Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia

Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific t...

Descripción completa

Detalles Bibliográficos
Autores principales: Souders, Colby A., Maynard, Sharon E., Yan, Jing, Wang, Yang, Boatright, Naomi K., Sedan, Jessica, Balyozian, David, Cheslock, Peter S., Molrine, Deborah C., Moore Simas, Tiffany A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490453/
https://www.ncbi.nlm.nih.gov/pubmed/26042465
http://dx.doi.org/10.3390/ijms160612436
_version_ 1782379507000279040
author Souders, Colby A.
Maynard, Sharon E.
Yan, Jing
Wang, Yang
Boatright, Naomi K.
Sedan, Jessica
Balyozian, David
Cheslock, Peter S.
Molrine, Deborah C.
Moore Simas, Tiffany A.
author_facet Souders, Colby A.
Maynard, Sharon E.
Yan, Jing
Wang, Yang
Boatright, Naomi K.
Sedan, Jessica
Balyozian, David
Cheslock, Peter S.
Molrine, Deborah C.
Moore Simas, Tiffany A.
author_sort Souders, Colby A.
collection PubMed
description Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized.
format Online
Article
Text
id pubmed-4490453
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-44904532015-07-07 Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia Souders, Colby A. Maynard, Sharon E. Yan, Jing Wang, Yang Boatright, Naomi K. Sedan, Jessica Balyozian, David Cheslock, Peter S. Molrine, Deborah C. Moore Simas, Tiffany A. Int J Mol Sci Article Angiogenic biomarkers, including soluble fms-like tyrosine kinase 1 (sFlt1), are thought to be predictors of preeclampsia onset; however, improvement is needed before a widespread diagnostic test can be utilized. Here we describe the development and use of diagnostic monoclonal antibodies specific to the two main splice variants of sFlt1, sFlt1-1 and sFlt1-14. These antibodies were selected for their sensitivity and specificity to their respective sFlt1 isoform in a capture ELISA format. Data from this pilot study suggest that sFlt1-1 may be more predictive of preeclampsia than total sFlt1. It may be possible to improve current diagnostic platforms if more specific antibodies are utilized. MDPI 2015-06-02 /pmc/articles/PMC4490453/ /pubmed/26042465 http://dx.doi.org/10.3390/ijms160612436 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Souders, Colby A.
Maynard, Sharon E.
Yan, Jing
Wang, Yang
Boatright, Naomi K.
Sedan, Jessica
Balyozian, David
Cheslock, Peter S.
Molrine, Deborah C.
Moore Simas, Tiffany A.
Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia
title Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia
title_full Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia
title_fullStr Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia
title_full_unstemmed Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia
title_short Circulating Levels of sFlt1 Splice Variants as Predictive Markers for the Development of Preeclampsia
title_sort circulating levels of sflt1 splice variants as predictive markers for the development of preeclampsia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490453/
https://www.ncbi.nlm.nih.gov/pubmed/26042465
http://dx.doi.org/10.3390/ijms160612436
work_keys_str_mv AT souderscolbya circulatinglevelsofsflt1splicevariantsaspredictivemarkersforthedevelopmentofpreeclampsia
AT maynardsharone circulatinglevelsofsflt1splicevariantsaspredictivemarkersforthedevelopmentofpreeclampsia
AT yanjing circulatinglevelsofsflt1splicevariantsaspredictivemarkersforthedevelopmentofpreeclampsia
AT wangyang circulatinglevelsofsflt1splicevariantsaspredictivemarkersforthedevelopmentofpreeclampsia
AT boatrightnaomik circulatinglevelsofsflt1splicevariantsaspredictivemarkersforthedevelopmentofpreeclampsia
AT sedanjessica circulatinglevelsofsflt1splicevariantsaspredictivemarkersforthedevelopmentofpreeclampsia
AT balyoziandavid circulatinglevelsofsflt1splicevariantsaspredictivemarkersforthedevelopmentofpreeclampsia
AT cheslockpeters circulatinglevelsofsflt1splicevariantsaspredictivemarkersforthedevelopmentofpreeclampsia
AT molrinedeborahc circulatinglevelsofsflt1splicevariantsaspredictivemarkersforthedevelopmentofpreeclampsia
AT mooresimastiffanya circulatinglevelsofsflt1splicevariantsaspredictivemarkersforthedevelopmentofpreeclampsia

Ejemplares similares