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Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection
Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large grou...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496563/ https://www.ncbi.nlm.nih.gov/pubmed/26217367 http://dx.doi.org/10.3389/fpls.2015.00517 |
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author | Curto, Miguel Krajinski, Franziska Schlereth, Armin Rubiales, Diego |
author_facet | Curto, Miguel Krajinski, Franziska Schlereth, Armin Rubiales, Diego |
author_sort | Curto, Miguel |
collection | PubMed |
description | Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense. Using high-throughput quantitative real-time PCR (qPCR) technology more than 1000 Medicago truncatula TFs were screened in a pair of susceptible and resistant genotypes of M. truncatula after 4 h of Erysiphe pisi infection. Seventy nine TF genes, belonging to 33 families showed a significant transcriptional change in response to E. pisi infection. Forty eight TF genes were differentially expressed in the resistant genotypes compared to the susceptible one in response to E. pisi infection, including pathogenesis-related transcriptional factors, AP2/EREBP (APETALA2/ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS), WRKY (highly conserved WRKYGQK amino-acid sequence), MYB (Myeloblastoma), homeodomain (HD) and zinc finger C(2)C(2) (CYS(2)-CYS(2)), C(2)H(2), (CYS(2)-HIS(2)), LIM (Lin-11, Isl-1, Mec-3) gene families, which are involved in known defense responses. Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi. |
format | Online Article Text |
id | pubmed-4496563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-44965632015-07-27 Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection Curto, Miguel Krajinski, Franziska Schlereth, Armin Rubiales, Diego Front Plant Sci Plant Science Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense. Using high-throughput quantitative real-time PCR (qPCR) technology more than 1000 Medicago truncatula TFs were screened in a pair of susceptible and resistant genotypes of M. truncatula after 4 h of Erysiphe pisi infection. Seventy nine TF genes, belonging to 33 families showed a significant transcriptional change in response to E. pisi infection. Forty eight TF genes were differentially expressed in the resistant genotypes compared to the susceptible one in response to E. pisi infection, including pathogenesis-related transcriptional factors, AP2/EREBP (APETALA2/ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS), WRKY (highly conserved WRKYGQK amino-acid sequence), MYB (Myeloblastoma), homeodomain (HD) and zinc finger C(2)C(2) (CYS(2)-CYS(2)), C(2)H(2), (CYS(2)-HIS(2)), LIM (Lin-11, Isl-1, Mec-3) gene families, which are involved in known defense responses. Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi. Frontiers Media S.A. 2015-07-09 /pmc/articles/PMC4496563/ /pubmed/26217367 http://dx.doi.org/10.3389/fpls.2015.00517 Text en Copyright © 2015 Curto, Krajinski, Schlereth and Rubiales. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Curto, Miguel Krajinski, Franziska Schlereth, Armin Rubiales, Diego Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection |
title | Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection |
title_full | Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection |
title_fullStr | Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection |
title_full_unstemmed | Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection |
title_short | Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection |
title_sort | transcriptional profiling of medicago truncatula during erysiphe pisi infection |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496563/ https://www.ncbi.nlm.nih.gov/pubmed/26217367 http://dx.doi.org/10.3389/fpls.2015.00517 |
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