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Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer

Breast cancer is the leading cause of cancer-related mortality for females worldwide [1]. Improving early screening strategies and understanding the events that lead to tumor initiation have led to demonstrable improvements in clinical outcome. Our previous work revealed a variance in the tumorigeni...

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Detalles Bibliográficos
Autores principales: Borcherding, Nicholas, Bormann, Nicholas, Kusner, David, Kolb, Ryan, Zhang, Weizhou
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493742/
https://www.ncbi.nlm.nih.gov/pubmed/26167451
http://dx.doi.org/10.1016/j.gdata.2015.04.008
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author Borcherding, Nicholas
Bormann, Nicholas
Kusner, David
Kolb, Ryan
Zhang, Weizhou
author_facet Borcherding, Nicholas
Bormann, Nicholas
Kusner, David
Kolb, Ryan
Zhang, Weizhou
author_sort Borcherding, Nicholas
collection PubMed
description Breast cancer is the leading cause of cancer-related mortality for females worldwide [1]. Improving early screening strategies and understanding the events that lead to tumor initiation have led to demonstrable improvements in clinical outcome. Our previous work revealed a variance in the tumorigenic capacity between different mammary epithelial cell populations in an MMTV-ErbB2 mouse model. In order to greater understand how different mammary epithelial cells influence the tumorigenic capacity in ErbB2-induced breast cancer, we transplanted different cell populations from pre-neoplastic MMTV-ErbB2 female mice into recipient mice for tumorigenic study. We found that different mammary epithelial cells bear different tumorigenic potentials even when induced by the same ErbB2 proto-oncogene. To understand the difference in tumors formed from different epithelial cells, we performed gene expression profiling using these tumors (GSE64487). Several genes were further validated using real-time reverse transcription polymerase chain reaction (RT-PCR). Here we provide further details on the experimental methods and microarray analysis. This data provides a resource to further understanding how different mammary cell populations can initiate ErbB2-driven tumors and the role of these cell populations as putative tumor-initiating cells (TICs).
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spelling pubmed-44937422015-10-19 Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer Borcherding, Nicholas Bormann, Nicholas Kusner, David Kolb, Ryan Zhang, Weizhou Genom Data Data in Brief Breast cancer is the leading cause of cancer-related mortality for females worldwide [1]. Improving early screening strategies and understanding the events that lead to tumor initiation have led to demonstrable improvements in clinical outcome. Our previous work revealed a variance in the tumorigenic capacity between different mammary epithelial cell populations in an MMTV-ErbB2 mouse model. In order to greater understand how different mammary epithelial cells influence the tumorigenic capacity in ErbB2-induced breast cancer, we transplanted different cell populations from pre-neoplastic MMTV-ErbB2 female mice into recipient mice for tumorigenic study. We found that different mammary epithelial cells bear different tumorigenic potentials even when induced by the same ErbB2 proto-oncogene. To understand the difference in tumors formed from different epithelial cells, we performed gene expression profiling using these tumors (GSE64487). Several genes were further validated using real-time reverse transcription polymerase chain reaction (RT-PCR). Here we provide further details on the experimental methods and microarray analysis. This data provides a resource to further understanding how different mammary cell populations can initiate ErbB2-driven tumors and the role of these cell populations as putative tumor-initiating cells (TICs). Elsevier 2015-04-17 /pmc/articles/PMC4493742/ /pubmed/26167451 http://dx.doi.org/10.1016/j.gdata.2015.04.008 Text en © 2015 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Data in Brief
Borcherding, Nicholas
Bormann, Nicholas
Kusner, David
Kolb, Ryan
Zhang, Weizhou
Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer
title Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer
title_full Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer
title_fullStr Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer
title_full_unstemmed Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer
title_short Transcriptome analysis of basal and luminal tumor-initiating cells in ErbB2-driven breast cancer
title_sort transcriptome analysis of basal and luminal tumor-initiating cells in erbb2-driven breast cancer
topic Data in Brief
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493742/
https://www.ncbi.nlm.nih.gov/pubmed/26167451
http://dx.doi.org/10.1016/j.gdata.2015.04.008
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