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Pituitary Adenylate Cyclase-Activating Polypeptide Reverses Ammonium Metavanadate-Induced Airway Hyperresponsiveness in Rats

The rate of atmospheric vanadium is constantly increasing due to fossil fuel combustion. This environmental pollution favours vanadium exposure in particular to its vanadate form, causing occupational bronchial asthma and bronchitis. Based on the well admitted bronchodilator properties of the pituit...

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Autores principales: Tlili, Mounira, Rouatbi, Sonia, Sriha, Badreddine, Ben Rhouma, Khémais, Sakly, Mohsen, Vaudry, David, Wurtz, Olivier, Tebourbi, Olfa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496651/
https://www.ncbi.nlm.nih.gov/pubmed/26199679
http://dx.doi.org/10.1155/2015/787561
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author Tlili, Mounira
Rouatbi, Sonia
Sriha, Badreddine
Ben Rhouma, Khémais
Sakly, Mohsen
Vaudry, David
Wurtz, Olivier
Tebourbi, Olfa
author_facet Tlili, Mounira
Rouatbi, Sonia
Sriha, Badreddine
Ben Rhouma, Khémais
Sakly, Mohsen
Vaudry, David
Wurtz, Olivier
Tebourbi, Olfa
author_sort Tlili, Mounira
collection PubMed
description The rate of atmospheric vanadium is constantly increasing due to fossil fuel combustion. This environmental pollution favours vanadium exposure in particular to its vanadate form, causing occupational bronchial asthma and bronchitis. Based on the well admitted bronchodilator properties of the pituitary adenylate cyclase-activating polypeptide (PACAP), we investigated the ability of this neuropeptide to reverse the vanadate-induced airway hyperresponsiveness in rats. Exposure to ammonium metavanadate aerosols (5 mg/m(3)/h) for 15 minutes induced 4 hours later an array of pathophysiological events, including increase of bronchial resistance and histological alterations, activation of proinflammatory alveolar macrophages, and increased oxidative stress status. Powerfully, PACAP inhalation (0.1 mM) for 10 minutes alleviated many of these deleterious effects as demonstrated by a decrease of bronchial resistance and histological restoration. PACAP reduced the level of expression of mRNA encoding inflammatory chemokines (MIP-1α, MIP-2, and KC) and cytokines (IL-1α and TNF-α) in alveolar macrophages and improved the antioxidant status. PACAP reverses the vanadate-induced airway hyperresponsiveness not only through its bronchodilator activity but also by counteracting the proinflammatory and prooxidative effects of the metal. Then, the development of stable analogs of PACAP could represent a promising therapeutic alternative for the treatment of inflammatory respiratory disorders.
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spelling pubmed-44966512015-07-21 Pituitary Adenylate Cyclase-Activating Polypeptide Reverses Ammonium Metavanadate-Induced Airway Hyperresponsiveness in Rats Tlili, Mounira Rouatbi, Sonia Sriha, Badreddine Ben Rhouma, Khémais Sakly, Mohsen Vaudry, David Wurtz, Olivier Tebourbi, Olfa Oxid Med Cell Longev Research Article The rate of atmospheric vanadium is constantly increasing due to fossil fuel combustion. This environmental pollution favours vanadium exposure in particular to its vanadate form, causing occupational bronchial asthma and bronchitis. Based on the well admitted bronchodilator properties of the pituitary adenylate cyclase-activating polypeptide (PACAP), we investigated the ability of this neuropeptide to reverse the vanadate-induced airway hyperresponsiveness in rats. Exposure to ammonium metavanadate aerosols (5 mg/m(3)/h) for 15 minutes induced 4 hours later an array of pathophysiological events, including increase of bronchial resistance and histological alterations, activation of proinflammatory alveolar macrophages, and increased oxidative stress status. Powerfully, PACAP inhalation (0.1 mM) for 10 minutes alleviated many of these deleterious effects as demonstrated by a decrease of bronchial resistance and histological restoration. PACAP reduced the level of expression of mRNA encoding inflammatory chemokines (MIP-1α, MIP-2, and KC) and cytokines (IL-1α and TNF-α) in alveolar macrophages and improved the antioxidant status. PACAP reverses the vanadate-induced airway hyperresponsiveness not only through its bronchodilator activity but also by counteracting the proinflammatory and prooxidative effects of the metal. Then, the development of stable analogs of PACAP could represent a promising therapeutic alternative for the treatment of inflammatory respiratory disorders. Hindawi Publishing Corporation 2015 2015-06-14 /pmc/articles/PMC4496651/ /pubmed/26199679 http://dx.doi.org/10.1155/2015/787561 Text en Copyright © 2015 Mounira Tlili et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tlili, Mounira
Rouatbi, Sonia
Sriha, Badreddine
Ben Rhouma, Khémais
Sakly, Mohsen
Vaudry, David
Wurtz, Olivier
Tebourbi, Olfa
Pituitary Adenylate Cyclase-Activating Polypeptide Reverses Ammonium Metavanadate-Induced Airway Hyperresponsiveness in Rats
title Pituitary Adenylate Cyclase-Activating Polypeptide Reverses Ammonium Metavanadate-Induced Airway Hyperresponsiveness in Rats
title_full Pituitary Adenylate Cyclase-Activating Polypeptide Reverses Ammonium Metavanadate-Induced Airway Hyperresponsiveness in Rats
title_fullStr Pituitary Adenylate Cyclase-Activating Polypeptide Reverses Ammonium Metavanadate-Induced Airway Hyperresponsiveness in Rats
title_full_unstemmed Pituitary Adenylate Cyclase-Activating Polypeptide Reverses Ammonium Metavanadate-Induced Airway Hyperresponsiveness in Rats
title_short Pituitary Adenylate Cyclase-Activating Polypeptide Reverses Ammonium Metavanadate-Induced Airway Hyperresponsiveness in Rats
title_sort pituitary adenylate cyclase-activating polypeptide reverses ammonium metavanadate-induced airway hyperresponsiveness in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496651/
https://www.ncbi.nlm.nih.gov/pubmed/26199679
http://dx.doi.org/10.1155/2015/787561
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