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Single-fluorophore membrane transport activity sensors with dual-emission read-out

We recently described a series of genetically encoded, single-fluorophore-based sensors, termed AmTrac and MepTrac, which monitor membrane transporter activity in vivo (De Michele et al., 2013). However, being intensiometric, AmTrac and Meptrac are limited in their use for quantitative studies. Here...

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Detalles Bibliográficos
Autores principales: Ast, Cindy, De Michele, Roberto, Kumke, Michael U, Frommer, Wolf B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491562/
https://www.ncbi.nlm.nih.gov/pubmed/26090909
http://dx.doi.org/10.7554/eLife.07113