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Cellular response of chondrocytes to magnesium alloys for orthopedic applications

In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO(4)·(2)H(2)O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface o...

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Autores principales: LIAO, YI, XU, QINGLI, ZHANG, JIAN, NIU, JIALING, YUAN, GUANGYIN, JIANG, YAO, HE, YAOHUA, WANG, XINLING
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494570/
https://www.ncbi.nlm.nih.gov/pubmed/25975216
http://dx.doi.org/10.3892/ijmm.2015.2211
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author LIAO, YI
XU, QINGLI
ZHANG, JIAN
NIU, JIALING
YUAN, GUANGYIN
JIANG, YAO
HE, YAOHUA
WANG, XINLING
author_facet LIAO, YI
XU, QINGLI
ZHANG, JIAN
NIU, JIALING
YUAN, GUANGYIN
JIANG, YAO
HE, YAOHUA
WANG, XINLING
author_sort LIAO, YI
collection PubMed
description In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO(4)·(2)H(2)O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface of all samples. An indirect cytotoxicity test using MTT assay revealed that C-JDBM and TC4 exhibited results similar to those of the negative control, better than those obtained with JDBM, AZ31, WE43 and pure Mg (p<0.05). There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05). The results of indirect cell cytotoxicity and proliferation assays, as well as those of apoptosis assay, glycosaminoglycan (GAG) quantification, assessment of collagen II (Col II) levels and RT-qPCR revealed a similar a trend as was observed with MTT assay. These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg. Furthermore, CaHPO(4)·(2)H(2)O coating significantly improved the biocompatibility of this alloy.
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spelling pubmed-44945702015-07-13 Cellular response of chondrocytes to magnesium alloys for orthopedic applications LIAO, YI XU, QINGLI ZHANG, JIAN NIU, JIALING YUAN, GUANGYIN JIANG, YAO HE, YAOHUA WANG, XINLING Int J Mol Med Articles In the present study, the effects of Mg-Nd-Zn-Zr (JDBM), brushite (CaHPO(4)·(2)H(2)O)-coated JDBM (C-JDBM), AZ31, WE43, pure magnesium (Mg) and Ti alloy (TC4) on rabbit chondrocytes were investigated in vitro. Adhesion experiments revealed the satisfactory morphology of chondrocytes on the surface of all samples. An indirect cytotoxicity test using MTT assay revealed that C-JDBM and TC4 exhibited results similar to those of the negative control, better than those obtained with JDBM, AZ31, WE43 and pure Mg (p<0.05). There were no statistically significant differences observed between the JDBM, AZ31, WE43 and pure Mg group (p>0.05). The results of indirect cell cytotoxicity and proliferation assays, as well as those of apoptosis assay, glycosaminoglycan (GAG) quantification, assessment of collagen II (Col II) levels and RT-qPCR revealed a similar a trend as was observed with MTT assay. These findings suggested that the JDBM alloy was highly biocompatible with chondrocytes in vitro, yielding results similar to those of AZ31, WE43 and pure Mg. Furthermore, CaHPO(4)·(2)H(2)O coating significantly improved the biocompatibility of this alloy. D.A. Spandidos 2015-07 2015-05-14 /pmc/articles/PMC4494570/ /pubmed/25975216 http://dx.doi.org/10.3892/ijmm.2015.2211 Text en Copyright © 2015, Spandidos Publications http://creativecommons.org/licenses/by/3.0 This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.
spellingShingle Articles
LIAO, YI
XU, QINGLI
ZHANG, JIAN
NIU, JIALING
YUAN, GUANGYIN
JIANG, YAO
HE, YAOHUA
WANG, XINLING
Cellular response of chondrocytes to magnesium alloys for orthopedic applications
title Cellular response of chondrocytes to magnesium alloys for orthopedic applications
title_full Cellular response of chondrocytes to magnesium alloys for orthopedic applications
title_fullStr Cellular response of chondrocytes to magnesium alloys for orthopedic applications
title_full_unstemmed Cellular response of chondrocytes to magnesium alloys for orthopedic applications
title_short Cellular response of chondrocytes to magnesium alloys for orthopedic applications
title_sort cellular response of chondrocytes to magnesium alloys for orthopedic applications
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4494570/
https://www.ncbi.nlm.nih.gov/pubmed/25975216
http://dx.doi.org/10.3892/ijmm.2015.2211
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