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Cellular response of chondrocytes to magnesium alloys for orthopedic applications
In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO(4)·(2)H(2)O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface o...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494570/ https://www.ncbi.nlm.nih.gov/pubmed/25975216 http://dx.doi.org/10.3892/ijmm.2015.2211 |
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author | LIAO, YI XU, QINGLI ZHANG, JIAN NIU, JIALING YUAN, GUANGYIN JIANG, YAO HE, YAOHUA WANG, XINLING |
author_facet | LIAO, YI XU, QINGLI ZHANG, JIAN NIU, JIALING YUAN, GUANGYIN JIANG, YAO HE, YAOHUA WANG, XINLING |
author_sort | LIAO, YI |
collection | PubMed |
description | In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO(4)·(2)H(2)O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface of all samples. An indirect cytotoxicity test using MTT assay revealed that C-JDBM and TC4 exhibited results similar to those of the negative control, better than those obtained with JDBM, AZ31, WE43 and pure Mg (p<0.05). There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05). The results of indirect cell cytotoxicity and proliferation assays, as well as those of apoptosis assay, glycosaminoglycan (GAG) quantification, assessment of collagen II (Col II) levels and RT-qPCR revealed a similar a trend as was observed with MTT assay. These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg. Furthermore, CaHPO(4)·(2)H(2)O coating significantly improved the biocompatibility of this alloy. |
format | Online Article Text |
id | pubmed-4494570 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-44945702015-07-13 Cellular response of chondrocytes to magnesium alloys for orthopedic applications LIAO, YI XU, QINGLI ZHANG, JIAN NIU, JIALING YUAN, GUANGYIN JIANG, YAO HE, YAOHUA WANG, XINLING Int J Mol Med Articles In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO(4)·(2)H(2)O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface of all samples. An indirect cytotoxicity test using MTT assay revealed that C-JDBM and TC4 exhibited results similar to those of the negative control, better than those obtained with JDBM, AZ31, WE43 and pure Mg (p<0.05). There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05). The results of indirect cell cytotoxicity and proliferation assays, as well as those of apoptosis assay, glycosaminoglycan (GAG) quantification, assessment of collagen II (Col II) levels and RT-qPCR revealed a similar a trend as was observed with MTT assay. These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg. Furthermore, CaHPO(4)·(2)H(2)O coating significantly improved the biocompatibility of this alloy. D.A. Spandidos 2015-07 2015-05-14 /pmc/articles/PMC4494570/ /pubmed/25975216 http://dx.doi.org/10.3892/ijmm.2015.2211 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited. |
spellingShingle | Articles LIAO, YI XU, QINGLI ZHANG, JIAN NIU, JIALING YUAN, GUANGYIN JIANG, YAO HE, YAOHUA WANG, XINLING Cellular response of chondrocytes to magnesium alloys for orthopedic applications |
title | Cellular response of chondrocytes to magnesium alloys for orthopedic applications |
title_full | Cellular response of chondrocytes to magnesium alloys for orthopedic applications |
title_fullStr | Cellular response of chondrocytes to magnesium alloys for orthopedic applications |
title_full_unstemmed | Cellular response of chondrocytes to magnesium alloys for orthopedic applications |
title_short | Cellular response of chondrocytes to magnesium alloys for orthopedic applications |
title_sort | cellular response of chondrocytes to magnesium alloys for orthopedic applications |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494570/ https://www.ncbi.nlm.nih.gov/pubmed/25975216 http://dx.doi.org/10.3892/ijmm.2015.2211 |
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