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Analysis of the antioxidative function of the radioprotective Japanese traditional (Kampo) medicine, hangeshashinto, in an aqueous phase

Oral mucositis (OM) is a common and painful complication of radiotherapy for head and neck cancer. Hangeshashinto (HST), a Japanese traditional medicine, is known to alleviate radiotherapy- and/or chemotherapy-induced OM; however, the detailed mechanism has not yet been clarified. The aim of the pre...

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Autores principales: Matsumoto, Chinami, Sekine-Suzuki, Emiko, Nyui, Minako, Ueno, Megumi, Nakanishi, Ikuo, Omiya, Yuji, Fukutake, Masato, Kase, Yoshio, Matsumoto, Ken-ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497396/
https://www.ncbi.nlm.nih.gov/pubmed/25883171
http://dx.doi.org/10.1093/jrr/rrv023
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author Matsumoto, Chinami
Sekine-Suzuki, Emiko
Nyui, Minako
Ueno, Megumi
Nakanishi, Ikuo
Omiya, Yuji
Fukutake, Masato
Kase, Yoshio
Matsumoto, Ken-ichiro
author_facet Matsumoto, Chinami
Sekine-Suzuki, Emiko
Nyui, Minako
Ueno, Megumi
Nakanishi, Ikuo
Omiya, Yuji
Fukutake, Masato
Kase, Yoshio
Matsumoto, Ken-ichiro
author_sort Matsumoto, Chinami
collection PubMed
description Oral mucositis (OM) is a common and painful complication of radiotherapy for head and neck cancer. Hangeshashinto (HST), a Japanese traditional medicine, is known to alleviate radiotherapy- and/or chemotherapy-induced OM; however, the detailed mechanism has not yet been clarified. The aim of the present study was to clarify the details of the antioxidative functions of HST against reactive oxygen species (ROS) produced by radiation. The hydroxyl radical (•OH)–scavenging ability and the reduction ability was simultaneously measured using a modified electron paramagnetic resonance (EPR) spin-trapping method. The superoxide (O(2)(•−))–scavenging ability was estimated by an EPR redox probing method. Water suspensions of powdered HST and of its seven constitutive crude drugs were tested. In addition, some of the main water-soluble ingredients of the crude drugs were also tested. HST was found to scavenge both •OH and O(2)(•−). Furthermore, HST was observed to reduce relatively stable nitroxyl radicals. Glycyrrhizae Radix (kanzo), Ginseng Radix (ninjin), Zizyphi Fructus (taiso) and glycyrrhizin (an ingredient of kanzo) were all found to be relatively good •OH scavengers. Scutellariae Radix (ogon) and Coptidis Rhizoma (oren) demonstrated reducing ability. In addition, acteoside and berberine chloride, which are water-soluble ingredients of ogon and oren, respectively, also demonstrated reducing ability. Oren exhibited oxidative ability at higher concentrations, which may have a function in maintaining catalytic redox action. The antioxidative function of HST probably worked via a balance of scavenging ROS, reducing stable free radicals, and some minor oxidizing activities.
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spelling pubmed-44973962015-07-10 Analysis of the antioxidative function of the radioprotective Japanese traditional (Kampo) medicine, hangeshashinto, in an aqueous phase Matsumoto, Chinami Sekine-Suzuki, Emiko Nyui, Minako Ueno, Megumi Nakanishi, Ikuo Omiya, Yuji Fukutake, Masato Kase, Yoshio Matsumoto, Ken-ichiro J Radiat Res Biology Oral mucositis (OM) is a common and painful complication of radiotherapy for head and neck cancer. Hangeshashinto (HST), a Japanese traditional medicine, is known to alleviate radiotherapy- and/or chemotherapy-induced OM; however, the detailed mechanism has not yet been clarified. The aim of the present study was to clarify the details of the antioxidative functions of HST against reactive oxygen species (ROS) produced by radiation. The hydroxyl radical (•OH)–scavenging ability and the reduction ability was simultaneously measured using a modified electron paramagnetic resonance (EPR) spin-trapping method. The superoxide (O(2)(•−))–scavenging ability was estimated by an EPR redox probing method. Water suspensions of powdered HST and of its seven constitutive crude drugs were tested. In addition, some of the main water-soluble ingredients of the crude drugs were also tested. HST was found to scavenge both •OH and O(2)(•−). Furthermore, HST was observed to reduce relatively stable nitroxyl radicals. Glycyrrhizae Radix (kanzo), Ginseng Radix (ninjin), Zizyphi Fructus (taiso) and glycyrrhizin (an ingredient of kanzo) were all found to be relatively good •OH scavengers. Scutellariae Radix (ogon) and Coptidis Rhizoma (oren) demonstrated reducing ability. In addition, acteoside and berberine chloride, which are water-soluble ingredients of ogon and oren, respectively, also demonstrated reducing ability. Oren exhibited oxidative ability at higher concentrations, which may have a function in maintaining catalytic redox action. The antioxidative function of HST probably worked via a balance of scavenging ROS, reducing stable free radicals, and some minor oxidizing activities. Oxford University Press 2015-07 2015-04-16 /pmc/articles/PMC4497396/ /pubmed/25883171 http://dx.doi.org/10.1093/jrr/rrv023 Text en © The Author 2015. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Biology
Matsumoto, Chinami
Sekine-Suzuki, Emiko
Nyui, Minako
Ueno, Megumi
Nakanishi, Ikuo
Omiya, Yuji
Fukutake, Masato
Kase, Yoshio
Matsumoto, Ken-ichiro
Analysis of the antioxidative function of the radioprotective Japanese traditional (Kampo) medicine, hangeshashinto, in an aqueous phase
title Analysis of the antioxidative function of the radioprotective Japanese traditional (Kampo) medicine, hangeshashinto, in an aqueous phase
title_full Analysis of the antioxidative function of the radioprotective Japanese traditional (Kampo) medicine, hangeshashinto, in an aqueous phase
title_fullStr Analysis of the antioxidative function of the radioprotective Japanese traditional (Kampo) medicine, hangeshashinto, in an aqueous phase
title_full_unstemmed Analysis of the antioxidative function of the radioprotective Japanese traditional (Kampo) medicine, hangeshashinto, in an aqueous phase
title_short Analysis of the antioxidative function of the radioprotective Japanese traditional (Kampo) medicine, hangeshashinto, in an aqueous phase
title_sort analysis of the antioxidative function of the radioprotective japanese traditional (kampo) medicine, hangeshashinto, in an aqueous phase
topic Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497396/
https://www.ncbi.nlm.nih.gov/pubmed/25883171
http://dx.doi.org/10.1093/jrr/rrv023
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