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Structural and Biochemical Investigation of Bacteriophage N4-Encoded RNA Polymerases

Bacteriophage N4 regulates the temporal expression of its genome through the activity of three distinct RNA polymerases (RNAP). Expression of the early genes is carried out by a phage-encoded, virion-encapsidated RNAP (vRNAP) that is injected into the host at the onset of infection and transcribes t...

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Autores principales: Lenneman, Bryan R., Rothman-Denes, Lucia B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496689/
https://www.ncbi.nlm.nih.gov/pubmed/25924224
http://dx.doi.org/10.3390/biom5020647
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author Lenneman, Bryan R.
Rothman-Denes, Lucia B.
author_facet Lenneman, Bryan R.
Rothman-Denes, Lucia B.
author_sort Lenneman, Bryan R.
collection PubMed
description Bacteriophage N4 regulates the temporal expression of its genome through the activity of three distinct RNA polymerases (RNAP). Expression of the early genes is carried out by a phage-encoded, virion-encapsidated RNAP (vRNAP) that is injected into the host at the onset of infection and transcribes the early genes. These encode the components of new transcriptional machinery (N4 RNAPII and cofactors) responsible for the synthesis of middle RNAs. Both N4 RNAPs belong to the T7-like “single-subunit” family of polymerases. Herein, we describe their mechanisms of promoter recognition, regulation, and roles in the phage life cycle.
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spelling pubmed-44966892015-07-10 Structural and Biochemical Investigation of Bacteriophage N4-Encoded RNA Polymerases Lenneman, Bryan R. Rothman-Denes, Lucia B. Biomolecules Review Bacteriophage N4 regulates the temporal expression of its genome through the activity of three distinct RNA polymerases (RNAP). Expression of the early genes is carried out by a phage-encoded, virion-encapsidated RNAP (vRNAP) that is injected into the host at the onset of infection and transcribes the early genes. These encode the components of new transcriptional machinery (N4 RNAPII and cofactors) responsible for the synthesis of middle RNAs. Both N4 RNAPs belong to the T7-like “single-subunit” family of polymerases. Herein, we describe their mechanisms of promoter recognition, regulation, and roles in the phage life cycle. MDPI 2015-04-27 /pmc/articles/PMC4496689/ /pubmed/25924224 http://dx.doi.org/10.3390/biom5020647 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Lenneman, Bryan R.
Rothman-Denes, Lucia B.
Structural and Biochemical Investigation of Bacteriophage N4-Encoded RNA Polymerases
title Structural and Biochemical Investigation of Bacteriophage N4-Encoded RNA Polymerases
title_full Structural and Biochemical Investigation of Bacteriophage N4-Encoded RNA Polymerases
title_fullStr Structural and Biochemical Investigation of Bacteriophage N4-Encoded RNA Polymerases
title_full_unstemmed Structural and Biochemical Investigation of Bacteriophage N4-Encoded RNA Polymerases
title_short Structural and Biochemical Investigation of Bacteriophage N4-Encoded RNA Polymerases
title_sort structural and biochemical investigation of bacteriophage n4-encoded rna polymerases
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496689/
https://www.ncbi.nlm.nih.gov/pubmed/25924224
http://dx.doi.org/10.3390/biom5020647
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