Characterization of the Prokaryotic Sodium Channel Na(v)Sp Pore with a Microfluidic Bilayer Platform

This paper describes the use of a newly-developed micro-chip bilayer platform to examine the electrophysiological properties of the prokaryotic voltage-gated sodium channel pore (Na(v)Sp) from Silicibacter pomeroyi. The platform allows up to 6 bilayers to be analysed simultaneously. Proteoliposomes...

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Detalles Bibliográficos
Autores principales: Saha, Shimul Chandra, Henderson, Alexander J., Powl, Andrew M., Wallace, B. A., de Planque, Maurits R. R., Morgan, Hywel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493117/
https://www.ncbi.nlm.nih.gov/pubmed/26147601
http://dx.doi.org/10.1371/journal.pone.0131286
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author Saha, Shimul Chandra
Henderson, Alexander J.
Powl, Andrew M.
Wallace, B. A.
de Planque, Maurits R. R.
Morgan, Hywel
author_facet Saha, Shimul Chandra
Henderson, Alexander J.
Powl, Andrew M.
Wallace, B. A.
de Planque, Maurits R. R.
Morgan, Hywel
author_sort Saha, Shimul Chandra
collection PubMed
description This paper describes the use of a newly-developed micro-chip bilayer platform to examine the electrophysiological properties of the prokaryotic voltage-gated sodium channel pore (Na(v)Sp) from Silicibacter pomeroyi. The platform allows up to 6 bilayers to be analysed simultaneously. Proteoliposomes were incorporated into suspended lipid bilayers formed within the microfluidic bilayer chips. The chips provide access to bilayers from either side, enabling the fast and controlled titration of compounds. Dose-dependent modulation of the opening probability by the channel blocking drug nifedipine was measured and its IC(50) determined.
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spelling pubmed-44931172015-07-15 Characterization of the Prokaryotic Sodium Channel Na(v)Sp Pore with a Microfluidic Bilayer Platform Saha, Shimul Chandra Henderson, Alexander J. Powl, Andrew M. Wallace, B. A. de Planque, Maurits R. R. Morgan, Hywel PLoS One Research Article This paper describes the use of a newly-developed micro-chip bilayer platform to examine the electrophysiological properties of the prokaryotic voltage-gated sodium channel pore (Na(v)Sp) from Silicibacter pomeroyi. The platform allows up to 6 bilayers to be analysed simultaneously. Proteoliposomes were incorporated into suspended lipid bilayers formed within the microfluidic bilayer chips. The chips provide access to bilayers from either side, enabling the fast and controlled titration of compounds. Dose-dependent modulation of the opening probability by the channel blocking drug nifedipine was measured and its IC(50) determined. Public Library of Science 2015-07-06 /pmc/articles/PMC4493117/ /pubmed/26147601 http://dx.doi.org/10.1371/journal.pone.0131286 Text en © 2015 Saha et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Saha, Shimul Chandra
Henderson, Alexander J.
Powl, Andrew M.
Wallace, B. A.
de Planque, Maurits R. R.
Morgan, Hywel
Characterization of the Prokaryotic Sodium Channel Na(v)Sp Pore with a Microfluidic Bilayer Platform
title Characterization of the Prokaryotic Sodium Channel Na(v)Sp Pore with a Microfluidic Bilayer Platform
title_full Characterization of the Prokaryotic Sodium Channel Na(v)Sp Pore with a Microfluidic Bilayer Platform
title_fullStr Characterization of the Prokaryotic Sodium Channel Na(v)Sp Pore with a Microfluidic Bilayer Platform
title_full_unstemmed Characterization of the Prokaryotic Sodium Channel Na(v)Sp Pore with a Microfluidic Bilayer Platform
title_short Characterization of the Prokaryotic Sodium Channel Na(v)Sp Pore with a Microfluidic Bilayer Platform
title_sort characterization of the prokaryotic sodium channel na(v)sp pore with a microfluidic bilayer platform
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493117/
https://www.ncbi.nlm.nih.gov/pubmed/26147601
http://dx.doi.org/10.1371/journal.pone.0131286
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