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Lymphangiogenesis in cervical cancer evaluated by expression of the VEGF-C gene in clinical stage IB-IIIB

INTRODUCTION: The aim of the present study was to evaluate the profile of VEGF-C gene expression in particular stages of cervical cancer (IB-IIIB) and to estimate the correlation between VEGF-C mRNA quantity profile and clinical stage. MATERIAL AND METHODS: Material for molecular analysis consisted...

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Autores principales: Franc, Magdalena, Kachel-Flis, Agata, Michalski, Bogdan, Fila-Daniłow, Anna, Mazurek, Urszula, Michalski, Mateusz, Michalska, Anna, Kuczerawy, Ilona, Skrzypulec-Plinta, Violetta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Termedia Publishing House 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498023/
https://www.ncbi.nlm.nih.gov/pubmed/26327898
http://dx.doi.org/10.5114/pm.2015.49397
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author Franc, Magdalena
Kachel-Flis, Agata
Michalski, Bogdan
Fila-Daniłow, Anna
Mazurek, Urszula
Michalski, Mateusz
Michalska, Anna
Kuczerawy, Ilona
Skrzypulec-Plinta, Violetta
author_facet Franc, Magdalena
Kachel-Flis, Agata
Michalski, Bogdan
Fila-Daniłow, Anna
Mazurek, Urszula
Michalski, Mateusz
Michalska, Anna
Kuczerawy, Ilona
Skrzypulec-Plinta, Violetta
author_sort Franc, Magdalena
collection PubMed
description INTRODUCTION: The aim of the present study was to evaluate the profile of VEGF-C gene expression in particular stages of cervical cancer (IB-IIIB) and to estimate the correlation between VEGF-C mRNA quantity profile and clinical stage. MATERIAL AND METHODS: Material for molecular analysis consisted of cervical cancer tissue specimens collected from 38 women (10, 15, 13 cases were classified as IB, IIB and IIIB, respectively). The control group was composed of normal cervical tissues collected from 10 women who underwent hysterectomy for non-oncological reasons. The number of VEGF-C mRNA copies in particular groups was estimated by the reverse transcription quantitative polymerase chain reaction (RT-qPCR) method. RESULTS: In the control group the average number of mRNA copies was 134 ± 36 (median: 106), in a group with stage IB it was 16 077 ± 7090 (median: 580), for stage IIB – 35 019 ± 8945 (median: 40 870). The highest number of mRNA VEGF-C copies was derived in a group of patients with cervical cancer of stage IIIB. The average quantity was 56 155 ± 12 470, whereas median 55 981. A statistically significantly higher level of VEGF-C gene expression was disclosed in cervical cancer specimens with stage IIB and IIIB than in the control group. In stage IIIB, the VEGF-C gene expression was significantly higher than in specimens derived from individuals in stage IB. CONCLUSIONS: In squamous cell carcinoma of the uterine cervix of stage IB-IIIB genes involved in lymphangiogenesis, especially VEGF-C, are expressed, which expression increases as the clinical stage of cervical cancer is higher.
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spelling pubmed-44980232015-08-31 Lymphangiogenesis in cervical cancer evaluated by expression of the VEGF-C gene in clinical stage IB-IIIB Franc, Magdalena Kachel-Flis, Agata Michalski, Bogdan Fila-Daniłow, Anna Mazurek, Urszula Michalski, Mateusz Michalska, Anna Kuczerawy, Ilona Skrzypulec-Plinta, Violetta Prz Menopauzalny Original Paper INTRODUCTION: The aim of the present study was to evaluate the profile of VEGF-C gene expression in particular stages of cervical cancer (IB-IIIB) and to estimate the correlation between VEGF-C mRNA quantity profile and clinical stage. MATERIAL AND METHODS: Material for molecular analysis consisted of cervical cancer tissue specimens collected from 38 women (10, 15, 13 cases were classified as IB, IIB and IIIB, respectively). The control group was composed of normal cervical tissues collected from 10 women who underwent hysterectomy for non-oncological reasons. The number of VEGF-C mRNA copies in particular groups was estimated by the reverse transcription quantitative polymerase chain reaction (RT-qPCR) method. RESULTS: In the control group the average number of mRNA copies was 134 ± 36 (median: 106), in a group with stage IB it was 16 077 ± 7090 (median: 580), for stage IIB – 35 019 ± 8945 (median: 40 870). The highest number of mRNA VEGF-C copies was derived in a group of patients with cervical cancer of stage IIIB. The average quantity was 56 155 ± 12 470, whereas median 55 981. A statistically significantly higher level of VEGF-C gene expression was disclosed in cervical cancer specimens with stage IIB and IIIB than in the control group. In stage IIIB, the VEGF-C gene expression was significantly higher than in specimens derived from individuals in stage IB. CONCLUSIONS: In squamous cell carcinoma of the uterine cervix of stage IB-IIIB genes involved in lymphangiogenesis, especially VEGF-C, are expressed, which expression increases as the clinical stage of cervical cancer is higher. Termedia Publishing House 2015-06-22 2015-06 /pmc/articles/PMC4498023/ /pubmed/26327898 http://dx.doi.org/10.5114/pm.2015.49397 Text en Copyright © 2015 Termedia http://creativecommons.org/licenses/by-nc-nd/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 3.0 Unported License, permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Paper
Franc, Magdalena
Kachel-Flis, Agata
Michalski, Bogdan
Fila-Daniłow, Anna
Mazurek, Urszula
Michalski, Mateusz
Michalska, Anna
Kuczerawy, Ilona
Skrzypulec-Plinta, Violetta
Lymphangiogenesis in cervical cancer evaluated by expression of the VEGF-C gene in clinical stage IB-IIIB
title Lymphangiogenesis in cervical cancer evaluated by expression of the VEGF-C gene in clinical stage IB-IIIB
title_full Lymphangiogenesis in cervical cancer evaluated by expression of the VEGF-C gene in clinical stage IB-IIIB
title_fullStr Lymphangiogenesis in cervical cancer evaluated by expression of the VEGF-C gene in clinical stage IB-IIIB
title_full_unstemmed Lymphangiogenesis in cervical cancer evaluated by expression of the VEGF-C gene in clinical stage IB-IIIB
title_short Lymphangiogenesis in cervical cancer evaluated by expression of the VEGF-C gene in clinical stage IB-IIIB
title_sort lymphangiogenesis in cervical cancer evaluated by expression of the vegf-c gene in clinical stage ib-iiib
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498023/
https://www.ncbi.nlm.nih.gov/pubmed/26327898
http://dx.doi.org/10.5114/pm.2015.49397
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