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The long non-coding RNA HNF1A-AS1 regulates proliferation and metastasis in lung adenocarcinoma

Long noncoding RNAs (lncRNAs) have emerged as key regulators of tumor development and progression. The lncRNA HNF1A-antisense 1 (HNF1A-AS1) is a 2455-bp transcript on chromosome 12 with a potential oncogenic role in esophageal adenocarcinoma. Nevertheless, current understanding of the involvement of...

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Autores principales: Wu, Ying, Liu, Hongbing, Shi, Xuefei, Yao, Yanwen, Yang, Wen, Song, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Impact Journals LLC 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496209/
https://www.ncbi.nlm.nih.gov/pubmed/25863539
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author Wu, Ying
Liu, Hongbing
Shi, Xuefei
Yao, Yanwen
Yang, Wen
Song, Yong
author_facet Wu, Ying
Liu, Hongbing
Shi, Xuefei
Yao, Yanwen
Yang, Wen
Song, Yong
author_sort Wu, Ying
collection PubMed
description Long noncoding RNAs (lncRNAs) have emerged as key regulators of tumor development and progression. The lncRNA HNF1A-antisense 1 (HNF1A-AS1) is a 2455-bp transcript on chromosome 12 with a potential oncogenic role in esophageal adenocarcinoma. Nevertheless, current understanding of the involvement of HNF1A-AS1 in lung adenocarcinoma tumorigenesis remains limited. In this study, we analyzed the roles of HNF1A-AS1 in 40 lung adenocarcinoma tissues and five lung cancer cell lines. Our results showed that HNF1A-AS1 was significantly up-regulated in lung adenocarcinoma tissues compared with corresponding non-tumor tissues, and its expression level was significantly correlated with TNM stage, tumor size, and lymph node metastasis. The UCSC Cancer Genomics Browser's Kaplan-Meier plot suggested that patients in the high HNF1A-AS1 expression subgroup experienced worse overall survival compared to the low expression subgroup. Moreover, HNF1A-AS1 was determined to promote tumor proliferation and metastasis, both in vitro and in vivo, by regulating cyclin D1, E-cadherin, N-cadherin and β-catenin expression. In addition, the binding of HNF1A-AS1 to DNMT1 may explain its regulation of E-cadherin. In conclusions, we demonstrated that increased HNF1A-AS1 expression could regulate cell proliferation and metastasis and identified it as a poor prognostic biomarker in lung adenocarcinoma.
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spelling pubmed-44962092015-07-10 The long non-coding RNA HNF1A-AS1 regulates proliferation and metastasis in lung adenocarcinoma Wu, Ying Liu, Hongbing Shi, Xuefei Yao, Yanwen Yang, Wen Song, Yong Oncotarget Research Paper Long noncoding RNAs (lncRNAs) have emerged as key regulators of tumor development and progression. The lncRNA HNF1A-antisense 1 (HNF1A-AS1) is a 2455-bp transcript on chromosome 12 with a potential oncogenic role in esophageal adenocarcinoma. Nevertheless, current understanding of the involvement of HNF1A-AS1 in lung adenocarcinoma tumorigenesis remains limited. In this study, we analyzed the roles of HNF1A-AS1 in 40 lung adenocarcinoma tissues and five lung cancer cell lines. Our results showed that HNF1A-AS1 was significantly up-regulated in lung adenocarcinoma tissues compared with corresponding non-tumor tissues, and its expression level was significantly correlated with TNM stage, tumor size, and lymph node metastasis. The UCSC Cancer Genomics Browser's Kaplan-Meier plot suggested that patients in the high HNF1A-AS1 expression subgroup experienced worse overall survival compared to the low expression subgroup. Moreover, HNF1A-AS1 was determined to promote tumor proliferation and metastasis, both in vitro and in vivo, by regulating cyclin D1, E-cadherin, N-cadherin and β-catenin expression. In addition, the binding of HNF1A-AS1 to DNMT1 may explain its regulation of E-cadherin. In conclusions, we demonstrated that increased HNF1A-AS1 expression could regulate cell proliferation and metastasis and identified it as a poor prognostic biomarker in lung adenocarcinoma. Impact Journals LLC 2015-03-26 /pmc/articles/PMC4496209/ /pubmed/25863539 Text en Copyright: © 2015 Wu et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Paper
Wu, Ying
Liu, Hongbing
Shi, Xuefei
Yao, Yanwen
Yang, Wen
Song, Yong
The long non-coding RNA HNF1A-AS1 regulates proliferation and metastasis in lung adenocarcinoma
title The long non-coding RNA HNF1A-AS1 regulates proliferation and metastasis in lung adenocarcinoma
title_full The long non-coding RNA HNF1A-AS1 regulates proliferation and metastasis in lung adenocarcinoma
title_fullStr The long non-coding RNA HNF1A-AS1 regulates proliferation and metastasis in lung adenocarcinoma
title_full_unstemmed The long non-coding RNA HNF1A-AS1 regulates proliferation and metastasis in lung adenocarcinoma
title_short The long non-coding RNA HNF1A-AS1 regulates proliferation and metastasis in lung adenocarcinoma
title_sort long non-coding rna hnf1a-as1 regulates proliferation and metastasis in lung adenocarcinoma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496209/
https://www.ncbi.nlm.nih.gov/pubmed/25863539
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