphyromonas canigingivalis, Tannerella forsythia, and Streptococcus minor from dogs to humans was suspected. The finding of potentially zoonotic and periodontopathic bacteria in the canine oral microbiome may be a public health concern. Public Library of Science 2015-07-02 /pmc/articles/PMC4489859/ /pubmed/26134411 http://dx.doi.org/10.1371/journal.pone.0131468 Text en © 2015 Oh et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Oh, Changin Lee, Kunkyu Cheong, Yeotaek Lee, Sang-Won Park, Seung-Yong Song, Chang-Seon Choi, In-Soo Lee, Joong-Bok Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing |
title | Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing |
title_full | Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing |
title_fullStr | Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing |
title_full_unstemmed | Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing |
title_short | Comparison of the Oral Microbiomes of Canines and Their Owners Using Next-Generation Sequencing |
title_sort | comparison of the oral microbiomes of canines and their owners using next-generation sequencing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4489859/ https://www.ncbi.nlm.nih.gov/pubmed/26134411 http://dx.doi.org/10.1371/journal.pone.0131468 |
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