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Studying antibiotic–membrane interactions via X-ray diffraction and fluorescence microscopy

Antibiotic drug resistance is a serious issue for the treatment of bacterial infection. Understanding the resistance to antibiotics is a key issue for developing new drugs. We used penicillin and sulbactam as model antibiotics to study their interaction with model membranes. Cholesterol was used to...

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Autores principales: Sun, Yi-Ting, Huang, Ping-Yuan, Lin, Cheng-Hao, Lee, Kuan-Rong, Lee, Ming-Tao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491592/
https://www.ncbi.nlm.nih.gov/pubmed/26155459
http://dx.doi.org/10.1016/j.fob.2015.06.006
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author Sun, Yi-Ting
Huang, Ping-Yuan
Lin, Cheng-Hao
Lee, Kuan-Rong
Lee, Ming-Tao
author_facet Sun, Yi-Ting
Huang, Ping-Yuan
Lin, Cheng-Hao
Lee, Kuan-Rong
Lee, Ming-Tao
author_sort Sun, Yi-Ting
collection PubMed
description Antibiotic drug resistance is a serious issue for the treatment of bacterial infection. Understanding the resistance to antibiotics is a key issue for developing new drugs. We used penicillin and sulbactam as model antibiotics to study their interaction with model membranes. Cholesterol was used to target the membrane for comparison with the well-known insertion model. Lamellar X-ray diffraction (LXD) was used to determine membrane thickness using successive drug-to-lipid molar ratios. The aspiration method for a single giant unilamellar vesicle (GUV) was used to monitor the kinetic binding process of antibiotic–membrane interactions in an aqueous solution. Both penicillin and sulbactam are found positioned outside the model membrane, while cholesterol inserts perpendicularly into the hydrophobic region of the membrane in aqueous solution. This result provides structural insights for understanding the antibiotic–membrane interaction and the mechanism of antibiotics.
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spelling pubmed-44915922015-07-07 Studying antibiotic–membrane interactions via X-ray diffraction and fluorescence microscopy Sun, Yi-Ting Huang, Ping-Yuan Lin, Cheng-Hao Lee, Kuan-Rong Lee, Ming-Tao FEBS Open Bio Research article Antibiotic drug resistance is a serious issue for the treatment of bacterial infection. Understanding the resistance to antibiotics is a key issue for developing new drugs. We used penicillin and sulbactam as model antibiotics to study their interaction with model membranes. Cholesterol was used to target the membrane for comparison with the well-known insertion model. Lamellar X-ray diffraction (LXD) was used to determine membrane thickness using successive drug-to-lipid molar ratios. The aspiration method for a single giant unilamellar vesicle (GUV) was used to monitor the kinetic binding process of antibiotic–membrane interactions in an aqueous solution. Both penicillin and sulbactam are found positioned outside the model membrane, while cholesterol inserts perpendicularly into the hydrophobic region of the membrane in aqueous solution. This result provides structural insights for understanding the antibiotic–membrane interaction and the mechanism of antibiotics. Elsevier 2015-06-15 /pmc/articles/PMC4491592/ /pubmed/26155459 http://dx.doi.org/10.1016/j.fob.2015.06.006 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research article
Sun, Yi-Ting
Huang, Ping-Yuan
Lin, Cheng-Hao
Lee, Kuan-Rong
Lee, Ming-Tao
Studying antibiotic–membrane interactions via X-ray diffraction and fluorescence microscopy
title Studying antibiotic–membrane interactions via X-ray diffraction and fluorescence microscopy
title_full Studying antibiotic–membrane interactions via X-ray diffraction and fluorescence microscopy
title_fullStr Studying antibiotic–membrane interactions via X-ray diffraction and fluorescence microscopy
title_full_unstemmed Studying antibiotic–membrane interactions via X-ray diffraction and fluorescence microscopy
title_short Studying antibiotic–membrane interactions via X-ray diffraction and fluorescence microscopy
title_sort studying antibiotic–membrane interactions via x-ray diffraction and fluorescence microscopy
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491592/
https://www.ncbi.nlm.nih.gov/pubmed/26155459
http://dx.doi.org/10.1016/j.fob.2015.06.006
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