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Engraftment of Prevascularized, Tissue Engineered Constructs in a Novel Rabbit Segmental Bone Defect Model

The gold standard treatment of large segmental bone defects is autologous bone transfer, which suffers from low availability and additional morbidity. Tissue engineered bone able to engraft orthotopically and a suitable animal model for pre-clinical testing are direly needed. This study aimed to eva...

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Autores principales: Kaempfen, Alexandre, Todorov, Atanas, Güven, Sinan, Largo, René D., Jaquiéry, Claude, Scherberich, Arnaud, Martin, Ivan, Schaefer, Dirk J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490464/
https://www.ncbi.nlm.nih.gov/pubmed/26053395
http://dx.doi.org/10.3390/ijms160612616
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author Kaempfen, Alexandre
Todorov, Atanas
Güven, Sinan
Largo, René D.
Jaquiéry, Claude
Scherberich, Arnaud
Martin, Ivan
Schaefer, Dirk J.
author_facet Kaempfen, Alexandre
Todorov, Atanas
Güven, Sinan
Largo, René D.
Jaquiéry, Claude
Scherberich, Arnaud
Martin, Ivan
Schaefer, Dirk J.
author_sort Kaempfen, Alexandre
collection PubMed
description The gold standard treatment of large segmental bone defects is autologous bone transfer, which suffers from low availability and additional morbidity. Tissue engineered bone able to engraft orthotopically and a suitable animal model for pre-clinical testing are direly needed. This study aimed to evaluate engraftment of tissue-engineered bone with different prevascularization strategies in a novel segmental defect model in the rabbit humerus. Decellularized bone matrix (Tutobone) seeded with bone marrow mesenchymal stromal cells was used directly orthotopically or combined with a vessel and inserted immediately (1-step) or only after six weeks of subcutaneous “incubation” (2-step). After 12 weeks, histological and radiological assessment was performed. Variable callus formation was observed. No bone formation or remodeling of the graft through TRAP positive osteoclasts could be detected. Instead, a variable amount of necrotic tissue formed. Although necrotic area correlated significantly with amount of vessels and the 2-step strategy had significantly more vessels than the 1-step strategy, no significant reduction of necrotic area was found. In conclusion, the animal model developed here represents a highly challenging situation, for which a suitable engineered bone graft with better prevascularization, better resorbability and higher osteogenicity has yet to be developed.
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spelling pubmed-44904642015-07-07 Engraftment of Prevascularized, Tissue Engineered Constructs in a Novel Rabbit Segmental Bone Defect Model Kaempfen, Alexandre Todorov, Atanas Güven, Sinan Largo, René D. Jaquiéry, Claude Scherberich, Arnaud Martin, Ivan Schaefer, Dirk J. Int J Mol Sci Article The gold standard treatment of large segmental bone defects is autologous bone transfer, which suffers from low availability and additional morbidity. Tissue engineered bone able to engraft orthotopically and a suitable animal model for pre-clinical testing are direly needed. This study aimed to evaluate engraftment of tissue-engineered bone with different prevascularization strategies in a novel segmental defect model in the rabbit humerus. Decellularized bone matrix (Tutobone) seeded with bone marrow mesenchymal stromal cells was used directly orthotopically or combined with a vessel and inserted immediately (1-step) or only after six weeks of subcutaneous “incubation” (2-step). After 12 weeks, histological and radiological assessment was performed. Variable callus formation was observed. No bone formation or remodeling of the graft through TRAP positive osteoclasts could be detected. Instead, a variable amount of necrotic tissue formed. Although necrotic area correlated significantly with amount of vessels and the 2-step strategy had significantly more vessels than the 1-step strategy, no significant reduction of necrotic area was found. In conclusion, the animal model developed here represents a highly challenging situation, for which a suitable engineered bone graft with better prevascularization, better resorbability and higher osteogenicity has yet to be developed. MDPI 2015-06-04 /pmc/articles/PMC4490464/ /pubmed/26053395 http://dx.doi.org/10.3390/ijms160612616 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kaempfen, Alexandre
Todorov, Atanas
Güven, Sinan
Largo, René D.
Jaquiéry, Claude
Scherberich, Arnaud
Martin, Ivan
Schaefer, Dirk J.
Engraftment of Prevascularized, Tissue Engineered Constructs in a Novel Rabbit Segmental Bone Defect Model
title Engraftment of Prevascularized, Tissue Engineered Constructs in a Novel Rabbit Segmental Bone Defect Model
title_full Engraftment of Prevascularized, Tissue Engineered Constructs in a Novel Rabbit Segmental Bone Defect Model
title_fullStr Engraftment of Prevascularized, Tissue Engineered Constructs in a Novel Rabbit Segmental Bone Defect Model
title_full_unstemmed Engraftment of Prevascularized, Tissue Engineered Constructs in a Novel Rabbit Segmental Bone Defect Model
title_short Engraftment of Prevascularized, Tissue Engineered Constructs in a Novel Rabbit Segmental Bone Defect Model
title_sort engraftment of prevascularized, tissue engineered constructs in a novel rabbit segmental bone defect model
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490464/
https://www.ncbi.nlm.nih.gov/pubmed/26053395
http://dx.doi.org/10.3390/ijms160612616
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