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Host Specificity of Ovine Bordetella parapertussis and the Role of Complement
The classical bordetellae are comprised of three subspecies that differ from broad to very limited host specificity. Although several lineages appear to have specialized to particular host species, most retain the ability to colonize and grow in mice, providing a powerful common experimental model t...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497623/ https://www.ncbi.nlm.nih.gov/pubmed/26158540 http://dx.doi.org/10.1371/journal.pone.0130964 |
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author | Hester, Sara E. Goodfield, Laura L. Park, Jihye Feaga, Heather A. Ivanov, Yury V. Bendor, Liron Taylor, Dawn L. Harvill, Eric T. |
author_facet | Hester, Sara E. Goodfield, Laura L. Park, Jihye Feaga, Heather A. Ivanov, Yury V. Bendor, Liron Taylor, Dawn L. Harvill, Eric T. |
author_sort | Hester, Sara E. |
collection | PubMed |
description | The classical bordetellae are comprised of three subspecies that differ from broad to very limited host specificity. Although several lineages appear to have specialized to particular host species, most retain the ability to colonize and grow in mice, providing a powerful common experimental model to study their differences. One of the subspecies, Bordetella parapertussis, is composed of two distinct clades that have specialized to different hosts: one to humans (Bpp(hu)), and the other to sheep (Bpp(ov)). While Bpp(hu) and the other classical bordetellae can efficiently colonize mice, Bpp(ov) strains are severely defective in their ability to colonize the murine respiratory tract. Bpp(ov) genomic analysis did not reveal the loss of adherence genes, but substantial mutations and deletions of multiple genes involved in the production of O-antigen, which is required to prevent complement deposition on B. bronchiseptica and Bpp(hu) strains. Bpp(ov) lacks O-antigen and, like O-antigen mutants of other bordetellae, is highly sensitive to murine complement-mediated killing in vitro. Based on these results, we hypothesized that Bpp(ov) failed to colonize mice because of its sensitivity to murine complement. Consistent with this, the Bpp(ov) defect in the colonization of wild type mice was not observed in mice lacking the central complement component C3. Furthermore, Bpp(ov) strains were highly susceptible to killing by murine complement, but not by sheep complement. These data demonstrate that the failure of Bpp(ov) to colonize mice is due to sensitivity to murine, but not sheep, complement, providing a mechanistic example of how specialization that accompanies expansion in one host can limit host range. |
format | Online Article Text |
id | pubmed-4497623 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44976232015-07-14 Host Specificity of Ovine Bordetella parapertussis and the Role of Complement Hester, Sara E. Goodfield, Laura L. Park, Jihye Feaga, Heather A. Ivanov, Yury V. Bendor, Liron Taylor, Dawn L. Harvill, Eric T. PLoS One Research Article The classical bordetellae are comprised of three subspecies that differ from broad to very limited host specificity. Although several lineages appear to have specialized to particular host species, most retain the ability to colonize and grow in mice, providing a powerful common experimental model to study their differences. One of the subspecies, Bordetella parapertussis, is composed of two distinct clades that have specialized to different hosts: one to humans (Bpp(hu)), and the other to sheep (Bpp(ov)). While Bpp(hu) and the other classical bordetellae can efficiently colonize mice, Bpp(ov) strains are severely defective in their ability to colonize the murine respiratory tract. Bpp(ov) genomic analysis did not reveal the loss of adherence genes, but substantial mutations and deletions of multiple genes involved in the production of O-antigen, which is required to prevent complement deposition on B. bronchiseptica and Bpp(hu) strains. Bpp(ov) lacks O-antigen and, like O-antigen mutants of other bordetellae, is highly sensitive to murine complement-mediated killing in vitro. Based on these results, we hypothesized that Bpp(ov) failed to colonize mice because of its sensitivity to murine complement. Consistent with this, the Bpp(ov) defect in the colonization of wild type mice was not observed in mice lacking the central complement component C3. Furthermore, Bpp(ov) strains were highly susceptible to killing by murine complement, but not by sheep complement. These data demonstrate that the failure of Bpp(ov) to colonize mice is due to sensitivity to murine, but not sheep, complement, providing a mechanistic example of how specialization that accompanies expansion in one host can limit host range. Public Library of Science 2015-07-09 /pmc/articles/PMC4497623/ /pubmed/26158540 http://dx.doi.org/10.1371/journal.pone.0130964 Text en © 2015 Hester et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Hester, Sara E. Goodfield, Laura L. Park, Jihye Feaga, Heather A. Ivanov, Yury V. Bendor, Liron Taylor, Dawn L. Harvill, Eric T. Host Specificity of Ovine Bordetella parapertussis and the Role of Complement |
title | Host Specificity of Ovine Bordetella parapertussis and the Role of Complement |
title_full | Host Specificity of Ovine Bordetella parapertussis and the Role of Complement |
title_fullStr | Host Specificity of Ovine Bordetella parapertussis and the Role of Complement |
title_full_unstemmed | Host Specificity of Ovine Bordetella parapertussis and the Role of Complement |
title_short | Host Specificity of Ovine Bordetella parapertussis and the Role of Complement |
title_sort | host specificity of ovine bordetella parapertussis and the role of complement |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497623/ https://www.ncbi.nlm.nih.gov/pubmed/26158540 http://dx.doi.org/10.1371/journal.pone.0130964 |
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