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XPC inhibits NSCLC cell proliferation and migration by enhancing E-Cadherin expression
Xeroderma pigmentosum complementation group C (XPC) protein is an important DNA damage recognition factor in nucleotide excision repair. Deletion of XPC is associated with early stages of human lung carcinogenesis, and reduced XPC mRNA levels predict poor patient outcome for non-small cell lung canc...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Impact Journals LLC
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496340/ https://www.ncbi.nlm.nih.gov/pubmed/25871391 |
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author | Cui, Tiantian Srivastava, Amit Kumar Han, Chunhua Yang, Linlin Zhao, Ran Zou, Ning Qu, Meihua Duan, Wenrui Zhang, Xiaoli Wang, Qi-En |
author_facet | Cui, Tiantian Srivastava, Amit Kumar Han, Chunhua Yang, Linlin Zhao, Ran Zou, Ning Qu, Meihua Duan, Wenrui Zhang, Xiaoli Wang, Qi-En |
author_sort | Cui, Tiantian |
collection | PubMed |
description | Xeroderma pigmentosum complementation group C (XPC) protein is an important DNA damage recognition factor in nucleotide excision repair. Deletion of XPC is associated with early stages of human lung carcinogenesis, and reduced XPC mRNA levels predict poor patient outcome for non-small cell lung cancer (NSCLC). However, the mechanisms linking loss of XPC expression and poor prognosis in lung cancer are still unclear. Here, we report evidence that XPC silencing drives proliferation and migration of NSCLC cells by down-regulating E-Cadherin. XPC knockdown enhanced proliferation and migration while decreasing E-Cadherin expression in NSCLC cells with an epithelial phenotype. Restoration of E-Cadherin in these cells suppressed XPC knockdown-induced cell growth both in vitro and in vivo. Mechanistic studies showed that the loss of XPC repressed E-Cadherin expression by activating the ERK pathway and upregulating Snail expression. Our findings indicate that XPC silencing-induced reduction of E-Cadherin expression contributes, at least in part, to the poor outcome of NSCLC patients with low XPC expression. |
format | Online Article Text |
id | pubmed-4496340 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Impact Journals LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-44963402015-07-15 XPC inhibits NSCLC cell proliferation and migration by enhancing E-Cadherin expression Cui, Tiantian Srivastava, Amit Kumar Han, Chunhua Yang, Linlin Zhao, Ran Zou, Ning Qu, Meihua Duan, Wenrui Zhang, Xiaoli Wang, Qi-En Oncotarget Research Paper Xeroderma pigmentosum complementation group C (XPC) protein is an important DNA damage recognition factor in nucleotide excision repair. Deletion of XPC is associated with early stages of human lung carcinogenesis, and reduced XPC mRNA levels predict poor patient outcome for non-small cell lung cancer (NSCLC). However, the mechanisms linking loss of XPC expression and poor prognosis in lung cancer are still unclear. Here, we report evidence that XPC silencing drives proliferation and migration of NSCLC cells by down-regulating E-Cadherin. XPC knockdown enhanced proliferation and migration while decreasing E-Cadherin expression in NSCLC cells with an epithelial phenotype. Restoration of E-Cadherin in these cells suppressed XPC knockdown-induced cell growth both in vitro and in vivo. Mechanistic studies showed that the loss of XPC repressed E-Cadherin expression by activating the ERK pathway and upregulating Snail expression. Our findings indicate that XPC silencing-induced reduction of E-Cadherin expression contributes, at least in part, to the poor outcome of NSCLC patients with low XPC expression. Impact Journals LLC 2015-03-12 /pmc/articles/PMC4496340/ /pubmed/25871391 Text en Copyright: © 2015 Cui et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Paper Cui, Tiantian Srivastava, Amit Kumar Han, Chunhua Yang, Linlin Zhao, Ran Zou, Ning Qu, Meihua Duan, Wenrui Zhang, Xiaoli Wang, Qi-En XPC inhibits NSCLC cell proliferation and migration by enhancing E-Cadherin expression |
title | XPC inhibits NSCLC cell proliferation and migration by enhancing E-Cadherin expression |
title_full | XPC inhibits NSCLC cell proliferation and migration by enhancing E-Cadherin expression |
title_fullStr | XPC inhibits NSCLC cell proliferation and migration by enhancing E-Cadherin expression |
title_full_unstemmed | XPC inhibits NSCLC cell proliferation and migration by enhancing E-Cadherin expression |
title_short | XPC inhibits NSCLC cell proliferation and migration by enhancing E-Cadherin expression |
title_sort | xpc inhibits nsclc cell proliferation and migration by enhancing e-cadherin expression |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496340/ https://www.ncbi.nlm.nih.gov/pubmed/25871391 |
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