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Measurement of Total and Free Urinary Phenol and Paraben Concentrations over the Course of Pregnancy: Assessing Reliability and Contamination of Specimens in the Norwegian Mother and Child Cohort Study

BACKGROUND: Exposures to environmental phenols and parabens may be harmful, especially in utero. Prior studies have demonstrated high within-person variability of urinary concentrations across pregnancy. OBJECTIVES: We sought to measure phenol and paraben biomarker concentrations for the Norwegian M...

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Autores principales: Guidry, Virginia T., Longnecker, Matthew P., Aase, Heidi, Eggesbø, Merete, Zeiner, Pål, Reichborn-Kjennerud, Ted, Knudsen, Gun P., Bertelsen, Randi J., Ye, Xiaoyun, Calafat, Antonia M., Engel, Stephanie M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: NLM-Export 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4492266/
https://www.ncbi.nlm.nih.gov/pubmed/25782115
http://dx.doi.org/10.1289/ehp.1408325
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author Guidry, Virginia T.
Longnecker, Matthew P.
Aase, Heidi
Eggesbø, Merete
Zeiner, Pål
Reichborn-Kjennerud, Ted
Knudsen, Gun P.
Bertelsen, Randi J.
Ye, Xiaoyun
Calafat, Antonia M.
Engel, Stephanie M.
author_facet Guidry, Virginia T.
Longnecker, Matthew P.
Aase, Heidi
Eggesbø, Merete
Zeiner, Pål
Reichborn-Kjennerud, Ted
Knudsen, Gun P.
Bertelsen, Randi J.
Ye, Xiaoyun
Calafat, Antonia M.
Engel, Stephanie M.
author_sort Guidry, Virginia T.
collection PubMed
description BACKGROUND: Exposures to environmental phenols and parabens may be harmful, especially in utero. Prior studies have demonstrated high within-person variability of urinary concentrations across pregnancy. OBJECTIVES: We sought to measure phenol and paraben biomarker concentrations for the Norwegian Mother and Child Cohort (MoBa) study, assess within-person variability, and investigate any possible external phenol or paraben contamination of specimens. METHODS: We collected three spot urine samples at approximately 17, 23, and 29 weeks gestation in a hospital setting and added a preservative containing ethyl paraben. We measured urinary concentrations and within-person variability for phenols and parabens in a MoBa sample (n = 45), including a subgroup of 15 participants previously randomly selected for a bisphenol A (BPA) exposure study who had unusually high total BPA concentrations. Additionally, we compared reliability results for total, conjugated, and free concentrations of phenolic compounds. RESULTS: We detected total and free BPA, butyl paraben, propyl paraben, and methyl paraben in 100% of samples, total benzophenone-3 in 95% of samples, and infrequently detected free benzophenone-3 and total and free 2,4-dichlorophenol and 2,5-dichlorophenol. Intraclass correlation coefficients (ICCs) for total, conjugated, and free concentrations ranged from relatively low for BPA to moderate for propyl paraben. ICCs were generally similar overall and by subgroup. CONCLUSIONS: Using conjugated concentrations improved reliability estimates only for BPA. Measuring total and free concentrations, an approach that may be useful for future studies, allowed us to identify likely BPA and butyl paraben contamination of archived MoBa urine specimens. CITATION: Guidry VT, Longnecker MP, Aase H, Eggesbø M, Zeiner P, Reichborn-Kjennerud T, Knudsen GP, Bertelsen RJ, Ye X, Calafat AM, Engel SM. 2015. Measurement of total and free urinary phenol and paraben concentrations over the course of pregnancy: assessing reliability and contamination of specimens in the Norwegian Mother and Child Cohort Study. Environ Health Perspect 123:705–711; http://dx.doi.org/10.1289/ehp.1408325
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spelling pubmed-44922662015-07-09 Measurement of Total and Free Urinary Phenol and Paraben Concentrations over the Course of Pregnancy: Assessing Reliability and Contamination of Specimens in the Norwegian Mother and Child Cohort Study Guidry, Virginia T. Longnecker, Matthew P. Aase, Heidi Eggesbø, Merete Zeiner, Pål Reichborn-Kjennerud, Ted Knudsen, Gun P. Bertelsen, Randi J. Ye, Xiaoyun Calafat, Antonia M. Engel, Stephanie M. Environ Health Perspect Research BACKGROUND: Exposures to environmental phenols and parabens may be harmful, especially in utero. Prior studies have demonstrated high within-person variability of urinary concentrations across pregnancy. OBJECTIVES: We sought to measure phenol and paraben biomarker concentrations for the Norwegian