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Lipidomics by Supercritical Fluid Chromatography

This review enlightens the role of supercritical fluid chromatography (SFC) in the field of lipid analysis. SFC has been popular in the late 1980s and 1990s before almost disappearing due to the commercial success of liquid chromatography (LC). It is only 20 years later that a regain of interest app...

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Detalles Bibliográficos
Autores principales: Laboureur, Laurent, Ollero, Mario, Touboul, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490528/
https://www.ncbi.nlm.nih.gov/pubmed/26090714
http://dx.doi.org/10.3390/ijms160613868
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author Laboureur, Laurent
Ollero, Mario
Touboul, David
author_facet Laboureur, Laurent
Ollero, Mario
Touboul, David
author_sort Laboureur, Laurent
collection PubMed
description This review enlightens the role of supercritical fluid chromatography (SFC) in the field of lipid analysis. SFC has been popular in the late 1980s and 1990s before almost disappearing due to the commercial success of liquid chromatography (LC). It is only 20 years later that a regain of interest appeared when new commercial instruments were introduced. As SFC is fully compatible with the injection of extracts in pure organic solvent, this technique is perfectly suitable for lipid analysis and can be coupled with either highly universal (UV or evaporative light scattering) or highly specific (mass spectrometry) detection methods. A short history of the use of supercritical fluids as mobile phase for the separation oflipids will be introduced first. Then, the advantages and drawbacks of SFC are discussed for each class of lipids (fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterols, prenols, polyketides) defined by the LIPID MAPS consortium.
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spelling pubmed-44905282015-07-07 Lipidomics by Supercritical Fluid Chromatography Laboureur, Laurent Ollero, Mario Touboul, David Int J Mol Sci Review This review enlightens the role of supercritical fluid chromatography (SFC) in the field of lipid analysis. SFC has been popular in the late 1980s and 1990s before almost disappearing due to the commercial success of liquid chromatography (LC). It is only 20 years later that a regain of interest appeared when new commercial instruments were introduced. As SFC is fully compatible with the injection of extracts in pure organic solvent, this technique is perfectly suitable for lipid analysis and can be coupled with either highly universal (UV or evaporative light scattering) or highly specific (mass spectrometry) detection methods. A short history of the use of supercritical fluids as mobile phase for the separation oflipids will be introduced first. Then, the advantages and drawbacks of SFC are discussed for each class of lipids (fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterols, prenols, polyketides) defined by the LIPID MAPS consortium. MDPI 2015-06-17 /pmc/articles/PMC4490528/ /pubmed/26090714 http://dx.doi.org/10.3390/ijms160613868 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Laboureur, Laurent
Ollero, Mario
Touboul, David
Lipidomics by Supercritical Fluid Chromatography
title Lipidomics by Supercritical Fluid Chromatography
title_full Lipidomics by Supercritical Fluid Chromatography
title_fullStr Lipidomics by Supercritical Fluid Chromatography
title_full_unstemmed Lipidomics by Supercritical Fluid Chromatography
title_short Lipidomics by Supercritical Fluid Chromatography
title_sort lipidomics by supercritical fluid chromatography
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4490528/
https://www.ncbi.nlm.nih.gov/pubmed/26090714
http://dx.doi.org/10.3390/ijms160613868
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