Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue

Adaptive optics by direct imaging of the wavefront distortions of a laser-induced guide star has long been used in astronomy, and more recently in microscopy to compensate for aberrations in transparent specimens. Here we extend this approach to tissues that strongly scatter visible light by exploit...

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Detalles Bibliográficos
Autores principales: Wang, Kai, Sun, Wenzhi, Richie, Christopher T., Harvey, Brandon K., Betzig, Eric, Ji, Na
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490402/
https://www.ncbi.nlm.nih.gov/pubmed/26073070
http://dx.doi.org/10.1038/ncomms8276
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author Wang, Kai
Sun, Wenzhi
Richie, Christopher T.
Harvey, Brandon K.
Betzig, Eric
Ji, Na
author_facet Wang, Kai
Sun, Wenzhi
Richie, Christopher T.
Harvey, Brandon K.
Betzig, Eric
Ji, Na
author_sort Wang, Kai
collection PubMed
description Adaptive optics by direct imaging of the wavefront distortions of a laser-induced guide star has long been used in astronomy, and more recently in microscopy to compensate for aberrations in transparent specimens. Here we extend this approach to tissues that strongly scatter visible light by exploiting the reduced scattering of near-infrared guide stars. The method enables in vivo two-photon morphological and functional imaging down to 700 μm inside the mouse brain.
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spelling pubmed-44904022015-07-13 Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue Wang, Kai Sun, Wenzhi Richie, Christopher T. Harvey, Brandon K. Betzig, Eric Ji, Na Nat Commun Article Adaptive optics by direct imaging of the wavefront distortions of a laser-induced guide star has long been used in astronomy, and more recently in microscopy to compensate for aberrations in transparent specimens. Here we extend this approach to tissues that strongly scatter visible light by exploiting the reduced scattering of near-infrared guide stars. The method enables in vivo two-photon morphological and functional imaging down to 700 μm inside the mouse brain. Nature Pub. Group 2015-06-15 /pmc/articles/PMC4490402/ /pubmed/26073070 http://dx.doi.org/10.1038/ncomms8276 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Wang, Kai
Sun, Wenzhi
Richie, Christopher T.
Harvey, Brandon K.
Betzig, Eric
Ji, Na
Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue
title Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue
title_full Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue
title_fullStr Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue
title_full_unstemmed Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue
title_short Direct wavefront sensing for high-resolution in vivo imaging in scattering tissue
title_sort direct wavefront sensing for high-resolution in vivo imaging in scattering tissue
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490402/
https://www.ncbi.nlm.nih.gov/pubmed/26073070
http://dx.doi.org/10.1038/ncomms8276
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