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Oxidative stress markers in saliva and periodontal disease status: modulation during pregnancy and postpartum

BACKGROUND: Periodontal diseases may affect local and systemic inflammation, and reactive oxygen species (ROS) levels. This systemic health burden could compromise the outcome of pregnancy in expectant mothers. The aim of the present study was to evaluate oxidative stress markers, including glutathi...

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Autores principales: Gümüş, Pınar, Emingil, Gülnur, Öztürk, Veli-Özgen, Belibasakis, Georgios N., Bostanci, Nagihan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4495776/
https://www.ncbi.nlm.nih.gov/pubmed/26152310
http://dx.doi.org/10.1186/s12879-015-1003-z
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author Gümüş, Pınar
Emingil, Gülnur
Öztürk, Veli-Özgen
Belibasakis, Georgios N.
Bostanci, Nagihan
author_facet Gümüş, Pınar
Emingil, Gülnur
Öztürk, Veli-Özgen
Belibasakis, Georgios N.
Bostanci, Nagihan
author_sort Gümüş, Pınar
collection PubMed
description BACKGROUND: Periodontal diseases may affect local and systemic inflammation, and reactive oxygen species (ROS) levels. This systemic health burden could compromise the outcome of pregnancy in expectant mothers. The aim of the present study was to evaluate oxidative stress markers, including glutathione peroxidase (GPx), thiobarbituric acid-reactive substances (TBARS) and 8-hydroxy-2’-deoxyguanosine (8-OHdG), and total bacterial loads in the saliva of pregnant and postpartum women, and to investigate their association with periodontal disease severity. METHODS: A total of 187 women were originally recruited for this case–control study, assigned to the following groups a) pregnant group, b) postpartum group: the pregnant group re-evaluated 6 months after giving birth, c) control group: systemically healthy and non-pregnant women. The levels of the studied oxidative stress markers in saliva were measured by commercially available kits. RESULTS: The levels of salivary 8-OHdG were significantly elevated in the pregnant, compared with the control group. Although salivary 8-OHdG levels slightly decreased after giving birth (postpartum group), the difference did not reach significance. In contrast, the activity of antioxidant enzyme GPx in saliva was significantly lower in the pregnant than the control group. Although no differences in lipid peroxidation (represented by TBARS) were observed between the pregnant and control groups, after giving birth TBARS levels were significantly lowered. Only in the postpartum and control groups did clinical measurements of periodontal disease severity correlate with oxidative stress markers. Interestingly, there were no such correlations with TBARS in the pregnant and postpartum groups. CONCLUSIONS: The present study shows changes in the oxidant/antioxidant balance in saliva during pregnancy and after birth, which may be affected by periodontal health status in the latter case. Whether this is associated with adverse pregnancy outcomes, or not, remains to be elucidated. Early identification of ROS markers in saliva may be of clinical value in the periodontal management of pregnant women.
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spelling pubmed-44957762015-07-09 Oxidative stress markers in saliva and periodontal disease status: modulation during pregnancy and postpartum Gümüş, Pınar Emingil, Gülnur Öztürk, Veli-Özgen Belibasakis, Georgios N. Bostanci, Nagihan BMC Infect Dis Research Article BACKGROUND: Periodontal diseases may affect local and systemic inflammation, and reactive oxygen species (ROS) levels. This systemic health burden could compromise the outcome of pregnancy in expectant mothers. The aim of the present study was to evaluate oxidative stress markers, including glutathione peroxidase (GPx), thiobarbituric acid-reactive substances (TBARS) and 8-hydroxy-2’-deoxyguanosine (8-OHdG), and total bacterial loads in the saliva of pregnant and postpartum women, and to investigate their association with periodontal disease severity. METHODS: A total of 187 women were originally recruited for this case–control study, assigned to the following groups a) pregnant group, b) postpartum group: the pregnant group re-evaluated 6 months after giving birth, c) control group: systemically healthy and non-pregnant women. The levels of the studied oxidative stress markers in saliva were measured by commercially available kits. RESULTS: The levels of salivary 8-OHdG were significantly elevated in the pregnant, compared with the control group. Although salivary 8-OHdG levels slightly decreased after giving birth (postpartum group), the difference did not reach significance. In contrast, the activity of antioxidant enzyme GPx in saliva was significantly lower in the pregnant than the control group. Although no differences in lipid peroxidation (represented by TBARS) were observed between the pregnant and control groups, after giving birth TBARS levels were significantly lowered. Only in the postpartum and control groups did clinical measurements of periodontal disease severity correlate with oxidative stress markers. Interestingly, there were no such correlations with TBARS in the pregnant and postpartum groups. CONCLUSIONS: The present study shows changes in the oxidant/antioxidant balance in saliva during pregnancy and after birth, which may be affected by periodontal health status in the latter case. Whether this is associated with adverse pregnancy outcomes, or not, remains to be elucidated. Early identification of ROS markers in saliva may be of clinical value in the periodontal management of pregnant women. BioMed Central 2015-07-08 /pmc/articles/PMC4495776/ /pubmed/26152310 http://dx.doi.org/10.1186/s12879-015-1003-z Text en © Gümüş et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Gümüş, Pınar
Emingil, Gülnur
Öztürk, Veli-Özgen
Belibasakis, Georgios N.
Bostanci, Nagihan
Oxidative stress markers in saliva and periodontal disease status: modulation during pregnancy and postpartum
title Oxidative stress markers in saliva and periodontal disease status: modulation during pregnancy and postpartum
title_full Oxidative stress markers in saliva and periodontal disease status: modulation during pregnancy and postpartum
title_fullStr Oxidative stress markers in saliva and periodontal disease status: modulation during pregnancy and postpartum
title_full_unstemmed Oxidative stress markers in saliva and periodontal disease status: modulation during pregnancy and postpartum
title_short Oxidative stress markers in saliva and periodontal disease status: modulation during pregnancy and postpartum
title_sort oxidative stress markers in saliva and periodontal disease status: modulation during pregnancy and postpartum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4495776/
https://www.ncbi.nlm.nih.gov/pubmed/26152310
http://dx.doi.org/10.1186/s12879-015-1003-z
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