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Characterization of a Pre-Clinical Mini-Pig Model of Scaphoid Non-Union

A fractured scaphoid is a common disabling injury that is frequently complicated by non-union. The treatment of non-union remains challenging because of the scaphoid’s small size and delicate blood supply. Large animal models are the most reliable method to evaluate the efficacy of new treatment mod...

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Autores principales: Behrends, Dominique Andre, Khendek, Leticia, Gao, Chan, Zayed, Nadia, Henderson, Janet Elizabeth, Martineau, Paul Andre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493521/
https://www.ncbi.nlm.nih.gov/pubmed/26086923
http://dx.doi.org/10.3390/jfb6020407
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author Behrends, Dominique Andre
Khendek, Leticia
Gao, Chan
Zayed, Nadia
Henderson, Janet Elizabeth
Martineau, Paul Andre
author_facet Behrends, Dominique Andre
Khendek, Leticia
Gao, Chan
Zayed, Nadia
Henderson, Janet Elizabeth
Martineau, Paul Andre
author_sort Behrends, Dominique Andre
collection PubMed
description A fractured scaphoid is a common disabling injury that is frequently complicated by non-union. The treatment of non-union remains challenging because of the scaphoid’s small size and delicate blood supply. Large animal models are the most reliable method to evaluate the efficacy of new treatment modalities before their translation into clinical practice. The goal of this study was to model a human scaphoid fracture complicated by non-union in Yucatan mini-pigs. Imaging and perfusion studies were used to confirm that the anatomy and blood supply of the radiocarpal bone in mini-pigs were similar to the human scaphoid. A 3 mm osteotomy of the radiocarpal bone was generated and treated with immediate fixation or filled with a dense collagen gel followed by delayed fixation. Bone healing was assessed using quantitative micro computed tomography and histology. With immediate fixation, the osteotomy site was filled with new bone across its whole length resulting in complete bridging. The dense collagen gel, previously shown to impede neo-vascularization, followed by delayed fixation resulted in impaired bridging with less bone of lower quality. This model is an appropriate, easily reproducible model for the evaluation of novel approaches for the repair of human scaphoid fractures.
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spelling pubmed-44935212015-07-07 Characterization of a Pre-Clinical Mini-Pig Model of Scaphoid Non-Union Behrends, Dominique Andre Khendek, Leticia Gao, Chan Zayed, Nadia Henderson, Janet Elizabeth Martineau, Paul Andre J Funct Biomater Article A fractured scaphoid is a common disabling injury that is frequently complicated by non-union. The treatment of non-union remains challenging because of the scaphoid’s small size and delicate blood supply. Large animal models are the most reliable method to evaluate the efficacy of new treatment modalities before their translation into clinical practice. The goal of this study was to model a human scaphoid fracture complicated by non-union in Yucatan mini-pigs. Imaging and perfusion studies were used to confirm that the anatomy and blood supply of the radiocarpal bone in mini-pigs were similar to the human scaphoid. A 3 mm osteotomy of the radiocarpal bone was generated and treated with immediate fixation or filled with a dense collagen gel followed by delayed fixation. Bone healing was assessed using quantitative micro computed tomography and histology. With immediate fixation, the osteotomy site was filled with new bone across its whole length resulting in complete bridging. The dense collagen gel, previously shown to impede neo-vascularization, followed by delayed fixation resulted in impaired bridging with less bone of lower quality. This model is an appropriate, easily reproducible model for the evaluation of novel approaches for the repair of human scaphoid fractures. MDPI 2015-06-16 /pmc/articles/PMC4493521/ /pubmed/26086923 http://dx.doi.org/10.3390/jfb6020407 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Behrends, Dominique Andre
Khendek, Leticia
Gao, Chan
Zayed, Nadia
Henderson, Janet Elizabeth
Martineau, Paul Andre
Characterization of a Pre-Clinical Mini-Pig Model of Scaphoid Non-Union
title Characterization of a Pre-Clinical Mini-Pig Model of Scaphoid Non-Union
title_full Characterization of a Pre-Clinical Mini-Pig Model of Scaphoid Non-Union
title_fullStr Characterization of a Pre-Clinical Mini-Pig Model of Scaphoid Non-Union
title_full_unstemmed Characterization of a Pre-Clinical Mini-Pig Model of Scaphoid Non-Union
title_short Characterization of a Pre-Clinical Mini-Pig Model of Scaphoid Non-Union
title_sort characterization of a pre-clinical mini-pig model of scaphoid non-union
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493521/
https://www.ncbi.nlm.nih.gov/pubmed/26086923
http://dx.doi.org/10.3390/jfb6020407
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