The ciliary transition zone functions in cell adhesion but is dispensable for axoneme assembly in C. elegans

Cilia are cellular projections that perform sensory and motile functions. A key ciliary subdomain is the transition zone, which lies between basal body and axoneme. Previous work in Caenorhabditis elegans identified two ciliopathy-associated protein complexes or modules that direct assembly of trans...

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Autores principales: Schouteden, Clementine, Serwas, Daniel, Palfy, Mate, Dammermann, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493997/
https://www.ncbi.nlm.nih.gov/pubmed/26124290
http://dx.doi.org/10.1083/jcb.201501013
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author Schouteden, Clementine
Serwas, Daniel
Palfy, Mate
Dammermann, Alexander
author_facet Schouteden, Clementine
Serwas, Daniel
Palfy, Mate
Dammermann, Alexander
author_sort Schouteden, Clementine
collection PubMed
description Cilia are cellular projections that perform sensory and motile functions. A key ciliary subdomain is the transition zone, which lies between basal body and axoneme. Previous work in Caenorhabditis elegans identified two ciliopathy-associated protein complexes or modules that direct assembly of transition zone Y-links. Here, we identify C. elegans CEP290 as a component of a third module required to form an inner scaffolding structure called the central cylinder. Co-inhibition of all three modules completely disrupted transition zone structure. Surprisingly, axoneme assembly was only mildly perturbed. However, dendrite extension by retrograde migration was strongly impaired, revealing an unexpected role for the transition zone in cell adhesion.
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spelling pubmed-44939972016-01-06 The ciliary transition zone functions in cell adhesion but is dispensable for axoneme assembly in C. elegans Schouteden, Clementine Serwas, Daniel Palfy, Mate Dammermann, Alexander J Cell Biol Research Articles Cilia are cellular projections that perform sensory and motile functions. A key ciliary subdomain is the transition zone, which lies between basal body and axoneme. Previous work in Caenorhabditis elegans identified two ciliopathy-associated protein complexes or modules that direct assembly of transition zone Y-links. Here, we identify C. elegans CEP290 as a component of a third module required to form an inner scaffolding structure called the central cylinder. Co-inhibition of all three modules completely disrupted transition zone structure. Surprisingly, axoneme assembly was only mildly perturbed. However, dendrite extension by retrograde migration was strongly impaired, revealing an unexpected role for the transition zone in cell adhesion. The Rockefeller University Press 2015-07-06 /pmc/articles/PMC4493997/ /pubmed/26124290 http://dx.doi.org/10.1083/jcb.201501013 Text en © 2015 Schouteden et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Schouteden, Clementine
Serwas, Daniel
Palfy, Mate
Dammermann, Alexander
The ciliary transition zone functions in cell adhesion but is dispensable for axoneme assembly in C. elegans
title The ciliary transition zone functions in cell adhesion but is dispensable for axoneme assembly in C. elegans
title_full The ciliary transition zone functions in cell adhesion but is dispensable for axoneme assembly in C. elegans
title_fullStr The ciliary transition zone functions in cell adhesion but is dispensable for axoneme assembly in C. elegans
title_full_unstemmed The ciliary transition zone functions in cell adhesion but is dispensable for axoneme assembly in C. elegans
title_short The ciliary transition zone functions in cell adhesion but is dispensable for axoneme assembly in C. elegans
title_sort ciliary transition zone functions in cell adhesion but is dispensable for axoneme assembly in c. elegans
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4493997/
https://www.ncbi.nlm.nih.gov/pubmed/26124290
http://dx.doi.org/10.1083/jcb.201501013
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