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Neorickettsia sennetsu as a Neglected Cause of Fever in South-East Asia

Neorickettsia sennetsu infection is rarely recognized, with less than 100 globally reported patients over the last 50 years. The disease is thought to be contracted by eating raw fish, a staple of many South-East Asian cuisines. In 2009, the first patient with sennetsu was identified in the Lao PDR...

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Autores principales: Dittrich, Sabine, Phuklia, Weerawat, Turner, Gareth D. H., Rattanavong, Sayaphet, Chansamouth, Vilada, Dumler, Stephen J., Ferguson, David J. P., Paris, Daniel H., Newton, Paul N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497638/
https://www.ncbi.nlm.nih.gov/pubmed/26158273
http://dx.doi.org/10.1371/journal.pntd.0003908
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author Dittrich, Sabine
Phuklia, Weerawat
Turner, Gareth D. H.
Rattanavong, Sayaphet
Chansamouth, Vilada
Dumler, Stephen J.
Ferguson, David J. P.
Paris, Daniel H.
Newton, Paul N.
author_facet Dittrich, Sabine
Phuklia, Weerawat
Turner, Gareth D. H.
Rattanavong, Sayaphet
Chansamouth, Vilada
Dumler, Stephen J.
Ferguson, David J. P.
Paris, Daniel H.
Newton, Paul N.
author_sort Dittrich, Sabine
collection PubMed
description Neorickettsia sennetsu infection is rarely recognized, with less than 100 globally reported patients over the last 50 years. The disease is thought to be contracted by eating raw fish, a staple of many South-East Asian cuisines. In 2009, the first patient with sennetsu was identified in the Lao PDR (Laos), raising the question as to how common this organism and related species are in patients presenting with fever. We investigated the frequency of N. sennetsu infection at hospitals in diverse areas of Laos. Consenting febrile hospital inpatients from central (Vientiane: n = 1,013), northern (Luang Namtha: n = 453) and southern (Salavan: n = 171) Laos were screened by PCR for N. sennetsu, if no previous positive direct diagnostic test was available. A PCR-restriction fragment length polymorphism assay was developed to differentiate between N. sennetsu, Ehrlichia chaffeensis and Anaplasma phagocytophilum. To allow more detailed studies of N. sennetsu, culture was successfully established using a reference strain (ATCC VR-367), identifying a canine-macrophage cell line (DH82) to be most suitable to visually identify infection. After screening, N. sennetsu was identified and sequence confirmed in four (4/1,637; 0.2%) Lao patients. Despite the previously identified high seroprevalence of N. sennetsu antibodies in the Lao population (~17%), acute N. sennetsu infection with sufficient clinical signs to prompt hospitalization appears to be rare. The reservoir, zoonotic cycle and pathogenicity of N. sennetsu remain unclear and require further investigations.
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spelling pubmed-44976382015-07-14 Neorickettsia sennetsu as a Neglected Cause of Fever in South-East Asia Dittrich, Sabine Phuklia, Weerawat Turner, Gareth D. H. Rattanavong, Sayaphet Chansamouth, Vilada Dumler, Stephen J. Ferguson, David J. P. Paris, Daniel H. Newton, Paul N. PLoS Negl Trop Dis Research Article Neorickettsia sennetsu infection is rarely recognized, with less than 100 globally reported patients over the last 50 years. The disease is thought to be contracted by eating raw fish, a staple of many South-East Asian cuisines. In 2009, the first patient with sennetsu was identified in the Lao PDR (Laos), raising the question as to how common this organism and related species are in patients presenting with fever. We investigated the frequency of N. sennetsu infection at hospitals in diverse areas of Laos. Consenting febrile hospital inpatients from central (Vientiane: n = 1,013), northern (Luang Namtha: n = 453) and southern (Salavan: n = 171) Laos were screened by PCR for N. sennetsu, if no previous positive direct diagnostic test was available. A PCR-restriction fragment length polymorphism assay was developed to differentiate between N. sennetsu, Ehrlichia chaffeensis and Anaplasma phagocytophilum. To allow more detailed studies of N. sennetsu, culture was successfully established using a reference strain (ATCC VR-367), identifying a canine-macrophage cell line (DH82) to be most suitable to visually identify infection. After screening, N. sennetsu was identified and sequence confirmed in four (4/1,637; 0.2%) Lao patients. Despite the previously identified high seroprevalence of N. sennetsu antibodies in the Lao population (~17%), acute N. sennetsu infection with sufficient clinical signs to prompt hospitalization appears to be rare. The reservoir, zoonotic cycle and pathogenicity of N. sennetsu remain unclear and require further investigations. Public Library of Science 2015-07-09 /pmc/articles/PMC4497638/ /pubmed/26158273 http://dx.doi.org/10.1371/journal.pntd.0003908 Text en © 2015 Dittrich et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Dittrich, Sabine
Phuklia, Weerawat
Turner, Gareth D. H.
Rattanavong, Sayaphet
Chansamouth, Vilada
Dumler, Stephen J.
Ferguson, David J. P.
Paris, Daniel H.
Newton, Paul N.
Neorickettsia sennetsu as a Neglected Cause of Fever in South-East Asia
title Neorickettsia sennetsu as a Neglected Cause of Fever in South-East Asia
title_full Neorickettsia sennetsu as a Neglected Cause of Fever in South-East Asia
title_fullStr Neorickettsia sennetsu as a Neglected Cause of Fever in South-East Asia
title_full_unstemmed Neorickettsia sennetsu as a Neglected Cause of Fever in South-East Asia
title_short Neorickettsia sennetsu as a Neglected Cause of Fever in South-East Asia
title_sort neorickettsia sennetsu as a neglected cause of fever in south-east asia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497638/
https://www.ncbi.nlm.nih.gov/pubmed/26158273
http://dx.doi.org/10.1371/journal.pntd.0003908
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