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Actinospica durhamensis sp. nov., isolated from a spruce forest soil
Seven acidophilic actinobacteria isolated from humus and mineral layers of a spruce forest soil were examined using a polyphasic approach. Chemotaxonomic properties of the isolates were found to be consistent with their classification in the genus Actinospica. The strains formed a distinct phyletic...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491342/ https://www.ncbi.nlm.nih.gov/pubmed/26026797 http://dx.doi.org/10.1007/s10482-015-0496-1 |
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author | Golinska, Patrycja Zucchi, Tiago Domingues Silva, Leonardo Dahm, Hanna Goodfellow, Michael |
author_facet | Golinska, Patrycja Zucchi, Tiago Domingues Silva, Leonardo Dahm, Hanna Goodfellow, Michael |
author_sort | Golinska, Patrycja |
collection | PubMed |
description | Seven acidophilic actinobacteria isolated from humus and mineral layers of a spruce forest soil were examined using a polyphasic approach. Chemotaxonomic properties of the isolates were found to be consistent with their classification in the genus Actinospica. The strains formed a distinct phyletic line in the Actinospica 16S rRNA gene tree being most closely related to Actinospica robiniae DSM 44927(T) (98.7–99.3 % similarity). DNA:DNA relatedness between isolate CSCA57(T) and the type strain of A. robiniae was found to be low at 40.8 (±6.6) %. The isolates were shown to have many phenotypic properties in common and were distinguished readily from the type strains of Actinospica acidiphila and A. robiniae using a range of phenotypic features. On the basis of these data the seven isolates were considered to represent a new species for which the name Actinospica durhamensis sp. nov. is proposed. The type strain of the species is CSCA 57(T) (=DSM 46820(T) = NCIMB 14953(T)). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10482-015-0496-1) contains supplementary material, which is available to authorized users. |
format | Online Article Text |
id | pubmed-4491342 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-44913422015-07-08 Actinospica durhamensis sp. nov., isolated from a spruce forest soil Golinska, Patrycja Zucchi, Tiago Domingues Silva, Leonardo Dahm, Hanna Goodfellow, Michael Antonie Van Leeuwenhoek Original Paper Seven acidophilic actinobacteria isolated from humus and mineral layers of a spruce forest soil were examined using a polyphasic approach. Chemotaxonomic properties of the isolates were found to be consistent with their classification in the genus Actinospica. The strains formed a distinct phyletic line in the Actinospica 16S rRNA gene tree being most closely related to Actinospica robiniae DSM 44927(T) (98.7–99.3 % similarity). DNA:DNA relatedness between isolate CSCA57(T) and the type strain of A. robiniae was found to be low at 40.8 (±6.6) %. The isolates were shown to have many phenotypic properties in common and were distinguished readily from the type strains of Actinospica acidiphila and A. robiniae using a range of phenotypic features. On the basis of these data the seven isolates were considered to represent a new species for which the name Actinospica durhamensis sp. nov. is proposed. The type strain of the species is CSCA 57(T) (=DSM 46820(T) = NCIMB 14953(T)). ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s10482-015-0496-1) contains supplementary material, which is available to authorized users. Springer International Publishing 2015-05-31 2015 /pmc/articles/PMC4491342/ /pubmed/26026797 http://dx.doi.org/10.1007/s10482-015-0496-1 Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. |
spellingShingle | Original Paper Golinska, Patrycja Zucchi, Tiago Domingues Silva, Leonardo Dahm, Hanna Goodfellow, Michael Actinospica durhamensis sp. nov., isolated from a spruce forest soil |
title | Actinospica durhamensis sp. nov., isolated from a spruce forest soil |
title_full | Actinospica durhamensis sp. nov., isolated from a spruce forest soil |
title_fullStr | Actinospica durhamensis sp. nov., isolated from a spruce forest soil |
title_full_unstemmed | Actinospica durhamensis sp. nov., isolated from a spruce forest soil |
title_short | Actinospica durhamensis sp. nov., isolated from a spruce forest soil |
title_sort | actinospica durhamensis sp. nov., isolated from a spruce forest soil |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491342/ https://www.ncbi.nlm.nih.gov/pubmed/26026797 http://dx.doi.org/10.1007/s10482-015-0496-1 |
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