Cargando…

Vector Design for Improved DNA Vaccine Efficacy, Safety and Production

DNA vaccination is a disruptive technology that offers the promise of a new rapidly deployed vaccination platform to treat human and animal disease with gene-based materials. Innovations such as electroporation, needle free jet delivery and lipid-based carriers increase transgene expression and immu...

Descripción completa

Detalles Bibliográficos
Autor principal: Williams, James A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494225/
https://www.ncbi.nlm.nih.gov/pubmed/26344110
http://dx.doi.org/10.3390/vaccines1030225
_version_ 1782380048040329216
author Williams, James A.
author_facet Williams, James A.
author_sort Williams, James A.
collection PubMed
description DNA vaccination is a disruptive technology that offers the promise of a new rapidly deployed vaccination platform to treat human and animal disease with gene-based materials. Innovations such as electroporation, needle free jet delivery and lipid-based carriers increase transgene expression and immunogenicity through more effective gene delivery. This review summarizes complementary vector design innovations that, when combined with leading delivery platforms, further enhance DNA vaccine performance. These next generation vectors also address potential safety issues such as antibiotic selection, and increase plasmid manufacturing quality and yield in exemplary fermentation production processes. Application of optimized constructs in combination with improved delivery platforms tangibly improves the prospect of successful application of DNA vaccination as prophylactic vaccines for diverse human infectious disease targets or as therapeutic vaccines for cancer and allergy.
format Online
Article
Text
id pubmed-4494225
institution National Center for Biotechnology Information
language English
publishDate 2013
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-44942252015-08-31 Vector Design for Improved DNA Vaccine Efficacy, Safety and Production Williams, James A. Vaccines (Basel) Review DNA vaccination is a disruptive technology that offers the promise of a new rapidly deployed vaccination platform to treat human and animal disease with gene-based materials. Innovations such as electroporation, needle free jet delivery and lipid-based carriers increase transgene expression and immunogenicity through more effective gene delivery. This review summarizes complementary vector design innovations that, when combined with leading delivery platforms, further enhance DNA vaccine performance. These next generation vectors also address potential safety issues such as antibiotic selection, and increase plasmid manufacturing quality and yield in exemplary fermentation production processes. Application of optimized constructs in combination with improved delivery platforms tangibly improves the prospect of successful application of DNA vaccination as prophylactic vaccines for diverse human infectious disease targets or as therapeutic vaccines for cancer and allergy. MDPI 2013-06-25 /pmc/articles/PMC4494225/ /pubmed/26344110 http://dx.doi.org/10.3390/vaccines1030225 Text en © 2013 by the authors; licensee MDPI, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0/ This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Review
Williams, James A.
Vector Design for Improved DNA Vaccine Efficacy, Safety and Production
title Vector Design for Improved DNA Vaccine Efficacy, Safety and Production
title_full Vector Design for Improved DNA Vaccine Efficacy, Safety and Production
title_fullStr Vector Design for Improved DNA Vaccine Efficacy, Safety and Production
title_full_unstemmed Vector Design for Improved DNA Vaccine Efficacy, Safety and Production
title_short Vector Design for Improved DNA Vaccine Efficacy, Safety and Production
title_sort vector design for improved dna vaccine efficacy, safety and production
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494225/
https://www.ncbi.nlm.nih.gov/pubmed/26344110
http://dx.doi.org/10.3390/vaccines1030225
work_keys_str_mv AT williamsjamesa vectordesignforimproveddnavaccineefficacysafetyandproduction