Cargando…
Vector Design for Improved DNA Vaccine Efficacy, Safety and Production
DNA vaccination is a disruptive technology that offers the promise of a new rapidly deployed vaccination platform to treat human and animal disease with gene-based materials. Innovations such as electroporation, needle free jet delivery and lipid-based carriers increase transgene expression and immu...
Autor principal: | |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2013
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494225/ https://www.ncbi.nlm.nih.gov/pubmed/26344110 http://dx.doi.org/10.3390/vaccines1030225 |
_version_ | 1782380048040329216 |
---|---|
author | Williams, James A. |
author_facet | Williams, James A. |
author_sort | Williams, James A. |
collection | PubMed |
description | DNA vaccination is a disruptive technology that offers the promise of a new rapidly deployed vaccination platform to treat human and animal disease with gene-based materials. Innovations such as electroporation, needle free jet delivery and lipid-based carriers increase transgene expression and immunogenicity through more effective gene delivery. This review summarizes complementary vector design innovations that, when combined with leading delivery platforms, further enhance DNA vaccine performance. These next generation vectors also address potential safety issues such as antibiotic selection, and increase plasmid manufacturing quality and yield in exemplary fermentation production processes. Application of optimized constructs in combination with improved delivery platforms tangibly improves the prospect of successful application of DNA vaccination as prophylactic vaccines for diverse human infectious disease targets or as therapeutic vaccines for cancer and allergy. |
format | Online Article Text |
id | pubmed-4494225 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2013 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-44942252015-08-31 Vector Design for Improved DNA Vaccine Efficacy, Safety and Production Williams, James A. Vaccines (Basel) Review DNA vaccination is a disruptive technology that offers the promise of a new rapidly deployed vaccination platform to treat human and animal disease with gene-based materials. Innovations such as electroporation, needle free jet delivery and lipid-based carriers increase transgene expression and immunogenicity through more effective gene delivery. This review summarizes complementary vector design innovations that, when combined with leading delivery platforms, further enhance DNA vaccine performance. These next generation vectors also address potential safety issues such as antibiotic selection, and increase plasmid manufacturing quality and yield in exemplary fermentation production processes. Application of optimized constructs in combination with improved delivery platforms tangibly improves the prospect of successful application of DNA vaccination as prophylactic vaccines for diverse human infectious disease targets or as therapeutic vaccines for cancer and allergy. MDPI 2013-06-25 /pmc/articles/PMC4494225/ /pubmed/26344110 http://dx.doi.org/10.3390/vaccines1030225 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
spellingShingle | Review Williams, James A. Vector Design for Improved DNA Vaccine Efficacy, Safety and Production |
title | Vector Design for Improved DNA Vaccine Efficacy, Safety and Production |
title_full | Vector Design for Improved DNA Vaccine Efficacy, Safety and Production |
title_fullStr | Vector Design for Improved DNA Vaccine Efficacy, Safety and Production |
title_full_unstemmed | Vector Design for Improved DNA Vaccine Efficacy, Safety and Production |
title_short | Vector Design for Improved DNA Vaccine Efficacy, Safety and Production |
title_sort | vector design for improved dna vaccine efficacy, safety and production |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494225/ https://www.ncbi.nlm.nih.gov/pubmed/26344110 http://dx.doi.org/10.3390/vaccines1030225 |
work_keys_str_mv | AT williamsjamesa vectordesignforimproveddnavaccineefficacysafetyandproduction |