Mother and Child Cohort (MoBa) study, assess within-person variability, and investigate any possible external phenol or paraben contamination of specimens. METHODS: We collected three spot urine samples at approximately 17, 23, and 29 weeks gestation in a hospital setting and added a preservative containing ethyl paraben. We measured urinary concentrations and within-person variability for phenols and parabens in a MoBa sample (n = 45), including a subgroup of 15 participants previously randomly selected for a bisphenol A (BPA) exposure study who had unusually high total BPA concentrations. Additionally, we compared reliability results for total, conjugated, and free concentrations of phenolic compounds. RESULTS: We detected total and free BPA, butyl paraben, propyl paraben, and methyl paraben in 100% of samples, total benzophenone-3 in 95% of samples, and infrequently detected free benzophenone-3 and total and free 2,4-dichlorophenol and 2,5-dichlorophenol. Intraclass correlation coefficients (ICCs) for total, conjugated, and free concentrations ranged from relatively low for BPA to moderate for propyl paraben. ICCs were generally similar overall and by subgroup. CONCLUSIONS: Using conjugated concentrations improved reliability estimates only for BPA. Measuring total and free concentrations, an approach that may be useful for future studies, allowed us to identify likely BPA and butyl paraben contamination of archived MoBa urine specimens. CITATION: Guidry VT, Longnecker MP, Aase H, Eggesbø M, Zeiner P, Reichborn-Kjennerud T, Knudsen GP, Bertelsen RJ, Ye X, Calafat AM, Engel SM. 2015. Measurement of total and free urinary phenol and paraben concentrations over the course of pregnancy: assessing reliability and contamination of specimens in the Norwegian Mother and Child Cohort Study. Environ Health Perspect 123:705–711; http://dx.doi.org/10.1289/ehp.1408325 NLM-Export 2015-03-17 2015-07 /pmc/articles/PMC4492266/ /pubmed/25782115 http://dx.doi.org/10.1289/ehp.1408325 Text en http://creativecommons.org/publicdomain/mark/1.0/ Publication of EHP lies in the public domain and is therefore without copyright. All text from EHP may be reprinted freely. Use of materials published in EHP should be acknowledged (for example, “Reproduced with permission from Environmental Health Perspectives”); pertinent reference information should be provided for the article from which the material was reproduced. Articles from EHP, especially the News section, may contain photographs or illustrations copyrighted by other commercial organizations or individuals that may not be used without obtaining prior approval from the holder of the copyright.
spellingShingle Research
Guidry, Virginia T.
Longnecker, Matthew P.
Aase, Heidi
Eggesbø, Merete
Zeiner, Pål
Reichborn-Kjennerud, Ted
Knudsen, Gun P.
Bertelsen, Randi J.
Ye, Xiaoyun
Calafat, Antonia M.
Engel, Stephanie M.
Measurement of Total and Free Urinary Phenol and Paraben Concentrations over the Course of Pregnancy: Assessing Reliability and Contamination of Specimens in the Norwegian Mother and Child Cohort Study
title Measurement of Total and Free Urinary Phenol and Paraben Concentrations over the Course of Pregnancy: Assessing Reliability and Contamination of Specimens in the Norwegian Mother and Child Cohort Study
title_full Measurement of Total and Free Urinary Phenol and Paraben Concentrations over the Course of Pregnancy: Assessing Reliability and Contamination of Specimens in the Norwegian Mother and Child Cohort Study
title_fullStr Measurement of Total and Free Urinary Phenol and Paraben Concentrations over the Course of Pregnancy: Assessing Reliability and Contamination of Specimens in the Norwegian Mother and Child Cohort Study
title_full_unstemmed Measurement of Total and Free Urinary Phenol and Paraben Concentrations over the Course of Pregnancy: Assessing Reliability and Contamination of Specimens in the Norwegian Mother and Child Cohort Study
title_short Measurement of Total and Free Urinary Phenol and Paraben Concentrations over the Course of Pregnancy: Assessing Reliability and Contamination of Specimens in the Norwegian Mother and Child Cohort Study
title_sort measurement of total and free urinary phenol and paraben concentrations over the course of pregnancy: assessing reliability and contamination of specimens in the norwegian mother and child cohort study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4492266/
https://www.ncbi.nlm.nih.gov/pubmed/25782115
http://dx.doi.org/10.1289/ehp.1408325
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