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Induction of Oxidative Stress and Antioxidative Mechanisms in Arabidopsis thaliana after Uranium Exposure at pH 7.5

To evaluate the environmental impact of uranium (U) contamination, it is important to investigate the effects of U at ecologically relevant conditions. Since U speciation, and hence its toxicity, strongly depends on environmental pH, the present study aimed to investigate dose-dependent effects of U...

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Autores principales: Saenen, Eline, Horemans, Nele, Vanhoudt, Nathalie, Vandenhove, Hildegarde, Biermans, Geert, Van Hees, May, Wannijn, Jean, Vangronsveld, Jaco, Cuypers, Ann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490451/
https://www.ncbi.nlm.nih.gov/pubmed/26042463
http://dx.doi.org/10.3390/ijms160612405
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author Saenen, Eline
Horemans, Nele
Vanhoudt, Nathalie
Vandenhove, Hildegarde
Biermans, Geert
Van Hees, May
Wannijn, Jean
Vangronsveld, Jaco
Cuypers, Ann
author_facet Saenen, Eline
Horemans, Nele
Vanhoudt, Nathalie
Vandenhove, Hildegarde
Biermans, Geert
Van Hees, May
Wannijn, Jean
Vangronsveld, Jaco
Cuypers, Ann
author_sort Saenen, Eline
collection PubMed
description To evaluate the environmental impact of uranium (U) contamination, it is important to investigate the effects of U at ecologically relevant conditions. Since U speciation, and hence its toxicity, strongly depends on environmental pH, the present study aimed to investigate dose-dependent effects of U at pH 7.5. Arabidopsis thaliana plants (Mouse-ear Cress) were exposed for three days to different U concentrations at pH 7.5. In the roots, the increased capacities of ascorbate peroxidase and glutathione reductase indicate an important role for the ascorbate-glutathione cycle during U-induced stress. However, a significant decrease in the ascorbate redox state was observed after exposure to 75 and 100 µM U, indicating that those roots are severely stressed. In accordance with the roots, the ascorbate-glutathione cycle plays an important role in the antioxidative defence systems in A. thaliana leaves exposed to U at pH 7.5 as the ascorbate and glutathione biosynthesis were upregulated. In addition, small inductions of enzymes of the antioxidative defence system were observed at lower U concentrations to counteract the U-induced stress. However, at higher U concentrations it seems that the antioxidative defence system of the leaves collapses as reductions in enzyme activities and gene expression levels were observed.
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spelling pubmed-44904512015-07-07 Induction of Oxidative Stress and Antioxidative Mechanisms in Arabidopsis thaliana after Uranium Exposure at pH 7.5 Saenen, Eline Horemans, Nele Vanhoudt, Nathalie Vandenhove, Hildegarde Biermans, Geert Van Hees, May Wannijn, Jean Vangronsveld, Jaco Cuypers, Ann Int J Mol Sci Article To evaluate the environmental impact of uranium (U) contamination, it is important to investigate the effects of U at ecologically relevant conditions. Since U speciation, and hence its toxicity, strongly depends on environmental pH, the present study aimed to investigate dose-dependent effects of U at pH 7.5. Arabidopsis thaliana plants (Mouse-ear Cress) were exposed for three days to different U concentrations at pH 7.5. In the roots, the increased capacities of ascorbate peroxidase and glutathione reductase indicate an important role for the ascorbate-glutathione cycle during U-induced stress. However, a significant decrease in the ascorbate redox state was observed after exposure to 75 and 100 µM U, indicating that those roots are severely stressed. In accordance with the roots, the ascorbate-glutathione cycle plays an important role in the antioxidative defence systems in A. thaliana leaves exposed to U at pH 7.5 as the ascorbate and glutathione biosynthesis were upregulated. In addition, small inductions of enzymes of the antioxidative defence system were observed at lower U concentrations to counteract the U-induced stress. However, at higher U concentrations it seems that the antioxidative defence system of the leaves collapses as reductions in enzyme activities and gene expression levels were observed. MDPI 2015-06-02 /pmc/articles/PMC4490451/ /pubmed/26042463 http://dx.doi.org/10.3390/ijms160612405 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Saenen, Eline
Horemans, Nele
Vanhoudt, Nathalie
Vandenhove, Hildegarde
Biermans, Geert
Van Hees, May
Wannijn, Jean
Vangronsveld, Jaco
Cuypers, Ann
Induction of Oxidative Stress and Antioxidative Mechanisms in Arabidopsis thaliana after Uranium Exposure at pH 7.5
title Induction of Oxidative Stress and Antioxidative Mechanisms in Arabidopsis thaliana after Uranium Exposure at pH 7.5
title_full Induction of Oxidative Stress and Antioxidative Mechanisms in Arabidopsis thaliana after Uranium Exposure at pH 7.5
title_fullStr Induction of Oxidative Stress and Antioxidative Mechanisms in Arabidopsis thaliana after Uranium Exposure at pH 7.5
title_full_unstemmed Induction of Oxidative Stress and Antioxidative Mechanisms in Arabidopsis thaliana after Uranium Exposure at pH 7.5
title_short Induction of Oxidative Stress and Antioxidative Mechanisms in Arabidopsis thaliana after Uranium Exposure at pH 7.5
title_sort induction of oxidative stress and antioxidative mechanisms in arabidopsis thaliana after uranium exposure at ph 7.5
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490451/
https://www.ncbi.nlm.nih.gov/pubmed/26042463
http://dx.doi.org/10.3390/ijms160612405
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