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Development of an Ex Vivo, Beating Heart Model for CT Myocardial Perfusion

Objective. To test the feasibility of a CT-compatible, ex vivo, perfused porcine heart model for myocardial perfusion CT imaging. Methods. One porcine heart was perfused according to Langendorff. Dynamic perfusion scanning was performed with a second-generation dual source CT scanner. Circulatory pa...

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Autores principales: Pelgrim, Gert Jan, Das, Marco, Haberland, Ulrike, Slump, Cees, Handayani, Astri, van Tuijl, Sjoerd, Stijnen, Marco, Klotz, Ernst, Oudkerk, Matthijs, Wildberger, Joachim E., Vliegenthart, Rozemarijn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491382/
https://www.ncbi.nlm.nih.gov/pubmed/26185756
http://dx.doi.org/10.1155/2015/412716
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author Pelgrim, Gert Jan
Das, Marco
Haberland, Ulrike
Slump, Cees
Handayani, Astri
van Tuijl, Sjoerd
Stijnen, Marco
Klotz, Ernst
Oudkerk, Matthijs
Wildberger, Joachim E.
Vliegenthart, Rozemarijn
author_facet Pelgrim, Gert Jan
Das, Marco
Haberland, Ulrike
Slump, Cees
Handayani, Astri
van Tuijl, Sjoerd
Stijnen, Marco
Klotz, Ernst
Oudkerk, Matthijs
Wildberger, Joachim E.
Vliegenthart, Rozemarijn
author_sort Pelgrim, Gert Jan
collection PubMed
description Objective. To test the feasibility of a CT-compatible, ex vivo, perfused porcine heart model for myocardial perfusion CT imaging. Methods. One porcine heart was perfused according to Langendorff. Dynamic perfusion scanning was performed with a second-generation dual source CT scanner. Circulatory parameters like blood flow, aortic pressure, and heart rate were monitored throughout the experiment. Stenosis was induced in the circumflex artery, controlled by a fractional flow reserve (FFR) pressure wire. CT-derived myocardial perfusion parameters were analysed at FFR of 1 to 0.10/0.0. Results. CT images did not show major artefacts due to interference of the model setup. The pacemaker-induced heart rhythm was generally stable at 70 beats per minute. During most of the experiment, blood flow was 0.9–1.0 L/min, and arterial pressure varied between 80 and 95 mm/Hg. Blood flow decreased and arterial pressure increased by approximately 10% after inducing a stenosis with FFR ≤ 0.50. Dynamic perfusion scanning was possible across the range of stenosis grades. Perfusion parameters of circumflex-perfused myocardial segments were affected at increasing stenosis grades. Conclusion. An adapted Langendorff porcine heart model is feasible in a CT environment. This model provides control over physiological parameters and may allow in-depth validation of quantitative CT perfusion techniques.
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spelling pubmed-44913822015-07-16 Development of an Ex Vivo, Beating Heart Model for CT Myocardial Perfusion Pelgrim, Gert Jan Das, Marco Haberland, Ulrike Slump, Cees Handayani, Astri van Tuijl, Sjoerd Stijnen, Marco Klotz, Ernst Oudkerk, Matthijs Wildberger, Joachim E. Vliegenthart, Rozemarijn Biomed Res Int Research Article Objective. To test the feasibility of a CT-compatible, ex vivo, perfused porcine heart model for myocardial perfusion CT imaging. Methods. One porcine heart was perfused according to Langendorff. Dynamic perfusion scanning was performed with a second-generation dual source CT scanner. Circulatory parameters like blood flow, aortic pressure, and heart rate were monitored throughout the experiment. Stenosis was induced in the circumflex artery, controlled by a fractional flow reserve (FFR) pressure wire. CT-derived myocardial perfusion parameters were analysed at FFR of 1 to 0.10/0.0. Results. CT images did not show major artefacts due to interference of the model setup. The pacemaker-induced heart rhythm was generally stable at 70 beats per minute. During most of the experiment, blood flow was 0.9–1.0 L/min, and arterial pressure varied between 80 and 95 mm/Hg. Blood flow decreased and arterial pressure increased by approximately 10% after inducing a stenosis with FFR ≤ 0.50. Dynamic perfusion scanning was possible across the range of stenosis grades. Perfusion parameters of circumflex-perfused myocardial segments were affected at increasing stenosis grades. Conclusion. An adapted Langendorff porcine heart model is feasible in a CT environment. This model provides control over physiological parameters and may allow in-depth validation of quantitative CT perfusion techniques. Hindawi Publishing Corporation 2015 2015-06-21 /pmc/articles/PMC4491382/ /pubmed/26185756 http://dx.doi.org/10.1155/2015/412716 Text en Copyright © 2015 Gert Jan Pelgrim et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pelgrim, Gert Jan
Das, Marco
Haberland, Ulrike
Slump, Cees
Handayani, Astri
van Tuijl, Sjoerd
Stijnen, Marco
Klotz, Ernst
Oudkerk, Matthijs
Wildberger, Joachim E.
Vliegenthart, Rozemarijn
Development of an Ex Vivo, Beating Heart Model for CT Myocardial Perfusion
title Development of an Ex Vivo, Beating Heart Model for CT Myocardial Perfusion
title_full Development of an Ex Vivo, Beating Heart Model for CT Myocardial Perfusion
title_fullStr Development of an Ex Vivo, Beating Heart Model for CT Myocardial Perfusion
title_full_unstemmed Development of an Ex Vivo, Beating Heart Model for CT Myocardial Perfusion
title_short Development of an Ex Vivo, Beating Heart Model for CT Myocardial Perfusion
title_sort development of an ex vivo, beating heart model for ct myocardial perfusion
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491382/
https://www.ncbi.nlm.nih.gov/pubmed/26185756
http://dx.doi.org/10.1155/2015/412716
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