Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI

This short review aims to summarize the current developments and applications of mass spectrometry-based methods for in situ profiling and imaging of plants with minimal or no sample pre-treatment or manipulation. Infrared-laser ablation electrospray ionization and UV-laser desorption/ionization met...

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Detalles Bibliográficos
Autores principales: Bartels, Benjamin, Svatoš, Aleš
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2015
Materias:
Plant Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498035/
https://www.ncbi.nlm.nih.gov/pubmed/26217345
http://dx.doi.org/10.3389/fpls.2015.00471
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author Bartels, Benjamin
Svatoš, Aleš
author_facet Bartels, Benjamin
Svatoš, Aleš
author_sort Bartels, Benjamin
collection PubMed
description This short review aims to summarize the current developments and applications of mass spectrometry-based methods for in situ profiling and imaging of plants with minimal or no sample pre-treatment or manipulation. Infrared-laser ablation electrospray ionization and UV-laser desorption/ionization methods are reviewed. The underlying mechanisms of the ionization techniques–namely, laser ablation of biological samples and electrospray ionization–as well as variations of the LAESI ion source for specific targets of interest are described.
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spelling pubmed-44980352015-07-27 Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI Bartels, Benjamin Svatoš, Aleš Front Plant Sci Plant Science This short review aims to summarize the current developments and applications of mass spectrometry-based methods for in situ profiling and imaging of plants with minimal or no sample pre-treatment or manipulation. Infrared-laser ablation electrospray ionization and UV-laser desorption/ionization methods are reviewed. The underlying mechanisms of the ionization techniques–namely, laser ablation of biological samples and electrospray ionization–as well as variations of the LAESI ion source for specific targets of interest are described. Frontiers Media S.A. 2015-07-10 /pmc/articles/PMC4498035/ /pubmed/26217345 http://dx.doi.org/10.3389/fpls.2015.00471 Text en Copyright © 2015 Bartels and Svatoš. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bartels, Benjamin
Svatoš, Aleš
Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI
title Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI
title_full Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI
title_fullStr Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI
title_full_unstemmed Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI
title_short Spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (MSI) techniques: LDI-MSI and LAESI
title_sort spatially resolved in vivo plant metabolomics by laser ablation-based mass spectrometry imaging (msi) techniques: ldi-msi and laesi
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4498035/
https://www.ncbi.nlm.nih.gov/pubmed/26217345
http://dx.doi.org/10.3389/fpls.2015.00471
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AT svatosales spatiallyresolvedinvivoplantmetabolomicsbylaserablationbasedmassspectrometryimagingmsitechniquesldimsiandlaesi

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