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Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma
The present study aimed to identify serum biomarkers for the detection of hepatoblastoma (HB). Serum samples were collected from 71 HB patients (stage I, n = 19; stage II, n = 19, stage III, n = 19; and stage IV, n = 14) and 23 age- and sex-matched healthy children. Differential expression of serum...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490467/ https://www.ncbi.nlm.nih.gov/pubmed/26053398 http://dx.doi.org/10.3390/ijms160612669 |
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author | Zhao, Wei Li, Juan Zhang, Junjie Gao, Pengfei Pei, Hang Wang, Lei Guo, Fei Yu, Jiekai Zheng, Shu Wang, Jiaxiang |
author_facet | Zhao, Wei Li, Juan Zhang, Junjie Gao, Pengfei Pei, Hang Wang, Lei Guo, Fei Yu, Jiekai Zheng, Shu Wang, Jiaxiang |
author_sort | Zhao, Wei |
collection | PubMed |
description | The present study aimed to identify serum biomarkers for the detection of hepatoblastoma (HB). Serum samples were collected from 71 HB patients (stage I, n = 19; stage II, n = 19, stage III, n = 19; and stage IV, n = 14) and 23 age- and sex-matched healthy children. Differential expression of serum protein markers were screened using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS), and the target proteins were isolated and purified using HPLC and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), SEQUEST, and bioinformatics analysis. Differential protein expression was confirmed by enzyme-linked immunosorbent analysis (ELISA). SELDI-TOF-MS screening identified a differentially expressed protein with an m/z of 9348 Da, which was subsequently identified as Apo A–I; its expression was significantly lower in the HB group as compared to the normal control group (1546.67 ± 757.81 vs. 3359.21 ± 999.36, respectively; p < 0.01). Although the expression level decreased with increasing disease stage, pair-wise comparison revealed significant differences in Apo A–I expression between the normal group and the HB subgroups (p < 0.01). ELISA verified the reduced expression of Apo A–I in the HB group. Taken together, these results suggest that Apo A–I may represent a serum protein biomarker of HB. Further studies will assess the value of using Apo A–I expression for HB diagnosis and staging. |
format | Online Article Text |
id | pubmed-4490467 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-44904672015-07-07 Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma Zhao, Wei Li, Juan Zhang, Junjie Gao, Pengfei Pei, Hang Wang, Lei Guo, Fei Yu, Jiekai Zheng, Shu Wang, Jiaxiang Int J Mol Sci Article The present study aimed to identify serum biomarkers for the detection of hepatoblastoma (HB). Serum samples were collected from 71 HB patients (stage I, n = 19; stage II, n = 19, stage III, n = 19; and stage IV, n = 14) and 23 age- and sex-matched healthy children. Differential expression of serum protein markers were screened using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS), and the target proteins were isolated and purified using HPLC and identified using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS), SEQUEST, and bioinformatics analysis. Differential protein expression was confirmed by enzyme-linked immunosorbent analysis (ELISA). SELDI-TOF-MS screening identified a differentially expressed protein with an m/z of 9348 Da, which was subsequently identified as Apo A–I; its expression was significantly lower in the HB group as compared to the normal control group (1546.67 ± 757.81 vs. 3359.21 ± 999.36, respectively; p < 0.01). Although the expression level decreased with increasing disease stage, pair-wise comparison revealed significant differences in Apo A–I expression between the normal group and the HB subgroups (p < 0.01). ELISA verified the reduced expression of Apo A–I in the HB group. Taken together, these results suggest that Apo A–I may represent a serum protein biomarker of HB. Further studies will assess the value of using Apo A–I expression for HB diagnosis and staging. MDPI 2015-06-04 /pmc/articles/PMC4490467/ /pubmed/26053398 http://dx.doi.org/10.3390/ijms160612669 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zhao, Wei Li, Juan Zhang, Junjie Gao, Pengfei Pei, Hang Wang, Lei Guo, Fei Yu, Jiekai Zheng, Shu Wang, Jiaxiang Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma |
title | Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma |
title_full | Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma |
title_fullStr | Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma |
title_full_unstemmed | Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma |
title_short | Detection of Serum Protein Biomarkers for the Diagnosis and Staging of Hepatoblastoma |
title_sort | detection of serum protein biomarkers for the diagnosis and staging of hepatoblastoma |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490467/ https://www.ncbi.nlm.nih.gov/pubmed/26053398 http://dx.doi.org/10.3390/ijms160612669 |
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