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The Diagnostic Accuracy of the M2 Pyruvate Kinase Quick Stool Test- A Rapid Office Based Assay Test for the Detection of Colorectal Cancer

BACKGROUND: M2 pyruvate kinase (M2PK) is an oncoprotein secreted by colorectal cancers in stools. This the first report on the accuracy of a rapid stool test in the detection of colorectal cancer (CRC). OBJECTIVE: To determine the sensitivity, specificity and positive and negative predictive value o...

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Autores principales: Sithambaram, Suresh, Hilmi, Ida, Goh, Khean-Lee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497640/
https://www.ncbi.nlm.nih.gov/pubmed/26158845
http://dx.doi.org/10.1371/journal.pone.0131616
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author Sithambaram, Suresh
Hilmi, Ida
Goh, Khean-Lee
author_facet Sithambaram, Suresh
Hilmi, Ida
Goh, Khean-Lee
author_sort Sithambaram, Suresh
collection PubMed
description BACKGROUND: M2 pyruvate kinase (M2PK) is an oncoprotein secreted by colorectal cancers in stools. This the first report on the accuracy of a rapid stool test in the detection of colorectal cancer (CRC). OBJECTIVE: To determine the sensitivity, specificity and positive and negative predictive value of a rapid, point of care stool test M2 PK- the M2PK Quick. METHODS: Consecutive cases of endoscopically diagnosed and histological proven CRC were recruited. Stools were collected by patients and tested with the immunochromatographic M2PK Quick Test (Schebo Biotech AC, Giessen, Germany). Controls were consecutively chosen from patients without any significant colorectal or gastrointestinal disease undergoing colonoscopy. CRC was staged according to the AJCC staging manual (7(th) Edition) and location of tumor defined as proximal or distal. RESULTS: The sensitivity, specificity, positive predictive value, negative predictive value and overall accuracy were: 93%, 97.5%, 94.9%, 96.5% and 96.0% respectively. The positive predictive value for proximal tumors was significantly lower compared to distal tumors. No differences were seen between the different stages of the tumor. CONCLUSIONS: The M2-PK Quick, rapid, point-of-care test is a highly accurate test in the detection of CRC. It is easy and convenient to perform and a useful diagnostic test for the detection of CRC in a clinical practice setting.
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spelling pubmed-44976402015-07-14 The Diagnostic Accuracy of the M2 Pyruvate Kinase Quick Stool Test- A Rapid Office Based Assay Test for the Detection of Colorectal Cancer Sithambaram, Suresh Hilmi, Ida Goh, Khean-Lee PLoS One Research Article BACKGROUND: M2 pyruvate kinase (M2PK) is an oncoprotein secreted by colorectal cancers in stools. This the first report on the accuracy of a rapid stool test in the detection of colorectal cancer (CRC). OBJECTIVE: To determine the sensitivity, specificity and positive and negative predictive value of a rapid, point of care stool test M2 PK- the M2PK Quick. METHODS: Consecutive cases of endoscopically diagnosed and histological proven CRC were recruited. Stools were collected by patients and tested with the immunochromatographic M2PK Quick Test (Schebo Biotech AC, Giessen, Germany). Controls were consecutively chosen from patients without any significant colorectal or gastrointestinal disease undergoing colonoscopy. CRC was staged according to the AJCC staging manual (7(th) Edition) and location of tumor defined as proximal or distal. RESULTS: The sensitivity, specificity, positive predictive value, negative predictive value and overall accuracy were: 93%, 97.5%, 94.9%, 96.5% and 96.0% respectively. The positive predictive value for proximal tumors was significantly lower compared to distal tumors. No differences were seen between the different stages of the tumor. CONCLUSIONS: The M2-PK Quick, rapid, point-of-care test is a highly accurate test in the detection of CRC. It is easy and convenient to perform and a useful diagnostic test for the detection of CRC in a clinical practice setting. Public Library of Science 2015-07-09 /pmc/articles/PMC4497640/ /pubmed/26158845 http://dx.doi.org/10.1371/journal.pone.0131616 Text en © 2015 Sithambaram et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sithambaram, Suresh
Hilmi, Ida
Goh, Khean-Lee
The Diagnostic Accuracy of the M2 Pyruvate Kinase Quick Stool Test- A Rapid Office Based Assay Test for the Detection of Colorectal Cancer
title The Diagnostic Accuracy of the M2 Pyruvate Kinase Quick Stool Test- A Rapid Office Based Assay Test for the Detection of Colorectal Cancer
title_full The Diagnostic Accuracy of the M2 Pyruvate Kinase Quick Stool Test- A Rapid Office Based Assay Test for the Detection of Colorectal Cancer
title_fullStr The Diagnostic Accuracy of the M2 Pyruvate Kinase Quick Stool Test- A Rapid Office Based Assay Test for the Detection of Colorectal Cancer
title_full_unstemmed The Diagnostic Accuracy of the M2 Pyruvate Kinase Quick Stool Test- A Rapid Office Based Assay Test for the Detection of Colorectal Cancer
title_short The Diagnostic Accuracy of the M2 Pyruvate Kinase Quick Stool Test- A Rapid Office Based Assay Test for the Detection of Colorectal Cancer
title_sort diagnostic accuracy of the m2 pyruvate kinase quick stool test- a rapid office based assay test for the detection of colorectal cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497640/
https://www.ncbi.nlm.nih.gov/pubmed/26158845
http://dx.doi.org/10.1371/journal.pone.0131616
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