CrEdit: CRISPR mediated multi-loci gene integration in Saccharomyces cerevisiae

BACKGROUND: One of the bottlenecks in production of biochemicals and pharmaceuticals in Saccharomyces cerevisiae is stable and homogeneous expression of pathway genes. Integration of genes into the genome of the production organism is often a preferred option when compared to expression from episoma...

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Detalles Bibliográficos
Autores principales: Ronda, Carlotta, Maury, Jérôme, Jakočiu̅nas, Tadas, Baallal Jacobsen, Simo Abdessamad, Germann, Susanne Manuela, Harrison, Scott James, Borodina, Irina, Keasling, Jay D, Jensen, Michael Krogh, Nielsen, Alex Toftgaard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4492099/
https://www.ncbi.nlm.nih.gov/pubmed/26148499
http://dx.doi.org/10.1186/s12934-015-0288-3

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