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Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system

Various spheroid formation techniques have been widely developed for efficient and reliable 3-D cell culture research. Although those efforts improved many aspects of spheroid generation, the procedures became complex and also required unusual laboratory equipment. Many recent techniques still invol...

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Autores principales: Han, Chungmin, Takayama, Shuichi, Park, Jaesung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491721/
https://www.ncbi.nlm.nih.gov/pubmed/26144552
http://dx.doi.org/10.1038/srep11891
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author Han, Chungmin
Takayama, Shuichi
Park, Jaesung
author_facet Han, Chungmin
Takayama, Shuichi
Park, Jaesung
author_sort Han, Chungmin
collection PubMed
description Various spheroid formation techniques have been widely developed for efficient and reliable 3-D cell culture research. Although those efforts improved many aspects of spheroid generation, the procedures became complex and also required unusual laboratory equipment. Many recent techniques still involve laborious pipetting steps for spheroid manipulation such as collection, distribution and reseeding. In this report, we used a density-controlled polyethylene glycol and dextran aqueous two phase system to generate spheroids that are both consistent in size and precisely size-controllable. Moreover, by adding a few drops of fresh medium to the wells the contain spheroids, they can be simply settled and attached to the culture surface due to reduced densities of the phases. This unique attribute of the technique significantly reduces the numerous pipetting steps of spheroid manipulation to a single pipetting; therefore, the errors from those steps are eliminated and the reliability and efficiency of a research can be maximized.
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spelling pubmed-44917212015-07-08 Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system Han, Chungmin Takayama, Shuichi Park, Jaesung Sci Rep Article Various spheroid formation techniques have been widely developed for efficient and reliable 3-D cell culture research. Although those efforts improved many aspects of spheroid generation, the procedures became complex and also required unusual laboratory equipment. Many recent techniques still involve laborious pipetting steps for spheroid manipulation such as collection, distribution and reseeding. In this report, we used a density-controlled polyethylene glycol and dextran aqueous two phase system to generate spheroids that are both consistent in size and precisely size-controllable. Moreover, by adding a few drops of fresh medium to the wells the contain spheroids, they can be simply settled and attached to the culture surface due to reduced densities of the phases. This unique attribute of the technique significantly reduces the numerous pipetting steps of spheroid manipulation to a single pipetting; therefore, the errors from those steps are eliminated and the reliability and efficiency of a research can be maximized. Nature Publishing Group 2015-07-06 /pmc/articles/PMC4491721/ /pubmed/26144552 http://dx.doi.org/10.1038/srep11891 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Han, Chungmin
Takayama, Shuichi
Park, Jaesung
Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system
title Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system
title_full Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system
title_fullStr Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system
title_full_unstemmed Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system
title_short Formation and manipulation of cell spheroids using a density adjusted PEG/DEX aqueous two phase system
title_sort formation and manipulation of cell spheroids using a density adjusted peg/dex aqueous two phase system
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4491721/
https://www.ncbi.nlm.nih.gov/pubmed/26144552
http://dx.doi.org/10.1038/srep11891
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