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Dysregulation of the miR-34a-SIRT1 axis inhibits breast cancer stemness
Enforced expression of miR-34a eliminates cancer stem cells in some malignant tumors. Sirtuin-1 (SIRT1) is a direct target of miR-34a. Here we found low levels of miR-34a and high levels of SIRT1 in CD44+/CD24− breast cancer stem cells (BCSCs). MiR-34a overexpression and knockdown of SIRT1 decreased...
| Autores principales: | , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Impact Journals LLC
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496365/ https://www.ncbi.nlm.nih.gov/pubmed/25826085 |
| _version_ | 1782380388083040256 |
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| author | Ma, Wei Xiao, Gary Guishan Mao, Jun Lu, Ying Song, Bo Wang, Lihui Fan, Shujun Fan, Panhong Hou, Zhenhuan Li, Jiazhi Yu, Xiaotang Wang, Bo Wang, Huan Wang, Honghai Xu, Fei Li, Yan Liu, Qiang Li, Lianhong |
| author_facet | Ma, Wei Xiao, Gary Guishan Mao, Jun Lu, Ying Song, Bo Wang, Lihui Fan, Shujun Fan, Panhong Hou, Zhenhuan Li, Jiazhi Yu, Xiaotang Wang, Bo Wang, Huan Wang, Honghai Xu, Fei Li, Yan Liu, Qiang Li, Lianhong |
| author_sort | Ma, Wei |
| collection | PubMed |
| description | Enforced expression of miR-34a eliminates cancer stem cells in some malignant tumors. Sirtuin-1 (SIRT1) is a direct target of miR-34a. Here we found low levels of miR-34a and high levels of SIRT1 in CD44+/CD24− breast cancer stem cells (BCSCs). MiR-34a overexpression and knockdown of SIRT1 decreased proportion of BSCSs and mammosphere formation. Expression of CSC markers, ALDH1, BMI1 and Nanog was decreased. In nude mice xenografts, stable expression of miR-34a and silencing of SIRT1 reduced tumor burden. Taken together, our results demonstrated that miR-34a inhibits proliferative potential of BCSCs in vitro and in vivo, at least partially by downregulating SIRT1. The miR-34a-SIRT1 axis may play role in self-renewal of BCSCs. |
| format | Online Article Text |
| id | pubmed-4496365 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2015 |
| publisher | Impact Journals LLC |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-44963652015-07-15 Dysregulation of the miR-34a-SIRT1 axis inhibits breast cancer stemness Ma, Wei Xiao, Gary Guishan Mao, Jun Lu, Ying Song, Bo Wang, Lihui Fan, Shujun Fan, Panhong Hou, Zhenhuan Li, Jiazhi Yu, Xiaotang Wang, Bo Wang, Huan Wang, Honghai Xu, Fei Li, Yan Liu, Qiang Li, Lianhong Oncotarget Research Paper Enforced expression of miR-34a eliminates cancer stem cells in some malignant tumors. Sirtuin-1 (SIRT1) is a direct target of miR-34a. Here we found low levels of miR-34a and high levels of SIRT1 in CD44+/CD24− breast cancer stem cells (BCSCs). MiR-34a overexpression and knockdown of SIRT1 decreased proportion of BSCSs and mammosphere formation. Expression of CSC markers, ALDH1, BMI1 and Nanog was decreased. In nude mice xenografts, stable expression of miR-34a and silencing of SIRT1 reduced tumor burden. Taken together, our results demonstrated that miR-34a inhibits proliferative potential of BCSCs in vitro and in vivo, at least partially by downregulating SIRT1. The miR-34a-SIRT1 axis may play role in self-renewal of BCSCs. Impact Journals LLC 2015-03-18 /pmc/articles/PMC4496365/ /pubmed/25826085 Text en Copyright: © 2015 Ma et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
| spellingShingle | Research Paper Ma, Wei Xiao, Gary Guishan Mao, Jun Lu, Ying Song, Bo Wang, Lihui Fan, Shujun Fan, Panhong Hou, Zhenhuan Li, Jiazhi Yu, Xiaotang Wang, Bo Wang, Huan Wang, Honghai Xu, Fei Li, Yan Liu, Qiang Li, Lianhong Dysregulation of the miR-34a-SIRT1 axis inhibits breast cancer stemness |
| title | Dysregulation of the miR-34a-SIRT1 axis inhibits breast cancer stemness |
| title_full | Dysregulation of the miR-34a-SIRT1 axis inhibits breast cancer stemness |
| title_fullStr | Dysregulation of the miR-34a-SIRT1 axis inhibits breast cancer stemness |
| title_full_unstemmed | Dysregulation of the miR-34a-SIRT1 axis inhibits breast cancer stemness |
| title_short | Dysregulation of the miR-34a-SIRT1 axis inhibits breast cancer stemness |
| title_sort | dysregulation of the mir-34a-sirt1 axis inhibits breast cancer stemness |
| topic | Research Paper |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496365/ https://www.ncbi.nlm.nih.gov/pubmed/25826085 |
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