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Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection

Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large grou...

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Autores principales: Curto, Miguel, Krajinski, Franziska, Schlereth, Armin, Rubiales, Diego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496563/
https://www.ncbi.nlm.nih.gov/pubmed/26217367
http://dx.doi.org/10.3389/fpls.2015.00517
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author Curto, Miguel
Krajinski, Franziska
Schlereth, Armin
Rubiales, Diego
author_facet Curto, Miguel
Krajinski, Franziska
Schlereth, Armin
Rubiales, Diego
author_sort Curto, Miguel
collection PubMed
description Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense. Using high-throughput quantitative real-time PCR (qPCR) technology more than 1000 Medicago truncatula TFs were screened in a pair of susceptible and resistant genotypes of M. truncatula after 4 h of Erysiphe pisi infection. Seventy nine TF genes, belonging to 33 families showed a significant transcriptional change in response to E. pisi infection. Forty eight TF genes were differentially expressed in the resistant genotypes compared to the susceptible one in response to E. pisi infection, including pathogenesis-related transcriptional factors, AP2/EREBP (APETALA2/ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS), WRKY (highly conserved WRKYGQK amino-acid sequence), MYB (Myeloblastoma), homeodomain (HD) and zinc finger C(2)C(2) (CYS(2)-CYS(2)), C(2)H(2), (CYS(2)-HIS(2)), LIM (Lin-11, Isl-1, Mec-3) gene families, which are involved in known defense responses. Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi.
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spelling pubmed-44965632015-07-27 Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection Curto, Miguel Krajinski, Franziska Schlereth, Armin Rubiales, Diego Front Plant Sci Plant Science Resistance to powdery mildew has been studied in a number of plant species, yet the molecular mechanisms remain largely unknown. Transcription factors (TFs) play a critical role in the plant defense response by regulating the transcriptional machinery which coordinates the expression of a large group of genes involved in plant defense. Using high-throughput quantitative real-time PCR (qPCR) technology more than 1000 Medicago truncatula TFs were screened in a pair of susceptible and resistant genotypes of M. truncatula after 4 h of Erysiphe pisi infection. Seventy nine TF genes, belonging to 33 families showed a significant transcriptional change in response to E. pisi infection. Forty eight TF genes were differentially expressed in the resistant genotypes compared to the susceptible one in response to E. pisi infection, including pathogenesis-related transcriptional factors, AP2/EREBP (APETALA2/ETHYLENE-RESPONSIVE ELEMENT BINDING FACTORS), WRKY (highly conserved WRKYGQK amino-acid sequence), MYB (Myeloblastoma), homeodomain (HD) and zinc finger C(2)C(2) (CYS(2)-CYS(2)), C(2)H(2), (CYS(2)-HIS(2)), LIM (Lin-11, Isl-1, Mec-3) gene families, which are involved in known defense responses. Our results suggest that these TF genes are among the E. pisi responsive genes in resistant M. truncatula that may constitute a regulatory network which controls the transcriptional changes in defense genes involved in resistance to E. pisi. Frontiers Media S.A. 2015-07-09 /pmc/articles/PMC4496563/ /pubmed/26217367 http://dx.doi.org/10.3389/fpls.2015.00517 Text en Copyright © 2015 Curto, Krajinski, Schlereth and Rubiales. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Curto, Miguel
Krajinski, Franziska
Schlereth, Armin
Rubiales, Diego
Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection
title Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection
title_full Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection
title_fullStr Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection
title_full_unstemmed Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection
title_short Transcriptional profiling of Medicago truncatula during Erysiphe pisi infection
title_sort transcriptional profiling of medicago truncatula during erysiphe pisi infection
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496563/
https://www.ncbi.nlm.nih.gov/pubmed/26217367
http://dx.doi.org/10.3389/fpls.2015.00517
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