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Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species
Infections related to Aspergillus species have emerged to become an important focus in infectious diseases, as a result of the increasing use of immunosuppressive agents and high fatality associated with invasive aspergillosis. However, laboratory diagnosis of Aspergillus infections remains difficul...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490527/ https://www.ncbi.nlm.nih.gov/pubmed/26090713 http://dx.doi.org/10.3390/ijms160613850 |
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author | Lee, Kim-Chung Tam, Emily W. T. Lo, Ka-Ching Tsang, Alan K. L. Lau, Candy C. Y. To, Kelvin K. W. Chan, Jasper F. W. Lam, Ching-Wan Yuen, Kwok-Yung Lau, Susanna K. P. Woo, Patrick C. Y. |
author_facet | Lee, Kim-Chung Tam, Emily W. T. Lo, Ka-Ching Tsang, Alan K. L. Lau, Candy C. Y. To, Kelvin K. W. Chan, Jasper F. W. Lam, Ching-Wan Yuen, Kwok-Yung Lau, Susanna K. P. Woo, Patrick C. Y. |
author_sort | Lee, Kim-Chung |
collection | PubMed |
description | Infections related to Aspergillus species have emerged to become an important focus in infectious diseases, as a result of the increasing use of immunosuppressive agents and high fatality associated with invasive aspergillosis. However, laboratory diagnosis of Aspergillus infections remains difficult. In this study, by comparing the metabolomic profiles of the culture supernatants of 30 strains of six pathogenic Aspergillus species (A. fumigatus, A. flavus, A. niger, A. terreus, A. nomius and A. tamarii) and 31 strains of 10 non-Aspergillus fungi, eight compounds present in all strains of the six Aspergillus species but not in any strain of the non-Aspergillus fungi were observed. One of the eight compounds, Leu–Glu–Leu–Glu, is a novel tetrapeptide and represents the first linear tetrapeptide observed in Aspergillus species, which we propose to be named aspergitide. Two other closely related Aspergillus-specific compounds, hydroxy-(sulfooxy)benzoic acid and (sulfooxy)benzoic acid, may possess anti-inflammatory properties, as 2-(sulfooxy)benzoic acid possesses a structure similar to those of aspirin [2-(acetoxy)benzoic acid] and salicylic acid (2-hydroxybenzoic acid). Further studies to examine the potentials of these Aspergillus-specific compounds for laboratory diagnosis of aspergillosis are warranted and further experiments will reveal whether Leu–Glu–Leu–Glu, hydroxy-(sulfooxy)benzoic acid and (sulfooxy)benzoic acid are virulent factors of the pathogenic Aspergillus species. |
format | Online Article Text |
id | pubmed-4490527 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-44905272015-07-07 Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species Lee, Kim-Chung Tam, Emily W. T. Lo, Ka-Ching Tsang, Alan K. L. Lau, Candy C. Y. To, Kelvin K. W. Chan, Jasper F. W. Lam, Ching-Wan Yuen, Kwok-Yung Lau, Susanna K. P. Woo, Patrick C. Y. Int J Mol Sci Article Infections related to Aspergillus species have emerged to become an important focus in infectious diseases, as a result of the increasing use of immunosuppressive agents and high fatality associated with invasive aspergillosis. However, laboratory diagnosis of Aspergillus infections remains difficult. In this study, by comparing the metabolomic profiles of the culture supernatants of 30 strains of six pathogenic Aspergillus species (A. fumigatus, A. flavus, A. niger, A. terreus, A. nomius and A. tamarii) and 31 strains of 10 non-Aspergillus fungi, eight compounds present in all strains of the six Aspergillus species but not in any strain of the non-Aspergillus fungi were observed. One of the eight compounds, Leu–Glu–Leu–Glu, is a novel tetrapeptide and represents the first linear tetrapeptide observed in Aspergillus species, which we propose to be named aspergitide. Two other closely related Aspergillus-specific compounds, hydroxy-(sulfooxy)benzoic acid and (sulfooxy)benzoic acid, may possess anti-inflammatory properties, as 2-(sulfooxy)benzoic acid possesses a structure similar to those of aspirin [2-(acetoxy)benzoic acid] and salicylic acid (2-hydroxybenzoic acid). Further studies to examine the potentials of these Aspergillus-specific compounds for laboratory diagnosis of aspergillosis are warranted and further experiments will reveal whether Leu–Glu–Leu–Glu, hydroxy-(sulfooxy)benzoic acid and (sulfooxy)benzoic acid are virulent factors of the pathogenic Aspergillus species. MDPI 2015-06-17 /pmc/articles/PMC4490527/ /pubmed/26090713 http://dx.doi.org/10.3390/ijms160613850 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lee, Kim-Chung Tam, Emily W. T. Lo, Ka-Ching Tsang, Alan K. L. Lau, Candy C. Y. To, Kelvin K. W. Chan, Jasper F. W. Lam, Ching-Wan Yuen, Kwok-Yung Lau, Susanna K. P. Woo, Patrick C. Y. Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species |
title | Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species |
title_full | Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species |
title_fullStr | Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species |
title_full_unstemmed | Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species |
title_short | Metabolomics Analysis Reveals Specific Novel Tetrapeptide and Potential Anti-Inflammatory Metabolites in Pathogenic Aspergillus species |
title_sort | metabolomics analysis reveals specific novel tetrapeptide and potential anti-inflammatory metabolites in pathogenic aspergillus species |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490527/ https://www.ncbi.nlm.nih.gov/pubmed/26090713 http://dx.doi.org/10.3390/ijms160613850 |
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