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Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference

Interleukin (IL)-8 is a potent neutrophil chemotactic factor and a crucial mediator in neutrophil-dependent inflammation. Various cell types produce IL-8, either in response to external stimuli such as cytokines or bacterial infection, or after malignant transformation. Anti-IL-8 strategies have bee...

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Autores principales: CAO, Huibi, WANG, Anan, MARTIN, Bernard, KOEHLER, David R, ZEITLIN, Pamela L, TANAWELL, A Keith, HU, Jim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497818/
https://www.ncbi.nlm.nih.gov/pubmed/15740640
http://dx.doi.org/10.1038/sj.cr.7290275
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author CAO, Huibi
WANG, Anan
MARTIN, Bernard
KOEHLER, David R
ZEITLIN, Pamela L
TANAWELL, A Keith
HU, Jim
author_facet CAO, Huibi
WANG, Anan
MARTIN, Bernard
KOEHLER, David R
ZEITLIN, Pamela L
TANAWELL, A Keith
HU, Jim
author_sort CAO, Huibi
collection PubMed
description Interleukin (IL)-8 is a potent neutrophil chemotactic factor and a crucial mediator in neutrophil-dependent inflammation. Various cell types produce IL-8, either in response to external stimuli such as cytokines or bacterial infection, or after malignant transformation. Anti-IL-8 strategies have been considered for anti-inflammatory therapy. In this paper we demonstrate that the RNA interference technique can be used to efficiently down-regulate IL-8 protein expression in airway epithelial cells. We used a helper-dependent adenoviral vector to express a small hairpin (sh)RNA targeting human IL-8 in cultured airway epithelial cells (IB3-1, Cftr(−/−); C38, Cftr-corrected) stimulated with TNF-α, IL-1β or heat-inactivated Burkholderia cenocepacia. Stimulated IL-8 expression in IB3-1 and C38 cells was significantly reduced by shRNA expression. The shRNA targeting IL-8 had no effect on the activation of NF-κB, or on the protein levels of IκB or IL-6, suggesting that this anti-IL-8 strategy was highly specific, and therefore may offer potential for the treatment of inflammatory diseases. SUPPLEMENTARY INFORMATION: The online version of this article (doi:10.1038/sj.cr.7290275) contains supplementary material, which is available to authorized users.
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spelling pubmed-44978182015-07-09 Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference CAO, Huibi WANG, Anan MARTIN, Bernard KOEHLER, David R ZEITLIN, Pamela L TANAWELL, A Keith HU, Jim Cell Res Article Interleukin (IL)-8 is a potent neutrophil chemotactic factor and a crucial mediator in neutrophil-dependent inflammation. Various cell types produce IL-8, either in response to external stimuli such as cytokines or bacterial infection, or after malignant transformation. Anti-IL-8 strategies have been considered for anti-inflammatory therapy. In this paper we demonstrate that the RNA interference technique can be used to efficiently down-regulate IL-8 protein expression in airway epithelial cells. We used a helper-dependent adenoviral vector to express a small hairpin (sh)RNA targeting human IL-8 in cultured airway epithelial cells (IB3-1, Cftr(−/−); C38, Cftr-corrected) stimulated with TNF-α, IL-1β or heat-inactivated Burkholderia cenocepacia. Stimulated IL-8 expression in IB3-1 and C38 cells was significantly reduced by shRNA expression. The shRNA targeting IL-8 had no effect on the activation of NF-κB, or on the protein levels of IκB or IL-6, suggesting that this anti-IL-8 strategy was highly specific, and therefore may offer potential for the treatment of inflammatory diseases. SUPPLEMENTARY INFORMATION: The online version of this article (doi:10.1038/sj.cr.7290275) contains supplementary material, which is available to authorized users. Nature Publishing Group UK 2005-02 /pmc/articles/PMC4497818/ /pubmed/15740640 http://dx.doi.org/10.1038/sj.cr.7290275 Text en © Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences 2005 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Article
CAO, Huibi
WANG, Anan
MARTIN, Bernard
KOEHLER, David R
ZEITLIN, Pamela L
TANAWELL, A Keith
HU, Jim
Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference
title Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference
title_full Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference
title_fullStr Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference
title_full_unstemmed Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference
title_short Down-regulation of IL-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated RNA interference
title_sort down-regulation of il-8 expression in human airway epithelial cells through helper-dependent adenoviral-mediated rna interference
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4497818/
https://www.ncbi.nlm.nih.gov/pubmed/15740640
http://dx.doi.org/10.1038/sj.cr.7290275
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