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Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere
BACKGROUND AND AIMS: To form nitrogen-fixing nodules on pea roots, Rhizobium leguminosarum biovar viciae must be competitive in the rhizosphere. Our aim was to identify genes important for rhizosphere fitness. METHODS: Signature-tagged mutants were screened using microarrays to identify mutants redu...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer International Publishing
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4495286/ https://www.ncbi.nlm.nih.gov/pubmed/26166901 http://dx.doi.org/10.1007/s11104-015-2389-5 |
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author | Garcia-Fraile, Paula Seaman, Jonathan C. Karunakaran, Ramakrishnan Edwards, Anne Poole, Philip S. Downie, J. Allan |
author_facet | Garcia-Fraile, Paula Seaman, Jonathan C. Karunakaran, Ramakrishnan Edwards, Anne Poole, Philip S. Downie, J. Allan |
author_sort | Garcia-Fraile, Paula |
collection | PubMed |
description | BACKGROUND AND AIMS: To form nitrogen-fixing nodules on pea roots, Rhizobium leguminosarum biovar viciae must be competitive in the rhizosphere. Our aim was to identify genes important for rhizosphere fitness. METHODS: Signature-tagged mutants were screened using microarrays to identify mutants reduced for growth in pea rhizospheres. Candidate mutants were assessed relative to controls for growth in minimal medium, growth in pea rhizospheres and for infection of peas in mixed inoculants. Mutated genes were identified by DNA sequencing and confirmed by transduction. RESULTS: Of 5508 signature-tagged mutants, microarrays implicated 50 as having decreased rhizosphere fitness. Growth tests identified six mutants with rhizosphere-specific phenotypes. The mutation in one of the genes (araE) was in an arabinose catabolism operon and blocked growth on arabinose. The mutation in another gene (pcaM), encoding a predicted solute binding protein for protocatechuate and hydroxybenzoate uptake, decreased growth on protocatechuate. Both mutants were decreased for nodule infection competitiveness with mixed inoculants, but nodulated peas normally when inoculated alone. Other mutants with similar phenotypes had mutations predicted to affect secondary metabolism. CONCLUSIONS: Catabolism of arabinose and protocatechuate in the pea rhizosphere is important for competitiveness of R.l. viciae. Other genes predicted to be involved in secondary metabolism are also important. |
format | Online Article Text |
id | pubmed-4495286 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Springer International Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-44952862015-07-09 Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere Garcia-Fraile, Paula Seaman, Jonathan C. Karunakaran, Ramakrishnan Edwards, Anne Poole, Philip S. Downie, J. Allan Plant Soil Regular Article BACKGROUND AND AIMS: To form nitrogen-fixing nodules on pea roots, Rhizobium leguminosarum biovar viciae must be competitive in the rhizosphere. Our aim was to identify genes important for rhizosphere fitness. METHODS: Signature-tagged mutants were screened using microarrays to identify mutants reduced for growth in pea rhizospheres. Candidate mutants were assessed relative to controls for growth in minimal medium, growth in pea rhizospheres and for infection of peas in mixed inoculants. Mutated genes were identified by DNA sequencing and confirmed by transduction. RESULTS: Of 5508 signature-tagged mutants, microarrays implicated 50 as having decreased rhizosphere fitness. Growth tests identified six mutants with rhizosphere-specific phenotypes. The mutation in one of the genes (araE) was in an arabinose catabolism operon and blocked growth on arabinose. The mutation in another gene (pcaM), encoding a predicted solute binding protein for protocatechuate and hydroxybenzoate uptake, decreased growth on protocatechuate. Both mutants were decreased for nodule infection competitiveness with mixed inoculants, but nodulated peas normally when inoculated alone. Other mutants with similar phenotypes had mutations predicted to affect secondary metabolism. CONCLUSIONS: Catabolism of arabinose and protocatechuate in the pea rhizosphere is important for competitiveness of R.l. viciae. Other genes predicted to be involved in secondary metabolism are also important. Springer International Publishing 2015-01-30 2015 /pmc/articles/PMC4495286/ /pubmed/26166901 http://dx.doi.org/10.1007/s11104-015-2389-5 Text en © The Author(s) 2015 https://creativecommons.org/licenses/by/4.0/ Open Access This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. |
spellingShingle | Regular Article Garcia-Fraile, Paula Seaman, Jonathan C. Karunakaran, Ramakrishnan Edwards, Anne Poole, Philip S. Downie, J. Allan Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere |
title | Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere |
title_full | Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere |
title_fullStr | Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere |
title_full_unstemmed | Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere |
title_short | Arabinose and protocatechuate catabolism genes are important for growth of Rhizobium leguminosarum biovar viciae in the pea rhizosphere |
title_sort | arabinose and protocatechuate catabolism genes are important for growth of rhizobium leguminosarum biovar viciae in the pea rhizosphere |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4495286/ https://www.ncbi.nlm.nih.gov/pubmed/26166901 http://dx.doi.org/10.1007/s11104-015-2389-5 |
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