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Glycan complexity dictates microbial resource allocation in the large intestine

The structure of the human gut microbiota is controlled primarily through the degradation of complex dietary carbohydrates, but the extent to which carbohydrate breakdown products are shared between members of the microbiota is unclear. We show here, using xylan as a model, that sharing the breakdow...

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Autores principales: Rogowski, Artur, Briggs, Jonathon A., Mortimer, Jennifer C., Tryfona, Theodora, Terrapon, Nicolas, Lowe, Elisabeth C., Baslé, Arnaud, Morland, Carl, Day, Alison M., Zheng, Hongjun, Rogers, Theresa E., Thompson, Paul, Hawkins, Alastair R., Yadav, Madhav P., Henrissat, Bernard, Martens, Eric C., Dupree, Paul, Gilbert, Harry J., Bolam, David N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Pub. Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491172/
https://www.ncbi.nlm.nih.gov/pubmed/26112186
http://dx.doi.org/10.1038/ncomms8481
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author Rogowski, Artur
Briggs, Jonathon A.
Mortimer, Jennifer C.
Tryfona, Theodora
Terrapon, Nicolas
Lowe, Elisabeth C.
Baslé, Arnaud
Morland, Carl
Day, Alison M.
Zheng, Hongjun
Rogers, Theresa E.
Thompson, Paul
Hawkins, Alastair R.
Yadav, Madhav P.
Henrissat, Bernard
Martens, Eric C.
Dupree, Paul
Gilbert, Harry J.
Bolam, David N.
author_facet Rogowski, Artur
Briggs, Jonathon A.
Mortimer, Jennifer C.
Tryfona, Theodora
Terrapon, Nicolas
Lowe, Elisabeth C.
Baslé, Arnaud
Morland, Carl
Day, Alison M.
Zheng, Hongjun
Rogers, Theresa E.
Thompson, Paul
Hawkins, Alastair R.
Yadav, Madhav P.
Henrissat, Bernard
Martens, Eric C.
Dupree, Paul
Gilbert, Harry J.
Bolam, David N.
author_sort Rogowski, Artur
collection PubMed
description The structure of the human gut microbiota is controlled primarily through the degradation of complex dietary carbohydrates, but the extent to which carbohydrate breakdown products are shared between members of the microbiota is unclear. We show here, using xylan as a model, that sharing the breakdown products of complex carbohydrates by key members of the microbiota, such as Bacteroides ovatus, is dependent on the complexity of the target glycan. Characterization of the extensive xylan degrading apparatus expressed by B. ovatus reveals that the breakdown of the polysaccharide by the human gut microbiota is significantly more complex than previous models suggested, which were based on the deconstruction of xylans containing limited monosaccharide side chains. Our report presents a highly complex and dynamic xylan degrading apparatus that is fine-tuned to recognize the different forms of the polysaccharide presented to the human gut microbiota.
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spelling pubmed-44911722015-07-08 Glycan complexity dictates microbial resource allocation in the large intestine Rogowski, Artur Briggs, Jonathon A. Mortimer, Jennifer C. Tryfona, Theodora Terrapon, Nicolas Lowe, Elisabeth C. Baslé, Arnaud Morland, Carl Day, Alison M. Zheng, Hongjun Rogers, Theresa E. Thompson, Paul Hawkins, Alastair R. Yadav, Madhav P. Henrissat, Bernard Martens, Eric C. Dupree, Paul Gilbert, Harry J. Bolam, David N. Nat Commun Article The structure of the human gut microbiota is controlled primarily through the degradation of complex dietary carbohydrates, but the extent to which carbohydrate breakdown products are shared between members of the microbiota is unclear. We show here, using xylan as a model, that sharing the breakdown products of complex carbohydrates by key members of the microbiota, such as Bacteroides ovatus, is dependent on the complexity of the target glycan. Characterization of the extensive xylan degrading apparatus expressed by B. ovatus reveals that the breakdown of the polysaccharide by the human gut microbiota is significantly more complex than previous models suggested, which were based on the deconstruction of xylans containing limited monosaccharide side chains. Our report presents a highly complex and dynamic xylan degrading apparatus that is fine-tuned to recognize the different forms of the polysaccharide presented to the human gut microbiota. Nature Pub. Group 2015-06-26 /pmc/articles/PMC4491172/ /pubmed/26112186 http://dx.doi.org/10.1038/ncomms8481 Text en Copyright © 2015, Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved. http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Rogowski, Artur
Briggs, Jonathon A.
Mortimer, Jennifer C.
Tryfona, Theodora
Terrapon, Nicolas
Lowe, Elisabeth C.
Baslé, Arnaud
Morland, Carl
Day, Alison M.
Zheng, Hongjun
Rogers, Theresa E.
Thompson, Paul
Hawkins, Alastair R.
Yadav, Madhav P.
Henrissat, Bernard
Martens, Eric C.
Dupree, Paul
Gilbert, Harry J.
Bolam, David N.
Glycan complexity dictates microbial resource allocation in the large intestine
title Glycan complexity dictates microbial resource allocation in the large intestine
title_full Glycan complexity dictates microbial resource allocation in the large intestine
title_fullStr Glycan complexity dictates microbial resource allocation in the large intestine
title_full_unstemmed Glycan complexity dictates microbial resource allocation in the large intestine
title_short Glycan complexity dictates microbial resource allocation in the large intestine
title_sort glycan complexity dictates microbial resource allocation in the large intestine
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491172/
https://www.ncbi.nlm.nih.gov/pubmed/26112186
http://dx.doi.org/10.1038/ncomms8481
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