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Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism

Many neurodegenerative diseases, including Huntington’s, Alzheimer’s and Parkinson’s diseases, are characterized by protein misfolding and aggregation. The capability of trehalose to interfere with protein misfolding and aggregation has been recently evaluated by several research groups. In the pres...

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Detalles Bibliográficos
Autores principales: Ruzza, Paolo, Hussain, Rohanah, Biondi, Barbara, Calderan, Andrea, Tessari, Isabella, Bubacco, Luigi, Siligardi, Giuliano
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496693/
https://www.ncbi.nlm.nih.gov/pubmed/25946077
http://dx.doi.org/10.3390/biom5020724
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author Ruzza, Paolo
Hussain, Rohanah
Biondi, Barbara
Calderan, Andrea
Tessari, Isabella
Bubacco, Luigi
Siligardi, Giuliano
author_facet Ruzza, Paolo
Hussain, Rohanah
Biondi, Barbara
Calderan, Andrea
Tessari, Isabella
Bubacco, Luigi
Siligardi, Giuliano
author_sort Ruzza, Paolo
collection PubMed
description Many neurodegenerative diseases, including Huntington’s, Alzheimer’s and Parkinson’s diseases, are characterized by protein misfolding and aggregation. The capability of trehalose to interfere with protein misfolding and aggregation has been recently evaluated by several research groups. In the present work, we studied, by means of synchrotron radiation circular dichroism (SRCD) spectroscopy, the dose-effect of trehalose on α-synuclein conformation and/or stability to probe the capability of this osmolyte to interfere with α-synuclein’s aggregation. Our study indicated that a low trehalose concentration stabilized α-synuclein folding much better than at high concentration by blocking in vitro α-synuclein’s polymerisation. These results suggested that trehalose could be associated with other drugs leading to a new approach for treating Parkinson’s and other brain-related diseases.
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spelling pubmed-44966932015-07-10 Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism Ruzza, Paolo Hussain, Rohanah Biondi, Barbara Calderan, Andrea Tessari, Isabella Bubacco, Luigi Siligardi, Giuliano Biomolecules Article Many neurodegenerative diseases, including Huntington’s, Alzheimer’s and Parkinson’s diseases, are characterized by protein misfolding and aggregation. The capability of trehalose to interfere with protein misfolding and aggregation has been recently evaluated by several research groups. In the present work, we studied, by means of synchrotron radiation circular dichroism (SRCD) spectroscopy, the dose-effect of trehalose on α-synuclein conformation and/or stability to probe the capability of this osmolyte to interfere with α-synuclein’s aggregation. Our study indicated that a low trehalose concentration stabilized α-synuclein folding much better than at high concentration by blocking in vitro α-synuclein’s polymerisation. These results suggested that trehalose could be associated with other drugs leading to a new approach for treating Parkinson’s and other brain-related diseases. MDPI 2015-05-04 /pmc/articles/PMC4496693/ /pubmed/25946077 http://dx.doi.org/10.3390/biom5020724 Text en © 2015 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ruzza, Paolo
Hussain, Rohanah
Biondi, Barbara
Calderan, Andrea
Tessari, Isabella
Bubacco, Luigi
Siligardi, Giuliano
Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism
title Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism
title_full Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism
title_fullStr Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism
title_full_unstemmed Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism
title_short Effects of Trehalose on Thermodynamic Properties of Alpha-synuclein Revealed through Synchrotron Radiation Circular Dichroism
title_sort effects of trehalose on thermodynamic properties of alpha-synuclein revealed through synchrotron radiation circular dichroism
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4496693/
https://www.ncbi.nlm.nih.gov/pubmed/25946077
http://dx.doi.org/10.3390/biom5020724
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