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Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism
Many neurodegenerative diseases, including Huntington’s, Alzheimer’s and Parkinson’s diseases, are characterized by protein misfolding and aggregation. The capability of trehalose to interfere with protein misfolding and aggregation has been recently evaluated by several research groups. In the pres...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496693/ https://www.ncbi.nlm.nih.gov/pubmed/25946077 http://dx.doi.org/10.3390/biom5020724 |
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author | Ruzza, Paolo Hussain, Rohanah Biondi, Barbara Calderan, Andrea Tessari, Isabella Bubacco, Luigi Siligardi, Giuliano |
author_facet | Ruzza, Paolo Hussain, Rohanah Biondi, Barbara Calderan, Andrea Tessari, Isabella Bubacco, Luigi Siligardi, Giuliano |
author_sort | Ruzza, Paolo |
collection | PubMed |
description | Many neurodegenerative diseases, including Huntington’s, Alzheimer’s and Parkinson’s diseases, are characterized by protein misfolding and aggregation. The capability of trehalose to interfere with protein misfolding and aggregation has been recently evaluated by several research groups. In the present work, we studied, by means of synchrotron radiation circular dichroism (SRCD) spectroscopy, the dose-effect of trehalose on α-synuclein conformation and/or stability to probe the capability of this osmolyte to interfere with α-synuclein’s aggregation. Our study indicated that a low trehalose concentration stabilized α-synuclein folding much better than at high concentration by blocking in vitro α-synuclein’s polymerisation. These results suggested that trehalose could be associated with other drugs leading to a new approach for treating Parkinson’s and other brain-related diseases. |
format | Online Article Text |
id | pubmed-4496693 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-44966932015-07-10 Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism Ruzza, Paolo Hussain, Rohanah Biondi, Barbara Calderan, Andrea Tessari, Isabella Bubacco, Luigi Siligardi, Giuliano Biomolecules Article Many neurodegenerative diseases, including Huntington’s, Alzheimer’s and Parkinson’s diseases, are characterized by protein misfolding and aggregation. The capability of trehalose to interfere with protein misfolding and aggregation has been recently evaluated by several research groups. In the present work, we studied, by means of synchrotron radiation circular dichroism (SRCD) spectroscopy, the dose-effect of trehalose on α-synuclein conformation and/or stability to probe the capability of this osmolyte to interfere with α-synuclein’s aggregation. Our study indicated that a low trehalose concentration stabilized α-synuclein folding much better than at high concentration by blocking in vitro α-synuclein’s polymerisation. These results suggested that trehalose could be associated with other drugs leading to a new approach for treating Parkinson’s and other brain-related diseases. MDPI 2015-05-04 /pmc/articles/PMC4496693/ /pubmed/25946077 http://dx.doi.org/10.3390/biom5020724 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ruzza, Paolo Hussain, Rohanah Biondi, Barbara Calderan, Andrea Tessari, Isabella Bubacco, Luigi Siligardi, Giuliano Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism |
title | Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism |
title_full | Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism |
title_fullStr | Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism |
title_full_unstemmed | Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism |
title_short | Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism |
title_sort | effects of trehalose on thermodynamic properties of alpha-synuclein revealed through synchrotron radiation circular dichroism |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496693/ https://www.ncbi.nlm.nih.gov/pubmed/25946077 http://dx.doi.org/10.3390/biom5020724 |
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