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Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species

Infections related to Aspergillus species have emerged to become an important focus in infectious diseases, as a result of the increasing use of immunosuppressive agents and high fatality associated with invasive aspergillosis. However, laboratory diagnosis of Aspergillus infections remains difficul...

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Autores principales: Lee, Kim-Chung, Tam, Emily W. T., Lo, Ka-Ching, Tsang, Alan K. L., Lau, Candy C. Y., To, Kelvin K. W., Chan, Jasper F. W., Lam, Ching-Wan, Yuen, Kwok-Yung, Lau, Susanna K. P., Woo, Patrick C. Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490527/
https://www.ncbi.nlm.nih.gov/pubmed/26090713
http://dx.doi.org/10.3390/ijms160613850
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author Lee, Kim-Chung
Tam, Emily W. T.
Lo, Ka-Ching
Tsang, Alan K. L.
Lau, Candy C. Y.
To, Kelvin K. W.
Chan, Jasper F. W.
Lam, Ching-Wan
Yuen, Kwok-Yung
Lau, Susanna K. P.
Woo, Patrick C. Y.
author_facet Lee, Kim-Chung
Tam, Emily W. T.
Lo, Ka-Ching
Tsang, Alan K. L.
Lau, Candy C. Y.
To, Kelvin K. W.
Chan, Jasper F. W.
Lam, Ching-Wan
Yuen, Kwok-Yung
Lau, Susanna K. P.
Woo, Patrick C. Y.
author_sort Lee, Kim-Chung
collection PubMed
description Infections related to Aspergillus species have emerged to become an important focus in infectious diseases, as a result of the increasing use of immunosuppressive agents and high fatality associated with invasive aspergillosis. However, laboratory diagnosis of Aspergillus infections remains difficult. In this study, by comparing the metabolomic profiles of the culture supernatants of 30 strains of six pathogenic Aspergillus species (A. fumigatus, A. flavus, A. niger, A. terreus, A. nomius and A. tamarii) and 31 strains of 10 non-Aspergillus fungi, eight compounds present in all strains of the six Aspergillus species but not in any strain of the non-Aspergillus fungi were observed. One of the eight compounds, Leu–Glu–Leu–Glu, is a novel tetrapeptide and represents the first linear tetrapeptide observed in Aspergillus species, which we propose to be named aspergitide. Two other closely related Aspergillus-specific compounds, hydroxy-(sulfooxy)benzoic acid and (sulfooxy)benzoic acid, may possess anti-inflammatory properties, as 2-(sulfooxy)benzoic acid possesses a structure similar to those of aspirin [2-(acetoxy)benzoic acid] and salicylic acid (2-hydroxybenzoic acid). Further studies to examine the potentials of these Aspergillus-specific compounds for laboratory diagnosis of aspergillosis are warranted and further experiments will reveal whether Leu–Glu–Leu–Glu, hydroxy-(sulfooxy)benzoic acid and (sulfooxy)benzoic acid are virulent factors of the pathogenic Aspergillus species.
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spelling pubmed-44905272015-07-07 Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species Lee, Kim-Chung Tam, Emily W. T. Lo, Ka-Ching Tsang, Alan K. L. Lau, Candy C. Y. To, Kelvin K. W. Chan, Jasper F. W. Lam, Ching-Wan Yuen, Kwok-Yung Lau, Susanna K. P. Woo, Patrick C. Y. Int J Mol Sci Article Infections related to Aspergillus species have emerged to become an important focus in infectious diseases, as a result of the increasing use of immunosuppressive agents and high fatality associated with invasive aspergillosis. However, laboratory diagnosis of Aspergillus infections remains difficult. In this study, by comparing the metabolomic profiles of the culture supernatants of 30 strains of six pathogenic Aspergillus species (A. fumigatus, A. flavus, A. niger, A. terreus, A. nomius and A. tamarii) and 31 strains of 10 non-Aspergillus fungi, eight compounds present in all strains of the six Aspergillus species but not in any strain of the non-Aspergillus fungi were observed. One of the eight compounds, Leu–Glu–Leu–Glu, is a novel tetrapeptide and represents the first linear tetrapeptide observed in Aspergillus species, which we propose to be named aspergitide. Two other closely related Aspergillus-specific compounds, hydroxy-(sulfooxy)benzoic acid and (sulfooxy)benzoic acid, may possess anti-inflammatory properties, as 2-(sulfooxy)benzoic acid possesses a structure similar to those of aspirin [2-(acetoxy)benzoic acid] and salicylic acid (2-hydroxybenzoic acid). Further studies to examine the potentials of these Aspergillus-specific compounds for laboratory diagnosis of aspergillosis are warranted and further experiments will reveal whether Leu–Glu–Leu–Glu, hydroxy-(sulfooxy)benzoic acid and (sulfooxy)benzoic acid are virulent factors of the pathogenic Aspergillus species. MDPI 2015-06-17 /pmc/articles/PMC4490527/ /pubmed/26090713 http://dx.doi.org/10.3390/ijms160613850 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Kim-Chung
Tam, Emily W. T.
Lo, Ka-Ching
Tsang, Alan K. L.
Lau, Candy C. Y.
To, Kelvin K. W.
Chan, Jasper F. W.
Lam, Ching-Wan
Yuen, Kwok-Yung
Lau, Susanna K. P.
Woo, Patrick C. Y.
Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species
title Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species
title_full Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species
title_fullStr Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species
title_full_unstemmed Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species
title_short Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species
title_sort metabolomics analysis reveals specific novel tetrapeptide and potential anti-inflammatory metabolites in pathogenic aspergillus species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490527/
https://www.ncbi.nlm.nih.gov/pubmed/26090713
http://dx.doi.org/10.3390/ijms160613850
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