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Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future

Serological techniques commonly used to quantify influenza-specific antibodies include the Haemagglutination Inhibition (HI), Single Radial Haemolysis (SRH) and Virus Neutralization (VN) assays. HI and SRH are established and reproducible techniques, whereas VN is more demanding. Every new influenza...

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Autores principales: Trombetta, Claudia Maria, Perini, Daniele, Mather, Stuart, Temperton, Nigel, Montomoli, Emanuele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2014
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494249/
https://www.ncbi.nlm.nih.gov/pubmed/26344888
http://dx.doi.org/10.3390/vaccines2040707
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author Trombetta, Claudia Maria
Perini, Daniele
Mather, Stuart
Temperton, Nigel
Montomoli, Emanuele
author_facet Trombetta, Claudia Maria
Perini, Daniele
Mather, Stuart
Temperton, Nigel
Montomoli, Emanuele
author_sort Trombetta, Claudia Maria
collection PubMed
description Serological techniques commonly used to quantify influenza-specific antibodies include the Haemagglutination Inhibition (HI), Single Radial Haemolysis (SRH) and Virus Neutralization (VN) assays. HI and SRH are established and reproducible techniques, whereas VN is more demanding. Every new influenza vaccine needs to fulfil the strict criteria issued by the European Medicines Agency (EMA) in order to be licensed. These criteria currently apply exclusively to SRH and HI assays and refer to two different target groups—healthy adults and the elderly, but other vaccine recipient age groups have not been considered (i.e., children). The purpose of this timely review is to highlight the current scenario on correlates of protection concerning influenza vaccines and underline the need to revise the criteria and assays currently in use. In addition to SRH and HI assays, the technical advantages provided by other techniques such as the VN assay, pseudotype-based neutralization assay, neuraminidase and cell-mediated immunity assays need to be considered and regulated via EMA criteria, considering the many significant advantages that they could offer for the development of effective vaccines.
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spelling pubmed-44942492015-08-31 Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future Trombetta, Claudia Maria Perini, Daniele Mather, Stuart Temperton, Nigel Montomoli, Emanuele Vaccines (Basel) Review Serological techniques commonly used to quantify influenza-specific antibodies include the Haemagglutination Inhibition (HI), Single Radial Haemolysis (SRH) and Virus Neutralization (VN) assays. HI and SRH are established and reproducible techniques, whereas VN is more demanding. Every new influenza vaccine needs to fulfil the strict criteria issued by the European Medicines Agency (EMA) in order to be licensed. These criteria currently apply exclusively to SRH and HI assays and refer to two different target groups—healthy adults and the elderly, but other vaccine recipient age groups have not been considered (i.e., children). The purpose of this timely review is to highlight the current scenario on correlates of protection concerning influenza vaccines and underline the need to revise the criteria and assays currently in use. In addition to SRH and HI assays, the technical advantages provided by other techniques such as the VN assay, pseudotype-based neutralization assay, neuraminidase and cell-mediated immunity assays need to be considered and regulated via EMA criteria, considering the many significant advantages that they could offer for the development of effective vaccines. MDPI 2014-10-13 /pmc/articles/PMC4494249/ /pubmed/26344888 http://dx.doi.org/10.3390/vaccines2040707 Text en © 2014 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Trombetta, Claudia Maria
Perini, Daniele
Mather, Stuart
Temperton, Nigel
Montomoli, Emanuele
Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future
title Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future
title_full Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future
title_fullStr Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future
title_full_unstemmed Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future
title_short Overview of Serological Techniques for Influenza Vaccine Evaluation: Past, Present and Future
title_sort overview of serological techniques for influenza vaccine evaluation: past, present and future
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494249/
https://www.ncbi.nlm.nih.gov/pubmed/26344888
http://dx.doi.org/10.3390/vaccines2040707
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