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Engineering and Validation of a Vector for Concomitant Expression of Rare Transfer RNA (tRNA) and HIV-1 nef Genes in Escherichia coli

Relative ease in handling and manipulation of Escherichia coli strains make them primary candidate to express proteins heterologously. Overexpression of heterologous genes that contain codons infrequently used by E. coli is related with difficulties such as mRNA instability, early termination of tra...

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Detalles Bibliográficos
Autores principales:	Mualif, Siti Aisyah, Teow, Sin-Yeang, Omar, Tasyriq Che, Chew, Yik Wei, Yusoff, Narazah Mohd, Ali, Syed A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2015
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4492947/ https://www.ncbi.nlm.nih.gov/pubmed/26147991 http://dx.doi.org/10.1371/journal.pone.0130446

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