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Prognostic and predictive values of long non-coding RNA LINC00472 in breast cancer
LINC00472 is a novel long intergenic non-coding RNA. We evaluated LINC00472 expression in breast tumor samples using RT-qPCR, performed a meta-analysis of over 20 microarray datasets from the Gene Expression Omnibus (GEO) database, and investigated the effect of LINC00472 expression on cell prolifer...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496168/ https://www.ncbi.nlm.nih.gov/pubmed/25865225 |
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author | Shen, Yi Katsaros, Dionyssios Loo, Lenora W. M. Hernandez, Brenda Y. Chong, Clayton Canuto, Emilie Marion Biglia, Nicoletta Lu, Lingeng Risch, Harvey Chu, Wen-Ming Yu, Herbert |
author_facet | Shen, Yi Katsaros, Dionyssios Loo, Lenora W. M. Hernandez, Brenda Y. Chong, Clayton Canuto, Emilie Marion Biglia, Nicoletta Lu, Lingeng Risch, Harvey Chu, Wen-Ming Yu, Herbert |
author_sort | Shen, Yi |
collection | PubMed |
description | LINC00472 is a novel long intergenic non-coding RNA. We evaluated LINC00472 expression in breast tumor samples using RT-qPCR, performed a meta-analysis of over 20 microarray datasets from the Gene Expression Omnibus (GEO) database, and investigated the effect of LINC00472 expression on cell proliferation and migration in breast cancer cells transfected with a LINC00472-expressing vector. Our qPCR results showed that high LINC00472 expression was associated with less aggressive breast tumors and more favorable disease outcomes. Patients with high expression of LINC00472 had significantly reduced risk of relapse and death compared to those with low expression. Patients with high LINC00472 expression also had better responses to adjuvant chemo- or hormonal therapy than did patients with low expression. Results of meta-analysis on multiple studies from the GEO database were in agreement with the findings of our study. High LINC00472 was also associated with favorable molecular subtypes, Luminal A or normal-like tumors. Cell culture experiments showed that up-regulation of LINC00472 expression could suppress breast cancer cell proliferation and migration. Collectively, our clinical and in vitro studies suggest that LINC00472 is a tumor suppressor in breast cancer. Evaluating this long non-coding RNA in breast tumors may have prognostic and predictive value in the clinical management of breast cancer. |
format | Online Article Text |
id | pubmed-4496168 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-44961682015-07-10 Prognostic and predictive values of long non-coding RNA LINC00472 in breast cancer Shen, Yi Katsaros, Dionyssios Loo, Lenora W. M. Hernandez, Brenda Y. Chong, Clayton Canuto, Emilie Marion Biglia, Nicoletta Lu, Lingeng Risch, Harvey Chu, Wen-Ming Yu, Herbert Oncotarget Research Paper LINC00472 is a novel long intergenic non-coding RNA. We evaluated LINC00472 expression in breast tumor samples using RT-qPCR, performed a meta-analysis of over 20 microarray datasets from the Gene Expression Omnibus (GEO) database, and investigated the effect of LINC00472 expression on cell proliferation and migration in breast cancer cells transfected with a LINC00472-expressing vector. Our qPCR results showed that high LINC00472 expression was associated with less aggressive breast tumors and more favorable disease outcomes. Patients with high expression of LINC00472 had significantly reduced risk of relapse and death compared to those with low expression. Patients with high LINC00472 expression also had better responses to adjuvant chemo- or hormonal therapy than did patients with low expression. Results of meta-analysis on multiple studies from the GEO database were in agreement with the findings of our study. High LINC00472 was also associated with favorable molecular subtypes, Luminal A or normal-like tumors. Cell culture experiments showed that up-regulation of LINC00472 expression could suppress breast cancer cell proliferation and migration. Collectively, our clinical and in vitro studies suggest that LINC00472 is a tumor suppressor in breast cancer. Evaluating this long non-coding RNA in breast tumors may have prognostic and predictive value in the clinical management of breast cancer. Impact Journals LLC 2015-03-25 /pmc/articles/PMC4496168/ /pubmed/25865225 Text en Copyright: © 2015 Shen et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Shen, Yi Katsaros, Dionyssios Loo, Lenora W. M. Hernandez, Brenda Y. Chong, Clayton Canuto, Emilie Marion Biglia, Nicoletta Lu, Lingeng Risch, Harvey Chu, Wen-Ming Yu, Herbert Prognostic and predictive values of long non-coding RNA LINC00472 in breast cancer |
title | Prognostic and predictive values of long non-coding RNA LINC00472 in breast cancer |
title_full | Prognostic and predictive values of long non-coding RNA LINC00472 in breast cancer |
title_fullStr | Prognostic and predictive values of long non-coding RNA LINC00472 in breast cancer |
title_full_unstemmed | Prognostic and predictive values of long non-coding RNA LINC00472 in breast cancer |
title_short | Prognostic and predictive values of long non-coding RNA LINC00472 in breast cancer |
title_sort | prognostic and predictive values of long non-coding rna linc00472 in breast cancer |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496168/ https://www.ncbi.nlm.nih.gov/pubmed/25865225 |
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