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Clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer

PURPOSE: Fascin-1 promotes the formation of filopodia, lamellipodia, and microspikes of cell membrane after its cross-linking with F-actin, thereby enhancing the cell movement and metastasis and invasion of tumor cells. This study explored the fascin-1 protein’s expression in non-small cell lung can...

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Autores principales: Ling, Xiao-Ling, Zhang, Tao, Hou, Xiao-Ming, Zhao, Da
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4492659/
https://www.ncbi.nlm.nih.gov/pubmed/26170694
http://dx.doi.org/10.2147/OTT.S84308
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author Ling, Xiao-Ling
Zhang, Tao
Hou, Xiao-Ming
Zhao, Da
author_facet Ling, Xiao-Ling
Zhang, Tao
Hou, Xiao-Ming
Zhao, Da
author_sort Ling, Xiao-Ling
collection PubMed
description PURPOSE: Fascin-1 promotes the formation of filopodia, lamellipodia, and microspikes of cell membrane after its cross-linking with F-actin, thereby enhancing the cell movement and metastasis and invasion of tumor cells. This study explored the fascin-1 protein’s expression in non-small cell lung cancer (NSCLC) tissues and its relationship with clinical pathology and prognostic indicators. METHODS: Immunohistochemical analysis was used to determine the expression of fascin-1 in NSCLC tissues. We used quantitative real-time polymerase chain reaction and western blot analysis to further verify the results. The fascin-1 expression and statistical method for clinical pathological parameters are examined by χ(2). Kaplan–Meier method is used for survival analysis. Cox’s Proportional Hazard Model was used to conduct a combined-effect analysis for each covariate. RESULTS: In 73 of the 128 cases, NSCLC cancer tissues (57.0%) were found with high expression of fascin-1, which was significantly higher than the adjacent tissues (35/128, 27.3%). The results suggested that the high expression of fascin-1 was significantly correlated with lymph node metastasis (P=0.022) and TNM stage (P=0.042). The high fascin-1 expression patients survived shorter than those NSCLC patients with low fascin-1 expression (P<0.05). Univariate analysis revealed that lymph node metastasis, TNM stage, and fascin-1 expression status were correlated with the overall survival. Similarly, lymph node metastasis, TNM stage, and fascin-1 expression status were significantly associated with the overall survival in multivariate analyses by using the Cox regression model. CONCLUSION: The fascin-1 protein may be a useful prognostic indicator and hopeful new target for NSCLC patients.
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spelling pubmed-44926592015-07-13 Clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer Ling, Xiao-Ling Zhang, Tao Hou, Xiao-Ming Zhao, Da Onco Targets Ther Original Research PURPOSE: Fascin-1 promotes the formation of filopodia, lamellipodia, and microspikes of cell membrane after its cross-linking with F-actin, thereby enhancing the cell movement and metastasis and invasion of tumor cells. This study explored the fascin-1 protein’s expression in non-small cell lung cancer (NSCLC) tissues and its relationship with clinical pathology and prognostic indicators. METHODS: Immunohistochemical analysis was used to determine the expression of fascin-1 in NSCLC tissues. We used quantitative real-time polymerase chain reaction and western blot analysis to further verify the results. The fascin-1 expression and statistical method for clinical pathological parameters are examined by χ(2). Kaplan–Meier method is used for survival analysis. Cox’s Proportional Hazard Model was used to conduct a combined-effect analysis for each covariate. RESULTS: In 73 of the 128 cases, NSCLC cancer tissues (57.0%) were found with high expression of fascin-1, which was significantly higher than the adjacent tissues (35/128, 27.3%). The results suggested that the high expression of fascin-1 was significantly correlated with lymph node metastasis (P=0.022) and TNM stage (P=0.042). The high fascin-1 expression patients survived shorter than those NSCLC patients with low fascin-1 expression (P<0.05). Univariate analysis revealed that lymph node metastasis, TNM stage, and fascin-1 expression status were correlated with the overall survival. Similarly, lymph node metastasis, TNM stage, and fascin-1 expression status were significantly associated with the overall survival in multivariate analyses by using the Cox regression model. CONCLUSION: The fascin-1 protein may be a useful prognostic indicator and hopeful new target for NSCLC patients. Dove Medical Press 2015-06-30 /pmc/articles/PMC4492659/ /pubmed/26170694 http://dx.doi.org/10.2147/OTT.S84308 Text en © 2015 Ling et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Ling, Xiao-Ling
Zhang, Tao
Hou, Xiao-Ming
Zhao, Da
Clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer
title Clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer
title_full Clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer
title_fullStr Clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer
title_full_unstemmed Clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer
title_short Clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer
title_sort clinicopathological significance of fascin-1 expression in patients with non-small cell lung cancer
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4492659/
https://www.ncbi.nlm.nih.gov/pubmed/26170694
http://dx.doi.org/10.2147/OTT.S84308
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