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The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection
The oral microbial community (microbiota) plays a critical role in human health and disease. Alterations in the oral microbiota may be associated with disorders such as gingivitis, periodontitis, childhood caries, alveolar osteitis, oral candidiasis and endodontic infections. In the immunosuppressed...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4492946/ https://www.ncbi.nlm.nih.gov/pubmed/26146997 http://dx.doi.org/10.1371/journal.pone.0131615 |
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author | Goldberg, Brittany E. Mongodin, Emmanuel F. Jones, Cheron E. Chung, Michelle Fraser, Claire M. Tate, Anupama Zeichner, Steven L. |
author_facet | Goldberg, Brittany E. Mongodin, Emmanuel F. Jones, Cheron E. Chung, Michelle Fraser, Claire M. Tate, Anupama Zeichner, Steven L. |
author_sort | Goldberg, Brittany E. |
collection | PubMed |
description | The oral microbial community (microbiota) plays a critical role in human health and disease. Alterations in the oral microbiota may be associated with disorders such as gingivitis, periodontitis, childhood caries, alveolar osteitis, oral candidiasis and endodontic infections. In the immunosuppressed population, the spectrum of potential oral disease is even broader, encompassing candidiasis, necrotizing gingivitis, parotid gland enlargement, Kaposi’s sarcoma, oral warts and other diseases. Here, we used 454 pyrosequencing of bacterial 16S rRNA genes to examine the oral microbiome of saliva, mucosal and tooth samples from HIV-positive and negative children. Patient demographics and clinical characteristics were collected from a cross-section of patients undergoing routine dental care. Multiple specimens from different sampling sites in the mouth were collected for each patient. The goal of the study was to observe the potential diversity of the oral microbiota among individual patients, sample locations, HIV status and various dental characteristics. We found that there were significant differences in the microbiome among the enrolled patients, and between sampling locations. The analysis was complicated by uneven enrollment in the patient cohorts, with only five HIV-negative patients enrolled in the study and by the rapid improvement in the health of HIV-infected children between the time the study was conceived and completed. The generally good oral health of the HIV-negative patients limited the number of dental plaque samples that could be collected. We did not identify significant differences between well-controlled HIV-positive patients and HIV-negative controls, suggesting that well-controlled HIV-positive patients essentially harbor similar oral flora compared to patients without HIV. Nor were significant differences in the oral microbiota identified between different teeth or with different dental characteristics. Additional studies are needed to better characterize the oral microbiome in children and those with poorly-controlled HIV infections. |
format | Online Article Text |
id | pubmed-4492946 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44929462015-07-15 The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection Goldberg, Brittany E. Mongodin, Emmanuel F. Jones, Cheron E. Chung, Michelle Fraser, Claire M. Tate, Anupama Zeichner, Steven L. PLoS One Research Article The oral microbial community (microbiota) plays a critical role in human health and disease. Alterations in the oral microbiota may be associated with disorders such as gingivitis, periodontitis, childhood caries, alveolar osteitis, oral candidiasis and endodontic infections. In the immunosuppressed population, the spectrum of potential oral disease is even broader, encompassing candidiasis, necrotizing gingivitis, parotid gland enlargement, Kaposi’s sarcoma, oral warts and other diseases. Here, we used 454 pyrosequencing of bacterial 16S rRNA genes to examine the oral microbiome of saliva, mucosal and tooth samples from HIV-positive and negative children. Patient demographics and clinical characteristics were collected from a cross-section of patients undergoing routine dental care. Multiple specimens from different sampling sites in the mouth were collected for each patient. The goal of the study was to observe the potential diversity of the oral microbiota among individual patients, sample locations, HIV status and various dental characteristics. We found that there were significant differences in the microbiome among the enrolled patients, and between sampling locations. The analysis was complicated by uneven enrollment in the patient cohorts, with only five HIV-negative patients enrolled in the study and by the rapid improvement in the health of HIV-infected children between the time the study was conceived and completed. The generally good oral health of the HIV-negative patients limited the number of dental plaque samples that could be collected. We did not identify significant differences between well-controlled HIV-positive patients and HIV-negative controls, suggesting that well-controlled HIV-positive patients essentially harbor similar oral flora compared to patients without HIV. Nor were significant differences in the oral microbiota identified between different teeth or with different dental characteristics. Additional studies are needed to better characterize the oral microbiome in children and those with poorly-controlled HIV infections. Public Library of Science 2015-07-06 /pmc/articles/PMC4492946/ /pubmed/26146997 http://dx.doi.org/10.1371/journal.pone.0131615 Text en https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article Goldberg, Brittany E. Mongodin, Emmanuel F. Jones, Cheron E. Chung, Michelle Fraser, Claire M. Tate, Anupama Zeichner, Steven L. The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection |
title | The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection |
title_full | The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection |
title_fullStr | The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection |
title_full_unstemmed | The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection |
title_short | The Oral Bacterial Communities of Children with Well-Controlled HIV Infection and without HIV Infection |
title_sort | oral bacterial communities of children with well-controlled hiv infection and without hiv infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4492946/ https://www.ncbi.nlm.nih.gov/pubmed/26146997 http://dx.doi.org/10.1371/journal.pone.0131615 |
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